GenomeNet

Database: RefSeq
Entry: NP_308063
LinkDB: NP_308063
Original site: NP_308063 
LOCUS       NP_308063               1073 aa            linear   CON 15-AUG-2018
DEFINITION  carbamoyl-phosphate synthase large subunit [Escherichia coli
            O157:H7 str. Sakai].
ACCESSION   NP_308063
VERSION     NP_308063.1
DBLINK      BioProject: PRJNA226
            BioSample: SAMN01911278
DBSOURCE    REFSEQ: accession NC_002695.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7 str. Sakai
  ORGANISM  Escherichia coli O157:H7 str. Sakai
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hayashi,T., Makino,K., Ohnishi,M., Kurokawa,K., Ishii,K.,
            Yokoyama,K., Han,C.G., Ohtsubo,E., Nakayama,K., Murata,T.,
            Tanaka,M., Tobe,T., Iida,T., Takami,H., Honda,T., Sasakawa,C.,
            Ogasawara,N., Yasunaga,T., Kuhara,S., Shiba,T., Hattori,M. and
            Shinagawa,H.
  TITLE     Complete genome sequence of enterohemorrhagic Escherichia coli
            O157:H7 and genomic comparison with a laboratory strain K-12
  JOURNAL   DNA Res. 8 (1), 11-22 (2001)
   PUBMED   11258796
  REMARK    Erratum:[DNA Res 2001 Apr 27;8(2):96]
REFERENCE   2
  AUTHORS   Yokoyama,K., Makino,K., Kubota,Y., Watanabe,M., Kimura,S.,
            Yutsudo,C.H., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I.,
            Abe,H., Yoh,M., Iida,T., Ohnishi,M., Hayashi,T., Yasunaga,T.,
            Honda,T., Sasakawa,C. and Shinagawa,H.
  TITLE     Complete nucleotide sequence of the prophage VT1-Sakai carrying the
            Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli
            O157:H7 strain derived from the Sakai outbreak
  JOURNAL   Gene 258 (1-2), 127-139 (2000)
   PUBMED   11111050
REFERENCE   3
  AUTHORS   Ohnishi,M., Murata,T., Nakayama,K., Kuhara,S., Hattori,M.,
            Kurokawa,K., Yasunaga,T., Yokoyama,K., Makino,K., Shinagawa,H. and
            Hayashi,T.
  TITLE     Comparative analysis of the whole set of rRNA operons between an
            enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an
            Escherichia coli K-12 strain MG1655
  JOURNAL   Syst. Appl. Microbiol. 23 (3), 315-324 (2000)
   PUBMED   11108008
REFERENCE   4
  AUTHORS   Makino,K., Yokoyama,K., Kubota,Y., Yutsudo,C.H., Kimura,S.,
            Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Iida,T.,
            Yamamoto,K., Onishi,M., Hayashi,T., Yasunaga,T., Honda,T.,
            Sasakawa,C. and Shinagawa,H.
  TITLE     Complete nucleotide sequence of the prophage VT2-Sakai carrying the
            verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7
            derived from the Sakai outbreak
  JOURNAL   Genes Genet. Syst. 74 (5), 227-239 (1999)
   PUBMED   10734605
REFERENCE   5  (residues 1 to 1073)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2018) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   6  (residues 1 to 1073)
  AUTHORS   Ogura,Y., Kurokawa,K., Hayashi,T. and Hattori,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2000) Contact:Yoshitoshi Ogura Kyushu University;
            31-1 Maidashi Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to BAB33459.
            On May 10, 2018 this sequence version replased gi: 47118301
            
            This update is obtained as follows: the Sakai genome was
            resequenced by illumina MiSeq and PacBio RS II sequencers and 78
            sites of single base error, 11 sites of insertion (10 one-base
            insertions and a 141-base insertion) and 16 sites of deletion (14
            one-base deletions, a three-base deletion and a six-base deletion)
            were corrected. Annotation was performed using DFAST
            auto-annotation system with manual curation.
            
            This work was done in collaboration with Tetsuya Hayashi, Makoto
            Ohnishi, Keisuke Nakayama (Miyazaki Medical College), Kozo Makino,
            Ken Kurokawa, Katsushi Yokoyama, Masashi Tanaka, Takeshi Honda,
            Teruo Yasunaga, Hideo Shinagawa (Osaka University), Takahiro Murata
            (Shinshu University), Chang-Gyun Han, Eiichi Ohtsubo, Toru Tobe,
            Chihiro Sasakawa (University of Tokyo), Hideto Takami (Japan Marine
            Science and Technology Center), Naotake Ogasawara (Nara Institute
            of Science and Technology), Satoru Kuhara (Kuyshu University), and
            supported by the Research for the Future Program of the Japan
            Society for the Promotion of Science.
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Escherichia coli O157:H7 str. Sakai"
                     /strain="Sakai"
                     /sub_strain="RIMD 0509952"
                     /serovar="O157:H7"
                     /db_xref="taxon:386585"
     Protein         1..1073
                     /product="carbamoyl-phosphate synthase large subunit"
                     /calculated_mol_wt=117725
     Region          1..1069
                     /region_name="carB"
                     /note="carbamoyl phosphate synthase large subunit;
                     Reviewed; PRK05294"
                     /db_xref="CDD:235393"
     CDS             1..1073
                     /gene="carB"
                     /locus_tag="ECs_0036"
                     /coded_by="NC_002695.2:35192..38413"
                     /transl_table=11
CONTIG      join(WP_001126371.1:1..1073)
ORIGIN      
        1 mpkrtdiksi lilgagpivi gqacefdysg aqackalree gyrvilvnsn patimtdpem
       61 adatyiepih wevvrkiiek erpdavlptm ggqtalncal elerqgvlee fgvtmigata
      121 daidkaedrr rfdvamkkig letarsgiah tmeealavaa dvgfpciirp sftmggsggg
      181 iaynreefee icargldlsp tkellidesl igwkeyemev vrdkndncii vcsienfdam
      241 gihtgdsitv apaqtltdke yqimrnasma vlreigvetg gsnvqfavnp kngrliviem
      301 nprvsrssal askatgfpia kvaaklavgy tldelmndit ggrtpasfep sidyvvtkip
      361 rfnfekfaga ndrlttqmks vgevmaigrt qqeslqkalr glevgatgfd pkvslddpea
      421 ltkirrelkd agaeriwyia dafraglsvd gvfnltnidr wflvqieelv rleekvaevg
      481 itglnaeflr qlkrkgfada rlaklagvre aeirklrdqy dlhpvykrvd tcaaefatdt
      541 aymystyeee ceanpstdre kimvlgggpn rigqgiefdy ccvhaslalr edgyetimvn
      601 cnpetvstdy dtsdrlyfep vtledvleiv riekpkgviv qyggqtplkl araleaagvp
      661 vigtspdaid raedrerfqh avdrlklkqp anatvtaiem avekakeigy plvvrpsyvl
      721 ggrameivyd eadlrryfqt avsvsndapv lldhflddav evdvdaicdg emvliggime
      781 hieqagvhsg dsacslpayt lsqeiqdvmr qqvqklafel qvrglmnvqf avknnevyli
      841 evnpraartv pfvskatgvp lakvaarvma gkslaeqgvt kevippyysv kevvlpfnkf
      901 pgvdpllgpe mrstgevmgv grtfaeafak aqlgsnstmk khgrallsvr egdkervvdl
      961 aakllkqgfe ldathgtaiv lgeaginprl vnkvhegrph iqdrikngey tyiinttsgr
     1021 raiedsrvir rsalqykvhy dttlnggfat amalnadate kvisvqemha qik
//
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