KEGG   PATHWAY: sma03030Help
Entry
sma03030                    Pathway                                

Name
DNA replication - Streptomyces avermitilis
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
Genetic Information Processing; Replication and repair
BRITE hierarchy
Pathway map
sma03030  DNA replication
sma03030

Ortholog table
Organism
Streptomyces avermitilis [GN:sma]
Gene
SAVERM_6439  dnaQ1; putative DNA polymerase III epsilon subunit [KO:K02342] [EC:2.7.7.7]
SAVERM_2147  dnaQ3; putative DNA polymerase III epsilon subunit [KO:K02342] [EC:2.7.7.7]
SAVERM_6143  dnaE1; putative DNA polymerase III alpha subunit [KO:K02337] [EC:2.7.7.7]
SAVERM_4150  dnaZX; putative DNA polymerase III gamma and tau subunit [KO:K02343] [EC:2.7.7.7]
SAVERM_4623  holB; putative DNA polymerase III delta prime subunit [KO:K02341] [EC:2.7.7.7]
SAVERM_5558  holA; putative DNA polymerase III delta subunit [KO:K02340] [EC:2.7.7.7]
SAVERM_2004  putative siderophore biosynthesis protein/iron transport protein [KO:K02339] [EC:2.7.7.7]
SAVERM_4317  dnaN1; putative DNA polymerase III beta subunit [KO:K02338] [EC:2.7.7.7]
SAVERM_3362  dnaN2; putative DNA polymerase III beta subunit [KO:K02338] [EC:2.7.7.7]
SAVERM_4284  dnaB; putative replicative DNA helicase [KO:K02314] [EC:3.6.4.12]
SAVERM_5676  dnaG; putative DNA primase [KO:K02316] [EC:2.7.7.101]
SAVERM_4288  ssb1; putative single-stranded DNA-binding protein [KO:K03111]
SAVERM_5365  ssb2; putative single-strand DNA-binding protein [KO:K03111]
SAVERM_1138  rnhA; putative ribonuclease H [KO:K03469] [EC:3.1.26.4]
SAVERM_5877  putative bifunctional protein (ribonuclease H/phosphoglycerate mutase) [KO:K22316] [EC:3.1.26.4 3.1.3.73]
SAVERM_2453  rnhB; putative ribonuclease HII [KO:K03470] [EC:3.1.26.4]
SAVERM_6227  polA1; putative DNA polymerase I [KO:K02335] [EC:2.7.7.7]
SAVERM_4636  polA2; putative DNA polymerase I [KO:K02335] [EC:2.7.7.7]
SAVERM_2748  ligA; putative DNA ligase [KO:K01972] [EC:6.5.1.2]
SAVERM_7136  ligB; putative DNA ligase [KO:K10747] [EC:6.5.1.1 6.5.1.6 6.5.1.7]
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
DOI:10.1016/S0092-8674(94)90362-X
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
DOI:10.1146/annurev.biochem.67.1.721
KO pathway
ko03030   

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