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Database: UniProt/SWISS-PROT
Entry: CBIT_SALTY
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Original site: CBIT_SALTY 
ID   CBIT_SALTY              Reviewed;         192 AA.
AC   Q05632;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   18-SEP-2019, entry version 107.
DE   RecName: Full=Cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating);
DE            EC=2.1.1.196;
GN   Name=cbiT; OrderedLocusNames=STM2030;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF N-TERMINUS.
RC   STRAIN=LT2;
RX   PubMed=8501034; DOI=10.1128/jb.175.11.3303-3316.1993;
RA   Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S.,
RA   Church G.M.;
RT   "Characterization of the cobalamin (vitamin B12) biosynthetic genes of
RT   Salmonella typhimurium.";
RL   J. Bacteriol. 175:3303-3316(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M.,
RA   Waterston R., Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium
RT   LT2.";
RL   Nature 413:852-856(2001).
RN   [3]
RP   PROTEIN SEQUENCE OF 1-10.
RX   PubMed=1451790; DOI=10.1016/0014-5793(92)80213-z;
RA   Roessner C.A., Warren M.J., Santander P.J., Atshaves B.P., Ozaki S.,
RA   Stolowich N.J., Iida K., Scott A.I.;
RT   "Expression of 9 Salmonella typhimurium enzymes for cobinamide
RT   synthesis. Identification of the 11-methyl and 20-methyl transferases
RT   of corrin biosynthesis.";
RL   FEBS Lett. 301:73-78(1992).
RN   [4]
RP   FUNCTION.
RX   PubMed=16198574; DOI=10.1016/j.bmc.2005.08.062;
RA   Santander P.J., Kajiwara Y., Williams H.J., Scott A.I.;
RT   "Structural characterization of novel cobalt corrinoids synthesized by
RT   enzymes of the vitamin B12 anaerobic pathway.";
RL   Bioorg. Med. Chem. 14:724-731(2006).
CC   -!- FUNCTION: Catalyzes the methylation of C-15 in cobalt-precorrin-6B
CC       followed by the decarboxylation of C-12 to form cobalt-precorrin-
CC       7. {ECO:0000269|PubMed:16198574}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Co-precorrin-6B + S-adenosyl-L-methionine = Co-precorrin-
CC         7 + CO2 + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:36067,
CC         ChEBI:CHEBI:16526, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:70791, ChEBI:CHEBI:72780; EC=2.1.1.196;
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic
CC       route): step 8/10.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       Bacterial-type CbiT family. {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to be a precorrin-8w
CC       decarboxylase. {ECO:0000305}.
DR   EMBL; L12006; AAA27257.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL20934.1; -; Genomic_DNA.
DR   RefSeq; NP_460975.1; NC_003197.2.
DR   RefSeq; WP_000650985.1; NC_003197.2.
DR   SMR; Q05632; -.
DR   PaxDb; Q05632; -.
DR   PRIDE; Q05632; -.
DR   EnsemblBacteria; AAL20934; AAL20934; STM2030.
DR   GeneID; 1253551; -.
DR   KEGG; stm:STM2030; -.
DR   PATRIC; fig|99287.12.peg.2152; -.
DR   eggNOG; ENOG4107XSI; Bacteria.
DR   eggNOG; COG2242; LUCA.
DR   HOGENOM; HOG000290407; -.
DR   KO; K02191; -.
DR   OMA; RCKFVYA; -.
DR   PhylomeDB; Q05632; -.
DR   BioCyc; MetaCyc:MONOMER-13226; -.
DR   BioCyc; SENT99287:STM2030-MONOMER; -.
DR   UniPathway; UPA00148; UER00229.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0008276; F:protein methyltransferase activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR014008; Cbl_synth_MTase_CbiT.
DR   InterPro; IPR029063; SAM-dependent_MTases.
DR   InterPro; IPR007848; Small_mtfrase_dom.
DR   Pfam; PF05175; MTS; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   TIGRFAMs; TIGR02469; CbiT; 1.
PE   1: Evidence at protein level;
KW   Cobalamin biosynthesis; Complete proteome; Direct protein sequencing;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN         1    192       Cobalt-precorrin-6B C(15)-
FT                                methyltransferase (decarboxylating).
FT                                /FTId=PRO_0000134949.
FT   REGION       39     43       S-adenosyl-L-methionine binding.
FT                                {ECO:0000250}.
FT   BINDING      15     15       S-adenosyl-L-methionine. {ECO:0000250}.
FT   BINDING      62     62       S-adenosyl-L-methionine. {ECO:0000250}.
FT   BINDING      90     90       S-adenosyl-L-methionine; via amide
FT                                nitrogen. {ECO:0000250}.
SQ   SEQUENCE   192 AA;  20746 MW;  70D3CE0D2DDF024B CRC64;
     MKDELFLRGE NVPMTKEAVR ALALSKLELH RASHLIDVGA GTGSVSIEAA LQFPSLQVTA
     IERNPAALRL LDENRQRFAC GNIDILPGEA PMTITGKADA VFMGGSGGHL TALIDWAMGH
     LHPGGRLVMT FILQENLHSA LAHLAHIGAC RMDCVQLQLS SLTPLGAGHY FKPNNPVFVI
     ACQKEENHVR DI
//
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