KEGG   PATHWAY: sce03030
Entry
sce03030                    Pathway                                
Name
DNA replication - Saccharomyces cerevisiae (budding yeast)
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
Genetic Information Processing; Replication and repair
Pathway map
sce03030  DNA replication
sce03030

Organism
Saccharomyces cerevisiae (budding yeast) [GN:sce]
Gene
YAR007C  RFA1; replication factor A subunit protein RFA1 [KO:K07466]
YBL023C  MCM2; MCM DNA helicase complex subunit MCM2 [KO:K02540] [EC:5.6.2.3]
YBL035C  POL12; DNA-directed DNA polymerase alpha subunit POL12 [KO:K02321]
YBR087W  RFC5; replication factor C subunit 5 [KO:K10756]
YBR088C  POL30; proliferating cell nuclear antigen [KO:K04802]
YBR202W  MCM7; mini-chromosome maintenance complex protein 7 [KO:K02210] [EC:5.6.2.3]
YBR278W  DPB3; DNA polymerase epsilon noncatalytic subunit [KO:K03506] [EC:2.7.7.7]
YDL102W  POL3; DNA-directed DNA polymerase delta POL3 [KO:K02327] [EC:2.7.7.7]
YDL164C  CDC9; DNA ligase (ATP) CDC9 [KO:K10747] [EC:6.5.1.1 6.5.1.6 6.5.1.7]
YDR121W  DPB4; DNA polymerase epsilon noncatalytic subunit [KO:K02326] [EC:2.7.7.7]
YEL032W  MCM3; MCM DNA helicase complex subunit MCM3 [KO:K02541] [EC:5.6.2.3]
YGL201C  MCM6; MCM DNA helicase complex subunit MCM6 [KO:K02542] [EC:5.6.2.3]
YHR164C  DNA2; bifunctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2 [KO:K10742] [EC:5.6.2.3 3.1.-.-]
YIR008C  PRI1; DNA primase subunit PRI1 [KO:K02684] [EC:2.7.7.102]
YJL065C  DLS1; Dls1p [KO:K03506] [EC:2.7.7.7]
YJR006W  POL31; DNA-directed DNA polymerase delta subunit POL31 [KO:K02328]
YJR043C  POL32; DNA polymerase delta subunit POL32 [KO:K03504]
YJR068W  RFC2; replication factor C subunit 2 [KO:K10755]
YKL045W  PRI2; DNA primase subunit PRI2 [KO:K02685]
YKL113C  RAD27; multifunctional nuclease RAD27 [KO:K04799] [EC:3.1.-.-]
YLR274W  MCM5; MCM DNA helicase complex subunit MCM5 [KO:K02209] [EC:5.6.2.3]
YMR234W  RNH1; RNA-DNA hybrid ribonuclease [KO:K03469] [EC:3.1.26.4]
YNL072W  RNH201; ribonuclease H2 catalytic subunit RNH201 [KO:K10743] [EC:3.1.26.4]
YNL102W  POL1; DNA-directed DNA polymerase alpha catalytic subunit POL1 [KO:K02320] [EC:2.7.7.7]
YNL262W  POL2; DNA polymerase epsilon catalytic subunit [KO:K02324] [EC:2.7.7.7]
YNL290W  RFC3; replication factor C subunit 3 [KO:K10756]
YNL312W  RFA2; Rfa2p [KO:K10739]
YOL094C  RFC4; replication factor C subunit 4 [KO:K10755]
YOR217W  RFC1; replication factor C subunit 1 [KO:K10754]
YPR019W  MCM4; MCM DNA helicase complex subunit MCM4 [KO:K02212] [EC:5.6.2.3]
YPR175W  DPB2; DNA polymerase epsilon noncatalytic subunit [KO:K02325] [EC:2.7.7.7]
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
DOI:10.1016/S0092-8674(94)90362-X
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
DOI:10.1146/annurev.biochem.67.1.721
KO pathway
ko03030   

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