LOCUS       XP_018637240             929 aa            linear   INV 20-OCT-2016
DEFINITION  pyruvate dehydrogenase complex subunit PDH-E2 [Toxoplasma gondii
            ME49].
ACCESSION   XP_018637240
VERSION     XP_018637240.1
DBLINK      BioProject: PRJNA32719
            BioSample: SAMN00255192
DBSOURCE    REFSEQ: accession XM_018779368.1
KEYWORDS    RefSeq.
SOURCE      Toxoplasma gondii ME49
  ORGANISM  Toxoplasma gondii ME49
            Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
            Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma.
REFERENCE   1  (residues 1 to 929)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2016) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   2  (residues 1 to 929)
  AUTHORS   Sibley,D., Venepally,P., Karamycheva,S., Hadjithomas,M., Khan,A.,
            Brunk,B., Roos,D., Caler,E. and Lorenzi,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2013) J. Craig Venter Institute, 9704 Medical
            Center Dr, Rockville, MD 20850, USA
  REMARK    ABPA02000001 and ABPA02000002 sequences updated by submitter
REFERENCE   3  (residues 1 to 929)
  AUTHORS   Sibley,D., Venepally,P., Karamycheva,S., Hadjithomas,M., Khan,A.,
            Brunk,B., Roos,D., Caler,E. and Lorenzi,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2012) J. Craig Venter Institute, 9704 Medical
            Center Dr, Rockville, MD 20850, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to EPT29904.
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Toxoplasma gondii ME49"
                     /strain="ME49"
                     /db_xref="taxon:508771"
                     /chromosome="VIIa"
     Protein         1..929
                     /product="pyruvate dehydrogenase complex subunit PDH-E2"
                     /EC_number="2.3.1.12"
                     /calculated_mol_wt=96749
     Region          135..208
                     /region_name="lipoyl_domain"
                     /note="Lipoyl domain of the dihydrolipoyl acyltransferase
                     component (E2) of 2-oxo acid dehydrogenases. 2-oxo acid
                     dehydrogenase multienzyme complexes, like pyruvate
                     dehydrogenase (PDH), 2-oxoglutarate dehydrogenase (OGDH)
                     and branched-chain 2-oxo acid...; cd06849"
                     /db_xref="CDD:133458"
     Site            order(165,172..177,181)
                     /site_type="active"
                     /note="E3 interaction surface [active]"
                     /db_xref="CDD:133458"
     Site            175
                     /site_type="other"
                     /note="lipoyl attachment site [posttranslational
                     modification]"
                     /db_xref="CDD:133458"
     Region          241..314
                     /region_name="lipoyl_domain"
                     /note="Lipoyl domain of the dihydrolipoyl acyltransferase
                     component (E2) of 2-oxo acid dehydrogenases. 2-oxo acid
                     dehydrogenase multienzyme complexes, like pyruvate
                     dehydrogenase (PDH), 2-oxoglutarate dehydrogenase (OGDH)
                     and branched-chain 2-oxo acid...; cd06849"
                     /db_xref="CDD:133458"
     Site            order(271,278..283,287)
                     /site_type="active"
                     /note="E3 interaction surface [active]"
                     /db_xref="CDD:133458"
     Site            281
                     /site_type="other"
                     /note="lipoyl attachment site [posttranslational
                     modification]"
                     /db_xref="CDD:133458"
     Region          345..929
                     /region_name="PRK11856"
                     /note="branched-chain alpha-keto acid dehydrogenase
                     subunit E2; Reviewed"
                     /db_xref="CDD:237001"
     CDS             1..929
                     /gene="PDHE2"
                     /locus_tag="TGME49_206610"
                     /old_locus_tag="TGME49_006610"
                     /coded_by="XM_018779368.1:1..2790"
                     /inference="protein motif:PFAM:PF00198"
                     /note="encoded by transcript TGME49_206610;
                     Signal peptide predicted by SignalP 2.0 HMM (probability
                     0.572) with cleavage site probability 0.212 at residue 24"
                     /db_xref="GeneID:7894015"
ORIGIN      
        1 malpspgilv hvslflaavs safglqlvps sspapsasst sfsgssapaf vapavssgsg
       61 vhstrprffa spsassassv sstnagqrpl sagsslqtre rrcgsrltss llcaaegtvr
      121 rqetavgssl rgavqeismp alsstmkegk vvtwskqvgd rvepgdvlmv vesdkadmdv
      181 eafdsgfmam hlvregdaap vgttvallae keedisliqa kglslisass spaadstpav
      241 tdllmpslsp slktarmtvw rkkegekvnk gdvlfvvesd kadmdveaph dgvlahiavr
      301 egvtvdvgst vgylapsaev asafknalsd saapaaanps tmpegaqeif mpalsstmts
      361 gkvskwnkav gdavhvgdtl mvvesdkadm dvesfdegyl aaitvaeges apvgqtvaii
      421 vpskddiakv qdaltaasta sssspahapl ssasspstps srlsssdsvs vsssqsgrpt
      481 tggdsrtaaf mkhgqalarw tspsvdqdvk dqlpegltgn dlqqewlqri eatmpttfaa
      541 lqanpqmqka llerlnlrvp pphtlrvspa pppylkravs tygaatagvp dtrasgidrt
      601 srgpardpsg qplatfnaie lakknklnle evkgtgtnrr itaadvrqhl hlpsdeatvv
      661 tskrenegki eslgvpppgs vpldamqkav arnmeatmdv pvfrvsrgiy vdkleamvqe
      721 lkqivaeqna aaiaaegpda pqqstvtmsv llakavaltl ekhpimnaay npkdggqiqh
      781 pgavnvamav svdgglltpv lrnvntksvf elsadwavlv dkarkrrlta eensagtfyi
      841 snlgmfgvsq fdavlpkgvg timavggtes vpffpktgtl dapagnpsvr rrmtvtitad
      901 hrhiygshaa aflkdfasll etrpsalli
//