GenomeNet

Database: RefSeq
Entry: NP_857593
LinkDB: NP_857593
Original site: NP_857593 
LOCUS       NP_857593                529 aa            linear   PRI 08-JUL-2023
DEFINITION  kunitz-type protease inhibitor 1 isoform 1 precursor [Homo
            sapiens].
ACCESSION   NP_857593
VERSION     NP_857593.1
DBSOURCE    REFSEQ: accession NM_181642.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 529)
  AUTHORS   Lu DD, Huang N, Li SA, Fang JR, Lai CH, Wang JK, Chan KS, Johnson
            MD and Lin CY.
  TITLE     HAI-1 is required for the novel role of FGFBP1 in maintenance of
            cell morphology and F-actin rearrangement in human keratinocytes
  JOURNAL   Hum Cell 36 (4), 1403-1415 (2023)
   PUBMED   37076641
  REMARK    GeneRIF: HAI-1 is required for the novel role of FGFBP1 in
            maintenance of cell morphology and F-actin rearrangement in human
            keratinocytes.
REFERENCE   2  (residues 1 to 529)
  AUTHORS   Izumi A, Yamamoto K, Kawaguchi M, Yamashita F, Fukushima T, Kiwaki
            T, Tanaka H, Yamashita Y and Kataoka H.
  TITLE     Insufficiency of hepatocyte growth factor activator inhibitor-1
            confers lymphatic invasion of tongue carcinoma cells
  JOURNAL   Cancer Sci 113 (6), 2179-2193 (2022)
   PUBMED   35332604
  REMARK    GeneRIF: Insufficiency of hepatocyte growth factor activator
            inhibitor-1 confers lymphatic invasion of tongue carcinoma cells.
REFERENCE   3  (residues 1 to 529)
  AUTHORS   Yamashita F, Kaieda T, Shimomura T, Kawaguchi M, Lin CY, Johnson
            MD, Tanaka H, Kiwaki T, Fukushima T and Kataoka H.
  TITLE     Role of the polycystic kidney disease domain in matriptase
            chaperone activity and localization of hepatocyte growth factor
            activator inhibitor-1
  JOURNAL   FEBS J 289 (12), 3422-3439 (2022)
   PUBMED   35020274
  REMARK    GeneRIF: Role of the polycystic kidney disease domain in matriptase
            chaperone activity and localization of hepatocyte growth factor
            activator inhibitor-1.
            Review article
REFERENCE   4  (residues 1 to 529)
  AUTHORS   Wu Q, Yin G, Luo J, Zhang Y, Ai T, Tian J, Jin Y, Lei J and Liu S.
  TITLE     Comprehensive Analysis of the Expression and Prognostic Value of
            SPINT1/2 in Breast Carcinoma
  JOURNAL   Front Endocrinol (Lausanne) 12, 665666 (2021)
   PUBMED   34381422
  REMARK    GeneRIF: Comprehensive Analysis of the Expression and Prognostic
            Value of SPINT1/2 in Breast Carcinoma.
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 529)
  AUTHORS   Borowicz S, Principe DR, Dorman MJ, McHenry AJ, Sondarva G, Kumar
            S, Ananthanarayanan V, Simms PE, Hess A and Rana A.
  TITLE     HAI-1 is an independent predictor of lung cancer mortality and is
            required for M1 macrophage polarization
  JOURNAL   PLoS One 16 (6), e0252197 (2021)
   PUBMED   34185790
  REMARK    GeneRIF: HAI-1 is an independent predictor of lung cancer mortality
            and is required for M1 macrophage polarization.
            Publication Status: Online-Only
REFERENCE   6  (residues 1 to 529)
  AUTHORS   Shimomura T, Denda K, Kawaguchi T, Matsumoto K, Miyazawa K and
            Kitamura N.
  TITLE     Multiple sites of proteolytic cleavage to release soluble forms of
            hepatocyte growth factor activator inhibitor type 1 from a
            transmembrane form
  JOURNAL   J Biochem 126 (5), 821-828 (1999)
   PUBMED   10544273
REFERENCE   7  (residues 1 to 529)
  AUTHORS   Lin CY, Anders J, Johnson M and Dickson RB.
  TITLE     Purification and characterization of a complex containing
            matriptase and a Kunitz-type serine protease inhibitor from human
            milk
  JOURNAL   J Biol Chem 274 (26), 18237-18242 (1999)
   PUBMED   10373425
REFERENCE   8  (residues 1 to 529)
  AUTHORS   Kataoka H, Suganuma T, Shimomura T, Itoh H, Kitamura N, Nabeshima K
            and Koono M.
  TITLE     Distribution of hepatocyte growth factor activator inhibitor type 1
            (HAI-1) in human tissues. Cellular surface localization of HAI-1 in
            simple columnar epithelium and its modulated expression in injured
            and regenerative tissues
  JOURNAL   J Histochem Cytochem 47 (5), 673-682 (1999)
   PUBMED   10219059
REFERENCE   9  (residues 1 to 529)
  AUTHORS   Yamada T, Tsujioka Y, Taguchi J, Takahashi M, Tsuboi Y and
            Shimomura T.
  TITLE     White matter astrocytes produce hepatocyte growth factor activator
            inhibitor in human brain tissues
  JOURNAL   Exp Neurol 153 (1), 60-64 (1998)
   PUBMED   9743567
REFERENCE   10 (residues 1 to 529)
  AUTHORS   Shimomura T, Denda K, Kitamura A, Kawaguchi T, Kito M, Kondo J,
            Kagaya S, Qin L, Takata H, Miyazawa K and Kitamura N.
  TITLE     Hepatocyte growth factor activator inhibitor, a novel Kunitz-type
            serine protease inhibitor
  JOURNAL   J Biol Chem 272 (10), 6370-6376 (1997)
   PUBMED   9045658
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM763214.1, AY358969.1,
            AC012476.8 and AI684245.1.
            
            Summary: The protein encoded by this gene is a member of the Kunitz
            family of serine protease inhibitors. The protein is a potent
            inhibitor specific for HGF activator and is thought to be involved
            in the regulation of the proteolytic activation of HGF in injured
            tissues. Alternative splicing results in multiple variants encoding
            different isoforms. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1), also known as HAI-1B, encodes
            the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.257274.1,
                                           SRR1803611.193757.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.1"
     Protein         1..529
                     /product="kunitz-type protease inhibitor 1 isoform 1
                     precursor"
                     /note="serine protease inhibitor, Kunitz type 1;
                     Kunitz-type protease inhibitor 1; HAI-1; hepatocyte growth
                     factor activator inhibitor type 1"
                     /calculated_mol_wt=54825
     sig_peptide     1..35
                     /inference="COORDINATES: ab initio prediction:SignalP:4.0"
                     /calculated_mol_wt=3591
     mat_peptide     36..529
                     /product="kunitz-type protease inhibitor 1 isoform 1"
                     /calculated_mol_wt=54825
     Region          50..139
                     /region_name="MANEC"
                     /note="MANEC domain; pfam07502"
                     /db_xref="CDD:429497"
     Site            66
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O43278.2)"
     Site            235
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O43278.2)"
     Region          245..303
                     /region_name="Kunitz_HAI1_1-like"
                     /note="Kunitz domain 1 of hepatocyte growth factor
                     activator inhibitor-1 (HAI-1); cd22623"
                     /db_xref="CDD:438666"
     Site            order(256..265,279,281,283..284,286,289,291)
                     /site_type="other"
                     /note="serine protease binding site [polypeptide binding]"
                     /db_xref="CDD:438666"
     Site            260..261
                     /site_type="other"
                     /note="Reactive bond. /evidence=ECO:0000250; propagated
                     from UniProtKB/Swiss-Prot (O43278.2)"
     Region          334..366
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(340,348,359..360)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(352,355,359,365..366)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            362..366
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          390..450
                     /region_name="Kunitz_HAI1_2-like"
                     /note="Kunitz domain 2 of hepatocyte growth factor
                     activator inhibitor-1 (HAI1); cd22624"
                     /db_xref="CDD:438667"
     Site            order(397..401,403..405,420,424)
                     /site_type="other"
                     /note="putative serine protease binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438667"
     Site            401..402
                     /site_type="other"
                     /note="Reactive bond. /evidence=ECO:0000250; propagated
                     from UniProtKB/Swiss-Prot (O43278.2)"
     Site            523
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O43278.2)"
     CDS             1..529
                     /gene="SPINT1"
                     /gene_synonym="HAI; HAI1; MANSC2"
                     /coded_by="NM_181642.3:205..1794"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /db_xref="CCDS:CCDS10067.1"
                     /db_xref="GeneID:6692"
                     /db_xref="HGNC:HGNC:11246"
                     /db_xref="MIM:605123"
ORIGIN      
        1 mapartmara rlapagipav alwllctlgl qgtqagpppa ppglpagadc lnsftagvpg
       61 fvldtnasvs ngatflespt vrrgwdcvra ccttqncnla lvelqpdrge daiaacflin
      121 clyeqnfvck fapregfiny ltrevyrsyr qlrtqgfggs gipkawagid lkvqpqeplv
      181 lkdventdwr llrgdtdvrv erkdpnqvel wglkegtylf qltvtssdhp edtanvtvtv
      241 lstkqtedyc lasnkvgrcr gsfprwyydp teqicksfvy ggclgnknny lreeecilac
      301 rgvqggplrg ssgaqatfpq gpsmerrhpv csgtcqptqf rcsngccids flecddtpnc
      361 pdasdeaace kytsgfdelq rihfpsdkgh cvdlpdtglc kesiprwyyn pfsehcarft
      421 yggcygnknn feeeqqcles crgiskkdvf glrreipips tgsvemavav flvicivvvv
      481 ailgycffkn qrkdfhghhh hppptpasst vsttedtehl vynhttrpl
//
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