KEGG   REACTION: R08700
Entry
R08700                      Reaction                               
Name
[enzyme]-S-sulfanylcysteine:tRNA-uridine sulfurtransferase (ATP-hydrolysing)
Definition
tRNA uridine + [Enzyme]-S-sulfanylcysteine + ATP <=> tRNA containing 2-thiouridine + [Enzyme]-cysteine + AMP + Diphosphate
Equation
C00868 + C15812 + C00002 <=> C17322 + C15811 + C00020 + C00013
Comment
multi-step reaction
incomplete reaction (donor is unidentified in vivo, dithiothreitol[CPD:C00265] in vitro)
Reaction class
RC02313  C15811_C15812
RC02315  C00868_C17322
Enzyme
2.8.1.13
Brite
Enzymatic reactions [BR:br08201]
 2. Transferase reactions
  2.8  Transferring sulfur-containing groups
   2.8.1  Sulfurtransferases
    2.8.1.13
     R08700  tRNA uridine + [Enzyme]-S-sulfanylcysteine + ATP <=> tRNA containing 2-thiouridine + [Enzyme]-cysteine + AMP + Diphosphate
Orthology
K00566  tRNA-uridine 2-sulfurtransferase [EC:2.8.1.13]
Reference
1  [PMID:12549933]
  Authors
Kambampati R, Lauhon CT.
  Title
MnmA and IscS are required for in vitro 2-thiouridine biosynthesis in Escherichia coli.
  Journal
Biochemistry 42:1109-17 (2003)
DOI:10.1021/bi026536+

  All links  
Ontology (1)   
   KEGG BRITE (1)   
Chemical substance (7)   
   KEGG COMPOUND (7)   
Chemical reaction (3)   
   KEGG ENZYME (1)   
   KEGG RCLASS (2)   
Gene (8759)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (8758)   
All databases (8770)   

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