KEGG   REACTION: R08700Help
Entry
R08700                      Reaction                               

Name
[enzyme]-S-sulfanylcysteine:tRNA-uridine sulfurtransferase (ATP-hydrolysing)
Definition
tRNA uridine + [Enzyme]-S-sulfanylcysteine + ATP <=> tRNA containing 2-thiouridine + [Enzyme]-cysteine + AMP + Diphosphate
Equation
Comment
multi-step reaction
incomplete reaction (donor is unidentified in vivo, dithiothreitol[CPD:C00265] in vitro)
Reaction class
RC02313  C15811_C15812
RC02315  C00868_C17322
Enzyme
Orthology
K00566  tRNA-uridine 2-sulfurtransferase [EC:2.8.1.13]
Reference
1  [PMID:12549933]
  Authors
Kambampati R, Lauhon CT.
  Title
MnmA and IscS are required for in vitro 2-thiouridine biosynthesis in Escherichia coli.
  Journal
Biochemistry 42:1109-17 (2003)
DOI:10.1021/bi026536+

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