GenomeNet

Database: RefSeq
Entry: NP_999215
LinkDB: NP_999215
Original site: NP_999215 
LOCUS       NP_999215                241 aa            linear   MAM 10-NOV-2018
DEFINITION  glutathione S-transferase omega-1 [Sus scrofa].
ACCESSION   NP_999215
VERSION     NP_999215.1
DBSOURCE    REFSEQ: accession NM_214050.2
KEYWORDS    RefSeq.
SOURCE      Sus scrofa (pig)
  ORGANISM  Sus scrofa
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae;
            Sus.
REFERENCE   1  (residues 1 to 241)
  AUTHORS   Uenishi H, Eguchi-Ogawa T, Shinkai H, Okumura N, Suzuki K, Toki D,
            Hamasima N and Awata T.
  TITLE     PEDE (Pig EST Data Explorer) has been expanded into Pig Expression
            Data Explorer, including 10 147 porcine full-length cDNA sequences
  JOURNAL   Nucleic Acids Res. 35 (Database issue), D650-D653 (2007)
   PUBMED   17145712
REFERENCE   2  (residues 1 to 241)
  AUTHORS   Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H,
            Okumura N, Hamasima N and Awata T.
  TITLE     PEDE (Pig EST Data Explorer): construction of a database for ESTs
            derived from porcine full-length cDNA libraries
  JOURNAL   Nucleic Acids Res. 32 (Database issue), D484-D488 (2004)
   PUBMED   14681463
REFERENCE   3  (residues 1 to 241)
  AUTHORS   Rouimi P, Anglade P, Benzekri A, Costet P, Debrauwer L, Pineau T
            and Tulliez J.
  TITLE     Purification and characterization of a glutathione S-transferase
            Omega in pig: evidence for two distinct organ-specific transcripts
  JOURNAL   Biochem. J. 358 (Pt 1), 257-262 (2001)
   PUBMED   11485575
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF188838.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF188838.3, AK391766.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1876824, SAMEA1876828
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Sus scrofa"
                     /db_xref="taxon:9823"
                     /chromosome="14"
                     /map="14"
     Protein         1..241
                     /product="glutathione S-transferase omega-1"
                     /EC_number="2.5.1.18"
                     /EC_number="1.8.5.1"
                     /EC_number="1.20.4.2"
                     /note="GSTO-1; glutathione-dependent dehydroascorbate
                     reductase; MMA(V) reductase; monomethylarsonic acid
                     reductase; S-(Phenacyl)glutathione reductase; glutathione
                     S-transferase omega 1-1"
                     /calculated_mol_wt=27288
     Site            2
                     /site_type="other"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N-acetylserine. {ECO:0000250|UniProtKB:P78417};
                     propagated from UniProtKB/Swiss-Prot (Q9N1F5.2)"
     Region          5..94
                     /region_name="GST_N_Omega"
                     /note="GST_N family, Class Omega subfamily; GSTs are
                     cytosolic dimeric proteins involved in cellular
                     detoxification by catalyzing the conjugation of
                     glutathione (GSH) with a wide range of endogenous and
                     xenobiotic alkylating agents, including carcinogens;
                     cd03055"
                     /db_xref="CDD:239353"
     Site            order(7..8,29..31,33,36..37,39..41,43..44,55,57)
                     /site_type="other"
                     /note="C-terminal domain interface [polypeptide binding]"
                     /db_xref="CDD:239353"
     Region          26..225
                     /region_name="GstA"
                     /note="Glutathione S-transferase [Posttranslational
                     modification, protein turnover, chaperones]; COG0625"
                     /db_xref="CDD:223698"
     Site            order(32,34,59,71..73,85..86)
                     /site_type="other"
                     /note="GSH binding site (G-site) [chemical binding]"
                     /db_xref="CDD:239353"
     Site            32
                     /site_type="active"
                     /note="active site cysteine [active]"
                     /db_xref="CDD:239353"
     Site            57
                     /site_type="other"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine. {ECO:0000250|UniProtKB:P78417};
                     propagated from UniProtKB/Swiss-Prot (Q9N1F5.2)"
     Site            order(71,84..85,87..88,91)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:239353"
     Region          85..86
                     /region_name="Glutathione binding.
                     {ECO:0000250|UniProtKB:P78417}"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9N1F5.2)"
     Region          108..229
                     /region_name="GST_C_Omega"
                     /note="C-terminal, alpha helical domain of Class Omega
                     Glutathione S-transferases; cd03184"
                     /db_xref="CDD:198293"
     Site            order(114..115,118..119,122,152)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:198293"
     Site            order(121,124..125,128..129,180,183,222)
                     /site_type="other"
                     /note="substrate binding pocket (H-site) [chemical
                     binding]"
                     /db_xref="CDD:198293"
     Site            143
                     /site_type="other"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine. {ECO:0000250|UniProtKB:P78417};
                     propagated from UniProtKB/Swiss-Prot (Q9N1F5.2)"
     Site            148
                     /site_type="other"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine. {ECO:0000250|UniProtKB:P78417};
                     propagated from UniProtKB/Swiss-Prot (Q9N1F5.2)"
     Site            152
                     /site_type="other"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine. {ECO:0000250|UniProtKB:P78417};
                     propagated from UniProtKB/Swiss-Prot (Q9N1F5.2)"
     Site            order(172,175..176,179,182..183,211,215,225,229)
                     /site_type="other"
                     /note="N-terminal domain interface [polypeptide binding]"
                     /db_xref="CDD:198293"
     CDS             1..241
                     /gene="GSTO1"
                     /gene_synonym="GSTO 1-1; SPG-R"
                     /coded_by="NM_214050.2:73..798"
                     /db_xref="GeneID:397117"
ORIGIN      
        1 msggsarslg kgsappgpvp eglirvysmr fcpfaqrtll vlnakgirhq vininlknkp
       61 ewffqknpsg lvpvlensqg qliyesaitc eyldeaypgk kllpddpyek acqkmvfels
      121 skvppllirf irreneadcs glkeelrkef skleevltkk kttyfggssl smidyliwpw
      181 ferlealeln ecidhtpklk lwmaammkdp avsalhiepr dlrafndlyl qnspeacdyg
      241 l
//
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