KEGG   PATHWAY: sce03030Help
Entry
sce03030                    Pathway                                

Name
DNA replication - Saccharomyces cerevisiae (budding yeast)
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
09120 Genetic Information Processing; 09124 Replication and repair
BRITE hierarchy
Pathway map
sce03030  DNA replication
sce03030

Ortholog table
Module
sce_M00261  DNA polymerase alpha / primase complex [PATH:sce03030]
sce_M00263  DNA polymerase epsilon complex [PATH:sce03030]
sce_M00285  MCM complex [PATH:sce03030]
sce_M00289  RF-C complex [PATH:sce03030]
Organism
Saccharomyces cerevisiae (budding yeast) [GN:sce]
Gene
YMR234W  RNH1; RNA-DNA hybrid ribonuclease [KO:K03469] [EC:3.1.26.4]
YAR007C  RFA1; replication factor A subunit protein RFA1 [KO:K07466]
YBR088C  POL30; proliferating cell nuclear antigen [KO:K04802]
YHR164C  DNA2; bifunctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2 [KO:K10742] [EC:3.6.4.12]
YKL113C  RAD27; multifunctional nuclease RAD27 [KO:K04799] [EC:3.-.-.-]
YDL164C  CDC9; DNA ligase (ATP) CDC9 [KO:K10747] [EC:6.5.1.7 6.5.1.6 6.5.1.1]
YNL102W  POL1; DNA-directed DNA polymerase alpha catalytic subunit POL1 [KO:K02320] [EC:2.7.7.7]
YBL035C  POL12; DNA-directed DNA polymerase alpha subunit POL12 [KO:K02321]
YIR008C  PRI1; DNA primase subunit PRI1 [KO:K02684] [EC:2.7.7.-]
YKL045W  PRI2; DNA primase subunit PRI2 [KO:K02685] [EC:2.7.7.-]
YDL102W  POL3; DNA-directed DNA polymerase delta POL3 [KO:K02327] [EC:2.7.7.7]
YJR006W  POL31; DNA-directed DNA polymerase delta subunit POL31 [KO:K02328]
YJR043C  POL32; DNA polymerase delta subunit POL32 [KO:K03504]
YNL262W  POL2; DNA polymerase epsilon catalytic subunit [KO:K02324] [EC:2.7.7.7]
YPR175W  DPB2; DNA polymerase epsilon noncatalytic subunit [KO:K02325] [EC:2.7.7.7]
YDR121W  DPB4; DNA polymerase epsilon noncatalytic subunit [KO:K02326] [EC:2.7.7.7]
YBR278W  DPB3; DNA polymerase epsilon noncatalytic subunit [KO:K03506] [EC:2.7.7.7]
YJL065C  DLS1; Dls1p [KO:K03506] [EC:2.7.7.7]
YBL023C  MCM2; MCM DNA helicase complex subunit MCM2 [KO:K02540] [EC:3.6.4.12]
YEL032W  MCM3; MCM DNA helicase complex subunit MCM3 [KO:K02541] [EC:3.6.4.12]
YPR019W  MCM4; MCM DNA helicase complex subunit MCM4 [KO:K02212] [EC:3.6.4.12]
YLR274W  MCM5; MCM DNA helicase complex subunit MCM5 [KO:K02209] [EC:3.6.4.12]
YGL201C  MCM6; MCM DNA helicase complex subunit MCM6 [KO:K02542] [EC:3.6.4.12]
YBR202W  MCM7; mini-chromosome maintenance complex protein 7 [KO:K02210] [EC:3.6.4.12]
YNL312W  RFA2; Rfa2p [KO:K10739]
YOR217W  RFC1; replication factor C subunit 1 [KO:K10754]
YJR068W  RFC2; replication factor C subunit 2 [KO:K10755]
YOL094C  RFC4; replication factor C subunit 4 [KO:K10755]
YNL290W  RFC3; replication factor C subunit 3 [KO:K10756]
YBR087W  RFC5; replication factor C subunit 5 [KO:K10756]
YNL072W  RNH201; ribonuclease H2 catalytic subunit RNH201 [KO:K10743] [EC:3.1.26.4]
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
DOI:10.1016/S0092-8674(94)90362-X
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
DOI:10.1146/annurev.biochem.67.1.721
KO pathway
ko03030   

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