Database: RefSeq
Entry: NP_037499
LinkDB: NP_037499
Original site: NP_037499 
LOCUS       NP_037499                808 aa            linear   PRI 31-AUG-2020
DEFINITION  anaphase-promoting complex subunit 4 isoform 2 [Homo sapiens].
VERSION     NP_037499.2
DBSOURCE    REFSEQ: accession NM_013367.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 808)
  AUTHORS   Lee CC, Li B, Yu H and Matunis MJ.
  TITLE     Sumoylation promotes optimal APC/C Activation and Timely Anaphase
  JOURNAL   Elife 7, e29539 (2018)
   PUBMED   29517484
  REMARK    GeneRIF: We have also identified a functionally important SUMO
            interacting motif in the cullin-homology domain of APC2 located
            near the APC4 sumoylation sites and APC/C catalytic core. Our
            findings provide evidence of an important regulatory role for SUMO
            modification and binding in affecting APC/C activation and mitotic
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 808)
  AUTHORS   Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA,
            Weisswange I, Mansfeld J, Buchholz F, Hyman AA and Mann M.
  TITLE     A human interactome in three quantitative dimensions organized by
            stoichiometries and abundances
  JOURNAL   Cell 163 (3), 712-723 (2015)
   PUBMED   26496610
REFERENCE   3  (residues 1 to 808)
  AUTHORS   Chang L, Zhang Z, Yang J, McLaughlin SH and Barford D.
  TITLE     Atomic structure of the APC/C and its mechanism of protein
  JOURNAL   Nature 522 (7557), 450-454 (2015)
   PUBMED   26083744
REFERENCE   4  (residues 1 to 808)
  AUTHORS   Izawa D and Pines J.
  TITLE     The mitotic checkpoint complex binds a second CDC20 to inhibit
            active APC/C
  JOURNAL   Nature 517 (7536), 631-634 (2015)
   PUBMED   25383541
REFERENCE   5  (residues 1 to 808)
  AUTHORS   Sedgwick GG, Hayward DG, Di Fiore B, Pardo M, Yu L, Pines J and
            Nilsson J.
  TITLE     Mechanisms controlling the temporal degradation of Nek2A and Kif18A
            by the APC/C-Cdc20 complex
  JOURNAL   EMBO J. 32 (2), 303-314 (2013)
   PUBMED   23288039
REFERENCE   6  (residues 1 to 808)
  AUTHORS   Lukas C, Sorensen CS, Kramer E, Santoni-Rugiu E, Lindeneg C, Peters
            JM, Bartek J and Lukas J.
  TITLE     Accumulation of cyclin B1 requires E2F and cyclin-A-dependent
            rearrangement of the anaphase-promoting complex
  JOURNAL   Nature 401 (6755), 815-818 (1999)
   PUBMED   10548110
REFERENCE   7  (residues 1 to 808)
  AUTHORS   Gieffers C, Peters BH, Kramer ER, Dotti CG and Peters JM.
  TITLE     Expression of the CDH1-associated form of the anaphase-promoting
            complex in postmitotic neurons
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 96 (20), 11317-11322 (1999)
   PUBMED   10500174
REFERENCE   8  (residues 1 to 808)
  AUTHORS   Grossberger R, Gieffers C, Zachariae W, Podtelejnikov AV,
            Schleiffer A, Nasmyth K, Mann M and Peters JM.
  TITLE     Characterization of the DOC1/APC10 subunit of the yeast and the
            human anaphase-promoting complex
  JOURNAL   J. Biol. Chem. 274 (20), 14500-14507 (1999)
   PUBMED   10318877
REFERENCE   9  (residues 1 to 808)
  AUTHORS   Yu H, Peters JM, King RW, Page AM, Hieter P and Kirschner MW.
  TITLE     Identification of a cullin homology region in a subunit of the
            anaphase-promoting complex
  JOURNAL   Science 279 (5354), 1219-1222 (1998)
   PUBMED   9469815
REFERENCE   10 (residues 1 to 808)
  AUTHORS   Tekolf,W.A., Biddison,W.E., Aster,R.D. and Shaw,S.
  TITLE     Two subgroups of HLA Bw44 defined by cell-mediated lympholysis that
            differ in Bw44 expression on platelets and in patterns of genetic
            linkage disequilibrium
  JOURNAL   J. Immunol. 129 (4), 1474-1478 (1982)
   PUBMED   6180011
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI546948.1, BC059383.1 and
            On Jan 26, 2004 this sequence version replaced NP_037499.1.
            Summary: A large protein complex, termed the anaphase-promoting
            complex (APC), or the cyclosome, promotes metaphase-anaphase
            transition by ubiquitinating its specific substrates such as
            mitotic cyclins and anaphase inhibitor, which are subsequently
            degraded by the 26S proteasome. Biochemical studies have shown that
            the vertebrate APC contains eight subunits. The composition of the
            APC is highly conserved in organisms from yeast to humans. The
            exact function of this gene product is not known. Two transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Nov 2013].
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice junction at the 3' end of an exon compared to variant 1. The
            resulting isoform (2) has the same N- and C-termini but is 1 aa
            shorter compared to isoform 1.
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            Transcript exon combination :: BC059383.1, SRR1803612.59956.1
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
            MANE Ensembl match     :: ENST00000315368.8/ ENSP00000318775.3
            RefSeq Select criteria :: based on conservation, expression
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Homo sapiens"
     Protein         1..808
                     /product="anaphase-promoting complex subunit 4 isoform 2"
                     /note="cyclosome subunit 4"
     Region          10..57
                     /note="Anaphase-promoting complex subunit 4 WD40 domain;
     Region          24..59
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
     Region          <26..115
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl02567"
     Region          68..102
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
     Region          232..431
                     /note="Anaphase-promoting complex, cyclosome, subunit 4;
     Site            469
                     /evidence=ECO:0000269|PubMed:14657031; propagated from
                     UniProtKB/Swiss-Prot (Q9UJX5.2)"
     Site            757
                     /evidence=ECO:0000244|PubMed:19690332; propagated from
                     UniProtKB/Swiss-Prot (Q9UJX5.2)"
     Site            758
                     /evidence=ECO:0000244|PubMed:19690332; propagated from
                     UniProtKB/Swiss-Prot (Q9UJX5.2)"
     Site            777
                     ECO:0000244|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q9UJX5.2)"
     Site            779
                     /evidence=ECO:0000269|PubMed:14657031; propagated from
                     UniProtKB/Swiss-Prot (Q9UJX5.2)"
     CDS             1..808
                     /note="isoform 2 is encoded by transcript variant 2"
        1 mlrfptcfps frvvgekqlp qeiiflvwsp krdlialant agevllhrla sfhrvwsfpp
       61 nentgkevtc lawrpdgkll afaladtkki vlcdvekpes lhsfsveapv scmhwmevtv
      121 essvltsfyn aedesnlllp klptlpknys ntskifseen sdeiikllgd vrlnilvlgg
      181 ssgfielyay gmfkiarvtg iagtclalcl ssdlkslsvv tevstngase vsyfqletnl
      241 lysflpevtr markfthisa llqyinlslt cmceaweeil mqmdsrltkf vqekntttsv
      301 qdefmhlllw gkasaelqtl lmnqltvkgl kklgqsiess yssiqklvis hlqsgsesll
      361 yhlselkgma swkqkyeplg ldaagieeai tavgsfilka nellqvidss mknfkaffrw
      421 lyvamlrmte dhvlpelnkm tqkditfvae fltehfneap dlynrkgkyf nvervgqylk
      481 dedddlvspp ntegnqwydf lqnsshlkes pllfpyyprk slhfvkrrme niidqclqkp
      541 advigksmnq aiciplyrdt rsedstrrlf kfpflwnnkt snlhyllfti ledslykmci
      601 lrrhtdisqs vsngliaikf gsftyattek vrrsiyscld aqfyddetvt vvlkdtvgre
      661 grdrllvqlp lslvynseds aeyqftgtys trldeqcsai ptrtmhfekh wrllesmkaq
      721 yvagngfrkv scvlssnlrh vrvfemdidd eweldessde eeeasnkpvk ikeevlsese
      781 aenqqagaaa lapeivikve kldpelds
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