GenomeNet

Database: RefSeq
Entry: NP_308029
LinkDB: NP_308029
Original site: NP_308029 
LOCUS       NP_308029                820 aa            linear   CON 12-FEB-2021
DEFINITION  aspartokinase I [Escherichia coli O157:H7 str. Sakai].
ACCESSION   NP_308029
VERSION     NP_308029.1
DBLINK      BioProject: PRJNA226
            BioSample: SAMN01911278
DBSOURCE    REFSEQ: accession NC_002695.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7 str. Sakai
  ORGANISM  Escherichia coli O157:H7 str. Sakai
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hayashi,T., Makino,K., Ohnishi,M., Kurokawa,K., Ishii,K.,
            Yokoyama,K., Han,C.G., Ohtsubo,E., Nakayama,K., Murata,T.,
            Tanaka,M., Tobe,T., Iida,T., Takami,H., Honda,T., Sasakawa,C.,
            Ogasawara,N., Yasunaga,T., Kuhara,S., Shiba,T., Hattori,M. and
            Shinagawa,H.
  TITLE     Complete genome sequence of enterohemorrhagic Escherichia coli
            O157:H7 and genomic comparison with a laboratory strain K-12
  JOURNAL   DNA Res. 8 (1), 11-22 (2001)
   PUBMED   11258796
  REMARK    Erratum:[DNA Res 2001 Apr 27;8(2):96]
REFERENCE   2
  AUTHORS   Yokoyama,K., Makino,K., Kubota,Y., Watanabe,M., Kimura,S.,
            Yutsudo,C.H., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I.,
            Abe,H., Yoh,M., Iida,T., Ohnishi,M., Hayashi,T., Yasunaga,T.,
            Honda,T., Sasakawa,C. and Shinagawa,H.
  TITLE     Complete nucleotide sequence of the prophage VT1-Sakai carrying the
            Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli
            O157:H7 strain derived from the Sakai outbreak
  JOURNAL   Gene 258 (1-2), 127-139 (2000)
   PUBMED   11111050
REFERENCE   3
  AUTHORS   Ohnishi,M., Murata,T., Nakayama,K., Kuhara,S., Hattori,M.,
            Kurokawa,K., Yasunaga,T., Yokoyama,K., Makino,K., Shinagawa,H. and
            Hayashi,T.
  TITLE     Comparative analysis of the whole set of rRNA operons between an
            enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an
            Escherichia coli K-12 strain MG1655
  JOURNAL   Syst. Appl. Microbiol. 23 (3), 315-324 (2000)
   PUBMED   11108008
REFERENCE   4
  AUTHORS   Makino,K., Yokoyama,K., Kubota,Y., Yutsudo,C.H., Kimura,S.,
            Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Iida,T.,
            Yamamoto,K., Onishi,M., Hayashi,T., Yasunaga,T., Honda,T.,
            Sasakawa,C. and Shinagawa,H.
  TITLE     Complete nucleotide sequence of the prophage VT2-Sakai carrying the
            verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7
            derived from the Sakai outbreak
  JOURNAL   Genes Genet. Syst. 74 (5), 227-239 (1999)
   PUBMED   10734605
REFERENCE   5  (residues 1 to 820)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2018) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   6  (residues 1 to 820)
  AUTHORS   Ogura,Y., Kurokawa,K., Hayashi,T. and Hattori,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2000) Contact:Yoshitoshi Ogura Kyushu University;
            31-1 Maidashi Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to BAB33425.
            On May 10, 2018 this sequence version replased gi: 47118301
            
            This update is obtained as follows: the Sakai genome was
            resequenced by illumina MiSeq and PacBio RS II sequencers and 78
            sites of single base error, 11 sites of insertion (10 one-base
            insertions and a 141-base insertion) and 16 sites of deletion (14
            one-base deletions, a three-base deletion and a six-base deletion)
            were corrected. Annotation was performed using DFAST
            auto-annotation system with manual curation.
            
            This work was done in collaboration with Tetsuya Hayashi, Makoto
            Ohnishi, Keisuke Nakayama (Miyazaki Medical College), Kozo Makino,
            Ken Kurokawa, Katsushi Yokoyama, Masashi Tanaka, Takeshi Honda,
            Teruo Yasunaga, Hideo Shinagawa (Osaka University), Takahiro Murata
            (Shinshu University), Chang-Gyun Han, Eiichi Ohtsubo, Toru Tobe,
            Chihiro Sasakawa (University of Tokyo), Hideto Takami (Japan Marine
            Science and Technology Center), Naotake Ogasawara (Nara Institute
            of Science and Technology), Satoru Kuhara (Kuyshu University), and
            supported by the Research for the Future Program of the Japan
            Society for the Promotion of Science.
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Escherichia coli O157:H7 str. Sakai"
                     /strain="Sakai"
                     /sub_strain="RIMD 0509952"
                     /serovar="O157:H7"
                     /db_xref="taxon:386585"
     Protein         1..820
                     /product="aspartokinase I"
                     /calculated_mol_wt=88949
     Region          1..820
                     /region_name="thrA"
                     /note="bifunctional aspartokinase I/homoserine
                     dehydrogenase I; Provisional; PRK09436"
                     /db_xref="CDD:181856"
     CDS             1..820
                     /gene="thrA"
                     /locus_tag="ECs_0002"
                     /coded_by="NC_002695.2:354..2816"
                     /transl_table=11
                     /db_xref="GeneID:913388"
CONTIG      join(WP_001264698.1:1..820)
ORIGIN      
        1 mrvlkfggts vanaerflrv adilesnarq gqvatvlsap akitnhlvam iektisgqda
       61 lpnisdaeri faelltglaa aqpgfplaql ktfvdqefaq ikhvlhgisl lgqcpdsina
      121 alicrgekms iaimagvlea rghnvtvidp vekllavghy lestvdiaes trriaasrip
      181 adhmvlmagf tagnekgelv vlgrngsdys aavlaaclra dcceiwtdvd gvytcdprqv
      241 pdarllksms yqeamelsyf gakvlhprti tpiaqfqipc likntgnpqa pgtligasrd
      301 edelpvkgis nlnnmamfsv sgpgmkgmvg maarvfaams rarisvvlit qssseysisf
      361 cvpqsdcvra eramqeefyl elkegllepl avterlaiis vvgdgmrtlr gisakffaal
      421 araninivai aqgssersis vvvnnddatt gvrvthqmlf ntdqvievfv igvggvggal
      481 leqlkrqqsw lknkhidlrv cgvanskall tnvhglnlen wqeelaqake pfnlgrlirl
      541 vkeyhllnpv ivdctssqav adqyadflre gfhvvtpnkk antssmdyyh llrhaaeksr
      601 rkflydtnvg aglpvienlq nllnagdelm kfsgilsgsl syifgkldeg msfseattla
      661 remgytepdp rddlsgmdva rkllilaret greleladie iepvlpaefn aegdvaafma
      721 nlsqlddlfa arvakardeg kvlryvgnid edgvcrvkia evdgndplfk vkngenalaf
      781 yshyyqplpl vlrgygagnd vtaagvfadl lrtlswklgv
//
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