LOCUS NP_775150 247 aa linear ROD 28-APR-2025
DEFINITION cationic trypsin-3 precursor [Rattus norvegicus].
ACCESSION NP_775150
VERSION NP_775150.1
DBSOURCE REFSEQ: accession NM_173127.1
KEYWORDS RefSeq; RefSeq Select.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (residues 1 to 247)
AUTHORS Jiang,C., Zheng,H., Liu,S. and Fang,K.
TITLE Relationship between carbachol hyperstimulation-induced pancreatic
intracellular trypsinogen and NF-kappa B activation in rats in
vitro
JOURNAL J Huazhong Univ Sci Technolog Med Sci 28 (1), 69-72 (2008)
PUBMED 18278461
REMARK GeneRIF: NF-kappa B activation is likely not necessary for high
concentrations of carbachol-induced trypsinogen activation in
pancreatic acinar cells in vitro
REFERENCE 2 (residues 1 to 247)
AUTHORS Gaiser,S., Ahler,A., Gundling,F., Kruse,M.L., Savkovic,V.,
Selig,L., Teich,N., Tomasini,R., Dagorn,J.C., Mossner,J., Keim,V.
and Bodeker,H.
TITLE Expression of mutated cationic trypsinogen reduces cellular
viability in AR4-2J cells
JOURNAL Biochem Biophys Res Commun 334 (2), 721-728 (2005)
PUBMED 16036133
REMARK GeneRIF: Expression of trypsin and R122H trypsinogen induced
caspase-3 activity. Acinar cells might react to intracellular
trypsin activity by triggering apoptosis.
REFERENCE 3 (residues 1 to 247)
AUTHORS Wang,S., He,R. and Anderson,R.
TITLE PrM- and cell-binding domains of the dengue virus E protein
JOURNAL J Virol 73 (3), 2547-2551 (1999)
PUBMED 9971841
REFERENCE 4 (residues 1 to 247)
AUTHORS Roach,J.C., Wang,K., Gan,L. and Hood,L.
TITLE The molecular evolution of the vertebrate trypsinogens
JOURNAL J Mol Evol 45 (6), 640-652 (1997)
PUBMED 9419241
REMARK GeneRIF: This trypsinogen is typical of non-human gnathostomata
trypinsogens; it has 6 predicted cystine bridges and an acidic
activation peptide. It clusters with group II ('cationic')
trypsins.
REFERENCE 5 (residues 1 to 247)
AUTHORS Fletcher,T.S., Alhadeff,M., Craik,C.S. and Largman,C.
TITLE Isolation and characterization of a cDNA encoding rat cationic
trypsinogen
JOURNAL Biochemistry 26 (11), 3081-3086 (1987)
PUBMED 3607011
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence was derived from M16624.1.
##Evidence-Data-START##
Transcript exon combination :: M16624.1, FQ226997.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns
SAMEA5756307 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
RefSeq Select criteria :: based on single protein-coding transcript
##RefSeq-Attributes-END##
FEATURES Location/Qualifiers
source 1..247
/organism="Rattus norvegicus"
/db_xref="taxon:10116"
/chromosome="4"
/map="4q23"
Protein 1..247
/product="cationic trypsin-3 precursor"
/EC_number="3.4.21.4"
/note="cationic trypsin-3; pretrypsinogen III; cationic
trypsin III; cationic trypsinogen; protease, serine, 3"
/calculated_mol_wt=24751
sig_peptide 1..15
/inference="COORDINATES: ab initio prediction:SignalP:6.0"
/calculated_mol_wt=1536
mat_peptide 24..247
/product="cationic trypsin-3"
/calculated_mol_wt=23852
Region 25..243
/region_name="Tryp_SPc"
/note="Trypsin-like serine protease; Many of these are
synthesized as inactive precursor zymogens that are
cleaved during limited proteolysis to generate their
active forms. Alignment contains also inactive enzymes
that have substitutions of the catalytic triad...;
cd00190"
/db_xref="CDD:238113"
Site 25
/site_type="active"
/note="cleavage site [active]"
/db_xref="CDD:238113"
Site order(64,108,201)
/site_type="active"
/db_xref="CDD:238113"
Site order(195,216,218)
/site_type="other"
/note="substrate binding sites [chemical binding]"
/db_xref="CDD:238113"
Site 195
/site_type="other"
/note="Required for specificity. /evidence=ECO:0000250;
propagated from UniProtKB/Swiss-Prot (P08426.1)"
CDS 1..247
/gene="Prss3b"
/gene_synonym="Prss3; Try3"
/coded_by="NM_173127.1:1..744"
/db_xref="GeneID:286911"
/db_xref="RGD:708437"
ORIGIN
1 mkaliflafl gaavalpldd dddkivggyt cqknslpyqv slnagyhfcg gslinsqwvv
61 saahcyksri qvrlgehnid vveggeqfid aakiirhpsy nantfdndim liklnspatl
121 nsrvstvslp rscgssgtkc lvsgwgntls sgtnypsllq cldapvlsds sckssypgki
181 tsnmfclgfl eggkdscqgd sggpvvcngq lqgvvswgyg caqkgkpgvy tkvcnyvnwi
241 qqtvaan
//