GenomeNet

Database: RefSeq
Entry: NP_037499
LinkDB: NP_037499
Original site: NP_037499 
LOCUS       NP_037499                808 aa            linear   PRI 27-APR-2025
DEFINITION  anaphase-promoting complex subunit 4 isoform 2 [Homo sapiens].
ACCESSION   NP_037499
VERSION     NP_037499.2
DBSOURCE    REFSEQ: accession NM_013367.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 808)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (residues 1 to 808)
  AUTHORS   Wu,H., Zhang,X., Shen,Q., Liu,Y., Gao,Y., Wang,G., Lv,M., Hua,R.,
            Xu,Y., Zhou,P., Wei,Z., Tao,F., He,X., Cao,Y. and Liu,M.
  TITLE     A homozygous loss-of-function mutation in FBXO43 causes human
            non-obstructive azoospermia
  JOURNAL   Clin Genet 101 (1), 55-64 (2022)
   PUBMED   34595750
REFERENCE   3  (residues 1 to 808)
  AUTHORS   Lee,C.C., Li,B., Yu,H. and Matunis,M.J.
  TITLE     Sumoylation promotes optimal APC/C Activation and Timely Anaphase
  JOURNAL   Elife 7, e29539 (2018)
   PUBMED   29517484
  REMARK    GeneRIF: We have also identified a functionally important SUMO
            interacting motif in the cullin-homology domain of APC2 located
            near the APC4 sumoylation sites and APC/C catalytic core. Our
            findings provide evidence of an important regulatory role for SUMO
            modification and binding in affecting APC/C activation and mitotic
            exit.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 808)
  AUTHORS   Yau,R.G., Doerner,K., Castellanos,E.R., Haakonsen,D.L., Werner,A.,
            Wang,N., Yang,X.W., Martinez-Martin,N., Matsumoto,M.L., Dixit,V.M.
            and Rape,M.
  TITLE     Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle
            and Protein Quality Control
  JOURNAL   Cell 171 (4), 918-933 (2017)
   PUBMED   29033132
REFERENCE   5  (residues 1 to 808)
  AUTHORS   Zhang,S., Chang,L., Alfieri,C., Zhang,Z., Yang,J., Maslen,S.,
            Skehel,M. and Barford,D.
  TITLE     Molecular mechanism of APC/C activation by mitotic phosphorylation
  JOURNAL   Nature 533 (7602), 260-264 (2016)
   PUBMED   27120157
REFERENCE   6  (residues 1 to 808)
  AUTHORS   Lukas,C., Sorensen,C.S., Kramer,E., Santoni-Rugiu,E., Lindeneg,C.,
            Peters,J.M., Bartek,J. and Lukas,J.
  TITLE     Accumulation of cyclin B1 requires E2F and cyclin-A-dependent
            rearrangement of the anaphase-promoting complex
  JOURNAL   Nature 401 (6755), 815-818 (1999)
   PUBMED   10548110
REFERENCE   7  (residues 1 to 808)
  AUTHORS   Gieffers,C., Peters,B.H., Kramer,E.R., Dotti,C.G. and Peters,J.M.
  TITLE     Expression of the CDH1-associated form of the anaphase-promoting
            complex in postmitotic neurons
  JOURNAL   Proc Natl Acad Sci U S A 96 (20), 11317-11322 (1999)
   PUBMED   10500174
REFERENCE   8  (residues 1 to 808)
  AUTHORS   Grossberger,R., Gieffers,C., Zachariae,W., Podtelejnikov,A.V.,
            Schleiffer,A., Nasmyth,K., Mann,M. and Peters,J.M.
  TITLE     Characterization of the DOC1/APC10 subunit of the yeast and the
            human anaphase-promoting complex
  JOURNAL   J Biol Chem 274 (20), 14500-14507 (1999)
   PUBMED   10318877
REFERENCE   9  (residues 1 to 808)
  AUTHORS   Yu,H., Peters,J.M., King,R.W., Page,A.M., Hieter,P. and
            Kirschner,M.W.
  TITLE     Identification of a cullin homology region in a subunit of the
            anaphase-promoting complex
  JOURNAL   Science 279 (5354), 1219-1222 (1998)
   PUBMED   9469815
REFERENCE   10 (residues 1 to 808)
  AUTHORS   Tekolf,W.A., Biddison,W.E., Aster,R.D. and Shaw,S.
  TITLE     Two subgroups of HLA Bw44 defined by cell-mediated lympholysis that
            differ in Bw44 expression on platelets and in patterns of genetic
            linkage disequilibrium
  JOURNAL   J Immunol 129 (4), 1474-1478 (1982)
   PUBMED   6180011
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI546948.1, BC059383.1 and
            CA312572.1.
            
            On Jan 26, 2004 this sequence version replaced NP_037499.1.
            
            Summary: A large protein complex, termed the anaphase-promoting
            complex (APC), or the cyclosome, promotes metaphase-anaphase
            transition by ubiquitinating its specific substrates such as
            mitotic cyclins and anaphase inhibitor, which are subsequently
            degraded by the 26S proteasome. Biochemical studies have shown that
            the vertebrate APC contains eight subunits. The composition of the
            APC is highly conserved in organisms from yeast to humans. The
            exact function of this gene product is not known. Two transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Nov 2013].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice junction at the 3' end of an exon compared to variant 1. The
            resulting isoform (2) has the same N- and C-termini but is 1 aa
            shorter compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC059383.1, SRR1803612.59956.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000315368.8/ ENSP00000318775.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p15.2"
     Protein         1..808
                     /product="anaphase-promoting complex subunit 4 isoform 2"
                     /note="cyclosome subunit 4"
                     /calculated_mol_wt=91986
     Region          24..59
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          26..118
                     /region_name="ANAPC4_WD40"
                     /note="Anaphase-promoting complex subunit 4 WD40 domain;
                     pfam12894"
                     /db_xref="CDD:403945"
     Region          68..102
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          232..431
                     /region_name="ANAPC4"
                     /note="Anaphase-promoting complex, cyclosome, subunit 4;
                     pfam12896"
                     /db_xref="CDD:463744"
     Site            469
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000269|PubMed:14657031; propagated from
                     UniProtKB/Swiss-Prot (Q9UJX5.2)"
     Site            757
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:19690332; propagated from
                     UniProtKB/Swiss-Prot (Q9UJX5.2)"
     Site            758
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:19690332; propagated from
                     UniProtKB/Swiss-Prot (Q9UJX5.2)"
     Site            777
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:19690332,
                     ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q9UJX5.2)"
     Site            779
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000269|PubMed:14657031; propagated from
                     UniProtKB/Swiss-Prot (Q9UJX5.2)"
     CDS             1..808
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /coded_by="NM_013367.3:93..2519"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /db_xref="CCDS:CCDS3434.1"
                     /db_xref="GeneID:29945"
                     /db_xref="HGNC:HGNC:19990"
                     /db_xref="MIM:606947"
ORIGIN      
        1 mlrfptcfps frvvgekqlp qeiiflvwsp krdlialant agevllhrla sfhrvwsfpp
       61 nentgkevtc lawrpdgkll afaladtkki vlcdvekpes lhsfsveapv scmhwmevtv
      121 essvltsfyn aedesnlllp klptlpknys ntskifseen sdeiikllgd vrlnilvlgg
      181 ssgfielyay gmfkiarvtg iagtclalcl ssdlkslsvv tevstngase vsyfqletnl
      241 lysflpevtr markfthisa llqyinlslt cmceaweeil mqmdsrltkf vqekntttsv
      301 qdefmhlllw gkasaelqtl lmnqltvkgl kklgqsiess yssiqklvis hlqsgsesll
      361 yhlselkgma swkqkyeplg ldaagieeai tavgsfilka nellqvidss mknfkaffrw
      421 lyvamlrmte dhvlpelnkm tqkditfvae fltehfneap dlynrkgkyf nvervgqylk
      481 dedddlvspp ntegnqwydf lqnsshlkes pllfpyyprk slhfvkrrme niidqclqkp
      541 advigksmnq aiciplyrdt rsedstrrlf kfpflwnnkt snlhyllfti ledslykmci
      601 lrrhtdisqs vsngliaikf gsftyattek vrrsiyscld aqfyddetvt vvlkdtvgre
      661 grdrllvqlp lslvynseds aeyqftgtys trldeqcsai ptrtmhfekh wrllesmkaq
      721 yvagngfrkv scvlssnlrh vrvfemdidd eweldessde eeeasnkpvk ikeevlsese
      781 aenqqagaaa lapeivikve kldpelds
//
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