GenomeNet

Database: RefSeq
Entry: NP_250828
LinkDB: NP_250828
Original site: NP_250828 
LOCUS       NP_250828                840 aa            linear   CON 13-FEB-2018
DEFINITION  multifunctional non-homologous end joining protein LigD
            [Pseudomonas aeruginosa PAO1].
ACCESSION   NP_250828
VERSION     NP_250828.1
DBLINK      BioProject: PRJNA57945
            Assembly: GCF_000006765.1
DBSOURCE    REFSEQ: accession NC_002516.2
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa PAO1
  ORGANISM  Pseudomonas aeruginosa PAO1
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (residues 1 to 840)
  AUTHORS   Winsor,G.L., Van Rossum,T., Lo,R., Khaira,B., Whiteside,M.D.,
            Hancock,R.E. and Brinkman,F.S.
  TITLE     Pseudomonas Genome Database: facilitating user-friendly,
            comprehensive comparisons of microbial genomes
  JOURNAL   Nucleic acids Res. 37 (DATABASE ISSUE), D483-D488 (2009)
   PUBMED   18978025
REFERENCE   2  (residues 1 to 840)
  AUTHORS   Cirz,R.T., O'Neill,B.M., Hammond,J.A., Head,S.R. and Romesberg,F.E.
  TITLE     Defining the Pseudomonas aeruginosa SOS response and its role in
            the global response to the antibiotic ciprofloxacin
  JOURNAL   J. Bacteriol. 188 (20), 7101-7110 (2006)
   PUBMED   17015649
REFERENCE   3  (residues 1 to 840)
  AUTHORS   Palmer,K.L., Mashburn,L.M., Singh,P.K. and Whiteley,M.
  TITLE     Cystic fibrosis sputum supports growth and cues key aspects of
            Pseudomonas aeruginosa physiology
  JOURNAL   J. Bacteriol. 187 (15), 5267-5277 (2005)
   PUBMED   16030221
REFERENCE   4  (residues 1 to 840)
  AUTHORS   Salunkhe,P., Smart,C.H., Morgan,J.A., Panagea,S., Walshaw,M.J.,
            Hart,C.A., Geffers,R., Tummler,B. and Winstanley,C.
  TITLE     A cystic fibrosis epidemic strain of Pseudomonas aeruginosa
            displays enhanced virulence and antimicrobial resistance
  JOURNAL   J. Bacteriol. 187 (14), 4908-4920 (2005)
   PUBMED   15995206
REFERENCE   5  (residues 1 to 840)
  AUTHORS   Filiatrault,M.J., Wagner,V.E., Bushnell,D., Haidaris,C.G.,
            Iglewski,B.H. and Passador,L.
  TITLE     Effect of anaerobiosis and nitrate on gene expression in
            Pseudomonas aeruginosa
  JOURNAL   Infect. Immun. 73 (6), 3764-3772 (2005)
   PUBMED   15908409
REFERENCE   6  (residues 1 to 840)
  AUTHORS   Rasmussen,T.B., Bjarnsholt,T., Skindersoe,M.E., Hentzer,M.,
            Kristoffersen,P., Kote,M., Nielsen,J., Eberl,L. and Givskov,M.
  TITLE     Screening for quorum-sensing inhibitors (QSI) by use of a novel
            genetic system, the QSI selector
  JOURNAL   J. Bacteriol. 187 (5), 1799-1814 (2005)
   PUBMED   15716452
REFERENCE   7  (residues 1 to 840)
  AUTHORS   Winsor,G.L., Lo,R., Sui,S.J., Ung,K.S., Huang,S., Cheng,D.,
            Ching,W.K., Hancock,R.E. and Brinkman,F.S.
  TITLE     Pseudomonas aeruginosa Genome Database and PseudoCap: facilitating
            community-based, continually updated, genome annotation
  JOURNAL   Nucleic acids Res. 33 (DATABASE ISSUE), D338-D343 (2005)
   PUBMED   15608211
REFERENCE   8  (residues 1 to 840)
  AUTHORS   Wagner,V.E., Bushnell,D., Passador,L., Brooks,A.I. and
            Iglewski,B.H.
  TITLE     Microarray analysis of Pseudomonas aeruginosa quorum-sensing
            regulons: effects of growth phase and environment
  JOURNAL   J. Bacteriol. 185 (7), 2080-2095 (2003)
   PUBMED   12644477
REFERENCE   9  (residues 1 to 840)
  AUTHORS   Schuster,M., Lostroh,C.P., Ogi,T. and Greenberg,E.P.
  TITLE     Identification, timing, and signal specificity of Pseudomonas
            aeruginosa quorum-controlled genes: a transcriptome analysis
  JOURNAL   J. Bacteriol. 185 (7), 2066-2079 (2003)
   PUBMED   12644476
REFERENCE   10 (residues 1 to 840)
  AUTHORS   Stover,C.K., Pham,X.Q., Erwin,A.L., Mizoguchi,S.D., Warrener,P.,
            Hickey,M.J., Brinkman,F.S., Hufnagle,W.O., Kowalik,D.J., Lagrou,M.,
            Garber,R.L., Goltry,L., Tolentino,E., Westbrock-Wadman,S., Yuan,Y.,
            Brody,L.L., Coulter,S.N., Folger,K.R., Kas,A., Larbig,K., Lim,R.,
            Smith,K., Spencer,D., Wong,G.K., Wu,Z., Paulsen,I.T., Reizer,J.,
            Saier,M.H., Hancock,R.E., Lory,S. and Olson,M.V.
  TITLE     Complete genome sequence of Pseudomonas aeruginosa PA01, an
            opportunistic pathogen
  JOURNAL   Nature 406 (6799), 959-964 (2000)
   PUBMED   10984043
REFERENCE   11 (residues 1 to 840)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2010) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   12 (residues 1 to 840)
  AUTHORS   Winsor,G.L., Hancock,R.E. and Brinkman,F.S.
  CONSRTM   Pseudomonas aeruginosa Community Annotation Project (PseudoCap)
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2010) Department of Molecular Biology and
            Biochemisry, Simon Fraser University, 8888 University Dr., Burnaby,
            British Columbia V5A 1S6, Canada
  REMARK    protein update by submitter
REFERENCE   13 (residues 1 to 840)
  AUTHORS   Winsor,G.L., Hancock,R.E. and Brinkman,F.S.
  CONSRTM   Pseudomonas aeruginosa Community Annotation Project (PseudoCap)
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2008) Department of Molecular Biology and
            Biochemisry, Simon Fraser University, 8888 University Dr., Burnaby,
            British Columbia V5A 1S6, Canada
  REMARK    protein update by submitter
REFERENCE   14 (residues 1 to 840)
  AUTHORS   Winsor,G.L., Hancock,R.E. and Brinkman,F.S.
  CONSRTM   Pseudomonas aeruginosa Community Annotation Project (PseudoCap)
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2006) Department of Molecular Biology and
            Biochemisry, Simon Fraser University, 8888 University Dr., Burnaby,
            British Columbia V5A 1S6, Canada
  REMARK    Sequence update by submitter
REFERENCE   15 (residues 1 to 840)
  AUTHORS   Ung,K.S., Hancock,R.E. and Brinkman,F.S.
  CONSRTM   Pseudomonas aeruginosa Community Annotation Project (PseudoCap)
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2003) Department of Molecular Biology and
            Biochemistry, Simon Fraser University, 8888 University Dr.,
            Burnaby, British Columbia V5A 1S6, Canada
REFERENCE   16 (residues 1 to 840)
  AUTHORS   Stover,C.K., Pham,X.-Q.T., Erwin,A.L., Mizoguchi,S.D., Warrener,P.,
            Hickey,M.J., Brinkman,F.S.L., Hufnagle,W.O., Kowalik,D.J.,
            Lagrou,M., Garber,R.L., Goltry,L., Tolentino,E.,
            Westbrock-Wadman,S., Yuan,Y., Brody,L.L., Coulter,S.N.,
            Folger,K.R., Kas,A., Larbig,K., Lim,R.M., Smith,K.A., Spencer,D.H.,
            Wong,G.K.-S., Wu,Z., Paulsen,I.T., Reizer,J., Saier,M.H.,
            Hancock,R.E.W., Lory,S. and Olson,M.V.
  CONSRTM   Pseudomonas aeruginosa Community Annotation Project (PseudoCap)
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2000) Department of Medicine and Genetics,
            University of Washington Genome Center, University Of Washington,
            Box 352145, Seattle, WA 98195, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to AAG05526.
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Pseudomonas aeruginosa PAO1"
                     /strain="PAO1"
                     /db_xref="taxon:208964"
     Protein         1..840
                     /product="multifunctional non-homologous end joining
                     protein LigD"
                     /EC_number="6.5.1.1"
                     /calculated_mol_wt=93899
     Region          2..839
                     /region_name="ligD"
                     /note="ATP-dependent DNA ligase; Reviewed; PRK05972"
                     /db_xref="CDD:235658"
     Region          7..162
                     /region_name="LigD_PE_dom"
                     /note="DNA ligase D, 3'-phosphoesterase domain; TIGR02777"
                     /db_xref="CDD:131824"
     Region          217..400
                     /region_name="Adenylation_DNA_ligase_LigD_LigC"
                     /note="Adenylation domain of Mycobacterium tuberculosis
                     LigD and LigC-like ATP-dependent DNA ligases; cd07906"
                     /db_xref="CDD:185715"
     Site            order(236..239,241..243,258,287,299,319,376,379,381,395,
                     397)
                     /site_type="active"
                     /db_xref="CDD:185715"
     Site            order(238,241..243,257..259,261,263,299..300,303..305,376,
                     397,399)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:185715"
     Region          403..520
                     /region_name="OBF_DNA_ligase_LigD"
                     /note="The Oligonucleotide/oligosaccharide binding
                     (OB)-fold domain of ATP-dependent DNA ligase LigD is a
                     DNA-binding module that is part of the catalytic core
                     unit; cd07971"
                     /db_xref="CDD:153440"
     Site            order(422,441..442,444..449,502,504..505,507)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:153440"
     Region          542..840
                     /region_name="LigD"
                     /note="Eukaryotic-type DNA primase [Replication,
                     recombination and repair]; COG3285"
                     /db_xref="CDD:225823"
     Site            order(604,651,669,671,704,707..708,710,759,762,768,
                     776..778)
                     /site_type="other"
                     /note="nucleotide binding site [chemical binding]"
                     /db_xref="CDD:240132"
     CDS             1..840
                     /locus_tag="PA2138"
                     /coded_by="NC_002516.2:2353086..2355608"
                     /note="'Product name confidence: class 3 (Function
                     proposed based on presence of conserved amino acid motif,
                     structural feature or limited sequence similarity to an
                     experimentally studied gene)'"
                     /transl_table=11
                     /db_xref="GeneID:880451"
CONTIG      join(WP_003113639.1:1..840)
ORIGIN      
        1 mpsskplaey arkrdfrqtp epsgrkprkd stgllrycvq khdasrlhyd frleldgtlk
       61 swavpkgpcl dpavkrlavq vedhpldyad fegsipqghy gagdvivwdr gawtplddpr
      121 eglekghlsf aldgeklsgr whlirtnlrg kqsqwflvka kdgearsldr fdvlkerpds
      181 vlsertllpr hgeaatpaar parrgksggk tpmpewiape laslveqppr gewayelkld
      241 gyrlmsried ghvrlltrng hdwterlphl ekalaglglq rswldgelvv ldeegrpdfq
      301 alqnafeegr genilyvlfd lpyhegedlr dvaleerrar leallegrde dplrfsatla
      361 edprdllasa cklglegvig krlgsayrsr rsndwiklkc qlrqefvivg ytepkgsrrh
      421 igalllglys pdeerrlrya gkvgsgftaa slkkvrerle plavrsspla kvpparetgs
      481 vqwvrpqqlc evsyaqmtrg giirqavfhg lredkparev tgerpagppp lrgarkasag
      541 asraatagvr ishpqrlidp siqasklela efharyadll lrdlrerpvs lvrgpdgigg
      601 elffqkhaar lkipgivqld paldpghppl lqirsaealv gavqmgsief htwnaslanl
      661 erpdrfvldl dpdpalpwkr mleatqlslt lldelglraf lktsggkgmh llvplerrhg
      721 wdevkdfaqa isqhlarlmp erfsavsgpr nrvgkifvdy lrnsrgastv aaysvrareg
      781 lpvsvpvfre eldslqganq wnlrslpqrl delagddpwa dyagtrqris aamrrqlgrg
//
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