LOCUS NP_857593 529 aa linear PRI 08-JUL-2023
DEFINITION kunitz-type protease inhibitor 1 isoform 1 precursor [Homo
sapiens].
ACCESSION NP_857593
VERSION NP_857593.1
DBSOURCE REFSEQ: accession NM_181642.3
KEYWORDS RefSeq.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (residues 1 to 529)
AUTHORS Lu DD, Huang N, Li SA, Fang JR, Lai CH, Wang JK, Chan KS, Johnson
MD and Lin CY.
TITLE HAI-1 is required for the novel role of FGFBP1 in maintenance of
cell morphology and F-actin rearrangement in human keratinocytes
JOURNAL Hum Cell 36 (4), 1403-1415 (2023)
PUBMED 37076641
REMARK GeneRIF: HAI-1 is required for the novel role of FGFBP1 in
maintenance of cell morphology and F-actin rearrangement in human
keratinocytes.
REFERENCE 2 (residues 1 to 529)
AUTHORS Izumi A, Yamamoto K, Kawaguchi M, Yamashita F, Fukushima T, Kiwaki
T, Tanaka H, Yamashita Y and Kataoka H.
TITLE Insufficiency of hepatocyte growth factor activator inhibitor-1
confers lymphatic invasion of tongue carcinoma cells
JOURNAL Cancer Sci 113 (6), 2179-2193 (2022)
PUBMED 35332604
REMARK GeneRIF: Insufficiency of hepatocyte growth factor activator
inhibitor-1 confers lymphatic invasion of tongue carcinoma cells.
REFERENCE 3 (residues 1 to 529)
AUTHORS Yamashita F, Kaieda T, Shimomura T, Kawaguchi M, Lin CY, Johnson
MD, Tanaka H, Kiwaki T, Fukushima T and Kataoka H.
TITLE Role of the polycystic kidney disease domain in matriptase
chaperone activity and localization of hepatocyte growth factor
activator inhibitor-1
JOURNAL FEBS J 289 (12), 3422-3439 (2022)
PUBMED 35020274
REMARK GeneRIF: Role of the polycystic kidney disease domain in matriptase
chaperone activity and localization of hepatocyte growth factor
activator inhibitor-1.
Review article
REFERENCE 4 (residues 1 to 529)
AUTHORS Wu Q, Yin G, Luo J, Zhang Y, Ai T, Tian J, Jin Y, Lei J and Liu S.
TITLE Comprehensive Analysis of the Expression and Prognostic Value of
SPINT1/2 in Breast Carcinoma
JOURNAL Front Endocrinol (Lausanne) 12, 665666 (2021)
PUBMED 34381422
REMARK GeneRIF: Comprehensive Analysis of the Expression and Prognostic
Value of SPINT1/2 in Breast Carcinoma.
Publication Status: Online-Only
REFERENCE 5 (residues 1 to 529)
AUTHORS Borowicz S, Principe DR, Dorman MJ, McHenry AJ, Sondarva G, Kumar
S, Ananthanarayanan V, Simms PE, Hess A and Rana A.
TITLE HAI-1 is an independent predictor of lung cancer mortality and is
required for M1 macrophage polarization
JOURNAL PLoS One 16 (6), e0252197 (2021)
PUBMED 34185790
REMARK GeneRIF: HAI-1 is an independent predictor of lung cancer mortality
and is required for M1 macrophage polarization.
Publication Status: Online-Only
REFERENCE 6 (residues 1 to 529)
AUTHORS Shimomura T, Denda K, Kawaguchi T, Matsumoto K, Miyazawa K and
Kitamura N.
TITLE Multiple sites of proteolytic cleavage to release soluble forms of
hepatocyte growth factor activator inhibitor type 1 from a
transmembrane form
JOURNAL J Biochem 126 (5), 821-828 (1999)
PUBMED 10544273
REFERENCE 7 (residues 1 to 529)
AUTHORS Lin CY, Anders J, Johnson M and Dickson RB.
TITLE Purification and characterization of a complex containing
matriptase and a Kunitz-type serine protease inhibitor from human
milk
JOURNAL J Biol Chem 274 (26), 18237-18242 (1999)
PUBMED 10373425
REFERENCE 8 (residues 1 to 529)
AUTHORS Kataoka H, Suganuma T, Shimomura T, Itoh H, Kitamura N, Nabeshima K
and Koono M.
TITLE Distribution of hepatocyte growth factor activator inhibitor type 1
(HAI-1) in human tissues. Cellular surface localization of HAI-1 in
simple columnar epithelium and its modulated expression in injured
and regenerative tissues
JOURNAL J Histochem Cytochem 47 (5), 673-682 (1999)
PUBMED 10219059
REFERENCE 9 (residues 1 to 529)
AUTHORS Yamada T, Tsujioka Y, Taguchi J, Takahashi M, Tsuboi Y and
Shimomura T.
TITLE White matter astrocytes produce hepatocyte growth factor activator
inhibitor in human brain tissues
JOURNAL Exp Neurol 153 (1), 60-64 (1998)
PUBMED 9743567
REFERENCE 10 (residues 1 to 529)
AUTHORS Shimomura T, Denda K, Kitamura A, Kawaguchi T, Kito M, Kondo J,
Kagaya S, Qin L, Takata H, Miyazawa K and Kitamura N.
TITLE Hepatocyte growth factor activator inhibitor, a novel Kunitz-type
serine protease inhibitor
JOURNAL J Biol Chem 272 (10), 6370-6376 (1997)
PUBMED 9045658
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from BM763214.1, AY358969.1,
AC012476.8 and AI684245.1.
Summary: The protein encoded by this gene is a member of the Kunitz
family of serine protease inhibitors. The protein is a potent
inhibitor specific for HGF activator and is thought to be involved
in the regulation of the proteolytic activation of HGF in injured
tissues. Alternative splicing results in multiple variants encoding
different isoforms. [provided by RefSeq, Jul 2008].
Transcript Variant: This variant (1), also known as HAI-1B, encodes
the longer isoform (1).
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR1660805.257274.1,
SRR1803611.193757.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns
SAMEA1968189, SAMEA1968540
[ECO:0000348]
##Evidence-Data-END##
FEATURES Location/Qualifiers
source 1..529
/organism="Homo sapiens"
/db_xref="taxon:9606"
/chromosome="15"
/map="15q15.1"
Protein 1..529
/product="kunitz-type protease inhibitor 1 isoform 1
precursor"
/note="serine protease inhibitor, Kunitz type 1;
Kunitz-type protease inhibitor 1; HAI-1; hepatocyte growth
factor activator inhibitor type 1"
/calculated_mol_wt=54825
sig_peptide 1..35
/inference="COORDINATES: ab initio prediction:SignalP:4.0"
/calculated_mol_wt=3591
mat_peptide 36..529
/product="kunitz-type protease inhibitor 1 isoform 1"
/calculated_mol_wt=54825
Region 50..139
/region_name="MANEC"
/note="MANEC domain; pfam07502"
/db_xref="CDD:429497"
Site 66
/site_type="glycosylation"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255; propagated from
UniProtKB/Swiss-Prot (O43278.2)"
Site 235
/site_type="glycosylation"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255; propagated from
UniProtKB/Swiss-Prot (O43278.2)"
Region 245..303
/region_name="Kunitz_HAI1_1-like"
/note="Kunitz domain 1 of hepatocyte growth factor
activator inhibitor-1 (HAI-1); cd22623"
/db_xref="CDD:438666"
Site order(256..265,279,281,283..284,286,289,291)
/site_type="other"
/note="serine protease binding site [polypeptide binding]"
/db_xref="CDD:438666"
Site 260..261
/site_type="other"
/note="Reactive bond. /evidence=ECO:0000250; propagated
from UniProtKB/Swiss-Prot (O43278.2)"
Region 334..366
/region_name="LDLa"
/note="Low-density lipoprotein receptor domain class A;
smart00192"
/db_xref="CDD:197566"
Site order(340,348,359..360)
/site_type="active"
/note="putative binding surface [active]"
/db_xref="CDD:238060"
Site order(352,355,359,365..366)
/site_type="other"
/note="calcium-binding site [ion binding]"
/db_xref="CDD:238060"
Site 362..366
/site_type="other"
/note="D-X-S-D-E motif"
/db_xref="CDD:238060"
Region 390..450
/region_name="Kunitz_HAI1_2-like"
/note="Kunitz domain 2 of hepatocyte growth factor
activator inhibitor-1 (HAI1); cd22624"
/db_xref="CDD:438667"
Site order(397..401,403..405,420,424)
/site_type="other"
/note="putative serine protease binding site [polypeptide
binding]"
/db_xref="CDD:438667"
Site 401..402
/site_type="other"
/note="Reactive bond. /evidence=ECO:0000250; propagated
from UniProtKB/Swiss-Prot (O43278.2)"
Site 523
/site_type="glycosylation"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255; propagated from
UniProtKB/Swiss-Prot (O43278.2)"
CDS 1..529
/gene="SPINT1"
/gene_synonym="HAI; HAI1; MANSC2"
/coded_by="NM_181642.3:205..1794"
/note="isoform 1 precursor is encoded by transcript
variant 1"
/db_xref="CCDS:CCDS10067.1"
/db_xref="GeneID:6692"
/db_xref="HGNC:HGNC:11246"
/db_xref="MIM:605123"
ORIGIN
1 mapartmara rlapagipav alwllctlgl qgtqagpppa ppglpagadc lnsftagvpg
61 fvldtnasvs ngatflespt vrrgwdcvra ccttqncnla lvelqpdrge daiaacflin
121 clyeqnfvck fapregfiny ltrevyrsyr qlrtqgfggs gipkawagid lkvqpqeplv
181 lkdventdwr llrgdtdvrv erkdpnqvel wglkegtylf qltvtssdhp edtanvtvtv
241 lstkqtedyc lasnkvgrcr gsfprwyydp teqicksfvy ggclgnknny lreeecilac
301 rgvqggplrg ssgaqatfpq gpsmerrhpv csgtcqptqf rcsngccids flecddtpnc
361 pdasdeaace kytsgfdelq rihfpsdkgh cvdlpdtglc kesiprwyyn pfsehcarft
421 yggcygnknn feeeqqcles crgiskkdvf glrreipips tgsvemavav flvicivvvv
481 ailgycffkn qrkdfhghhh hppptpasst vsttedtehl vynhttrpl
//