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Database: UniProt/SWISS-PROT
Entry: DNLI_ARCFU
LinkDB: DNLI_ARCFU
Original site: DNLI_ARCFU 
ID   DNLI_ARCFU              Reviewed;         555 AA.
AC   O29632;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   20-JUN-2001, sequence version 2.
DT   10-OCT-2018, entry version 117.
DE   RecName: Full=DNA ligase {ECO:0000255|HAMAP-Rule:MF_00407};
DE            EC=6.5.1.1 {ECO:0000255|HAMAP-Rule:MF_00407};
DE   AltName: Full=Polydeoxyribonucleotide synthase [ATP] {ECO:0000255|HAMAP-Rule:MF_00407};
GN   Name=lig {ECO:0000255|HAMAP-Rule:MF_00407}; OrderedLocusNames=AF_0623;
OS   Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM
OS   9628 / NBRC 100126).
OC   Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales;
OC   Archaeoglobaceae; Archaeoglobus.
OX   NCBI_TaxID=224325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126;
RX   PubMed=9389475; DOI=10.1038/37052;
RA   Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E.,
RA   Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D.,
RA   Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C.,
RA   Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G., Gill S.R.,
RA   Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J.,
RA   Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A.,
RA   Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A.,
RA   Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P.,
RA   Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C.,
RA   Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O.,
RA   Woese C.R., Venter J.C.;
RT   "The complete genome sequence of the hyperthermophilic, sulphate-
RT   reducing archaeon Archaeoglobus fulgidus.";
RL   Nature 390:364-370(1997).
CC   -!- FUNCTION: DNA ligase that seals nicks in double-stranded DNA
CC       during DNA replication, DNA recombination and DNA repair.
CC       {ECO:0000255|HAMAP-Rule:MF_00407}.
CC   -!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n)-3'-hydroxyl +
CC       5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) +
CC       AMP + diphosphate. {ECO:0000255|HAMAP-Rule:MF_00407}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00407};
CC   -!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00407}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB90616.1; Type=Erroneous initiation; Evidence={ECO:0000305};
DR   EMBL; AE000782; AAB90616.1; ALT_INIT; Genomic_DNA.
DR   PIR; G69327; G69327.
DR   RefSeq; WP_048064635.1; NC_000917.1.
DR   PDB; 3GDE; X-ray; 2.30 A; A=1-555.
DR   PDBsum; 3GDE; -.
DR   ProteinModelPortal; O29632; -.
DR   SMR; O29632; -.
DR   STRING; 224325.AF0623; -.
DR   PRIDE; O29632; -.
DR   EnsemblBacteria; AAB90616; AAB90616; AF_0623.
DR   GeneID; 24794226; -.
DR   KEGG; afu:AF_0623; -.
DR   eggNOG; arCOG01347; Archaea.
DR   eggNOG; COG1793; LUCA.
DR   KO; K10747; -.
DR   OMA; WLFEESY; -.
DR   OrthoDB; POG093Z03L0; -.
DR   BioCyc; AFUL224325:G1G17-648-MONOMER; -.
DR   BRENDA; 6.5.1.1; 414.
DR   EvolutionaryTrace; O29632; -.
DR   Proteomes; UP000002199; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0071897; P:DNA biosynthetic process; IEA:InterPro.
DR   GO; GO:0051103; P:DNA ligation involved in DNA repair; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.3260.10; -; 1.
DR   HAMAP; MF_00407; DNA_ligase; 1.
DR   InterPro; IPR022865; DNA_ligae_ATP-dep_bac/arc.
DR   InterPro; IPR000977; DNA_ligase_ATP-dep.
DR   InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR   InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR   InterPro; IPR016059; DNA_ligase_ATP-dep_CS.
DR   InterPro; IPR012308; DNA_ligase_ATP-dep_N.
DR   InterPro; IPR036599; DNA_ligase_N_sf.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Pfam; PF04679; DNA_ligase_A_C; 1.
DR   Pfam; PF01068; DNA_ligase_A_M; 1.
DR   Pfam; PF04675; DNA_ligase_A_N; 1.
DR   SUPFAM; SSF117018; SSF117018; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00574; dnl1; 1.
DR   PROSITE; PS00697; DNA_LIGASE_A1; 1.
DR   PROSITE; PS00333; DNA_LIGASE_A2; 1.
DR   PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Cell cycle; Cell division;
KW   Complete proteome; DNA damage; DNA recombination; DNA repair;
KW   DNA replication; Ligase; Magnesium; Metal-binding; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN         1    555       DNA ligase.
FT                                /FTId=PRO_0000059601.
FT   ACT_SITE    249    249       N6-AMP-lysine intermediate.
FT                                {ECO:0000255|HAMAP-Rule:MF_00407}.
FT   BINDING     247    247       ATP. {ECO:0000255|HAMAP-Rule:MF_00407}.
FT   BINDING     254    254       ATP. {ECO:0000255|HAMAP-Rule:MF_00407}.
FT   BINDING     269    269       ATP. {ECO:0000255|HAMAP-Rule:MF_00407}.
FT   BINDING     298    298       ATP. {ECO:0000255|HAMAP-Rule:MF_00407}.
FT   BINDING     337    337       ATP. {ECO:0000255|HAMAP-Rule:MF_00407}.
FT   BINDING     411    411       ATP. {ECO:0000255|HAMAP-Rule:MF_00407}.
FT   BINDING     417    417       ATP. {ECO:0000255|HAMAP-Rule:MF_00407}.
FT   HELIX         3     13       {ECO:0000244|PDB:3GDE}.
FT   HELIX        19     31       {ECO:0000244|PDB:3GDE}.
FT   HELIX        36     46       {ECO:0000244|PDB:3GDE}.
FT   HELIX        63     74       {ECO:0000244|PDB:3GDE}.
FT   HELIX        78     88       {ECO:0000244|PDB:3GDE}.
FT   HELIX        91    105       {ECO:0000244|PDB:3GDE}.
FT   HELIX       114    126       {ECO:0000244|PDB:3GDE}.
FT   HELIX       132    146       {ECO:0000244|PDB:3GDE}.
FT   HELIX       149    159       {ECO:0000244|PDB:3GDE}.
FT   HELIX       169    179       {ECO:0000244|PDB:3GDE}.
FT   HELIX       184    194       {ECO:0000244|PDB:3GDE}.
FT   HELIX       197    213       {ECO:0000244|PDB:3GDE}.
FT   STRAND      227    230       {ECO:0000244|PDB:3GDE}.
FT   HELIX       234    240       {ECO:0000244|PDB:3GDE}.
FT   STRAND      241    249       {ECO:0000244|PDB:3GDE}.
FT   STRAND      251    259       {ECO:0000244|PDB:3GDE}.
FT   STRAND      265    268       {ECO:0000244|PDB:3GDE}.
FT   HELIX       275    277       {ECO:0000244|PDB:3GDE}.
FT   HELIX       279    288       {ECO:0000244|PDB:3GDE}.
FT   STRAND      291    303       {ECO:0000244|PDB:3GDE}.
FT   STRAND      306    308       {ECO:0000244|PDB:3GDE}.
FT   HELIX       310    318       {ECO:0000244|PDB:3GDE}.
FT   HELIX       324    329       {ECO:0000244|PDB:3GDE}.
FT   STRAND      332    345       {ECO:0000244|PDB:3GDE}.
FT   HELIX       351    361       {ECO:0000244|PDB:3GDE}.
FT   STRAND      366    370       {ECO:0000244|PDB:3GDE}.
FT   STRAND      373    377       {ECO:0000244|PDB:3GDE}.
FT   HELIX       379    391       {ECO:0000244|PDB:3GDE}.
FT   STRAND      396    400       {ECO:0000244|PDB:3GDE}.
FT   STRAND      411    421       {ECO:0000244|PDB:3GDE}.
FT   STRAND      425    434       {ECO:0000244|PDB:3GDE}.
FT   HELIX       439    441       {ECO:0000244|PDB:3GDE}.
FT   STRAND      442    451       {ECO:0000244|PDB:3GDE}.
FT   TURN        453    455       {ECO:0000244|PDB:3GDE}.
FT   STRAND      458    464       {ECO:0000244|PDB:3GDE}.
FT   HELIX       470    480       {ECO:0000244|PDB:3GDE}.
FT   HELIX       481    483       {ECO:0000244|PDB:3GDE}.
FT   STRAND      484    488       {ECO:0000244|PDB:3GDE}.
FT   STRAND      491    494       {ECO:0000244|PDB:3GDE}.
FT   STRAND      499    503       {ECO:0000244|PDB:3GDE}.
FT   STRAND      505    509       {ECO:0000244|PDB:3GDE}.
FT   STRAND      511    513       {ECO:0000244|PDB:3GDE}.
FT   STRAND      516    521       {ECO:0000244|PDB:3GDE}.
FT   STRAND      523    527       {ECO:0000244|PDB:3GDE}.
FT   HELIX       533    535       {ECO:0000244|PDB:3GDE}.
FT   HELIX       539    547       {ECO:0000244|PDB:3GDE}.
SQ   SEQUENCE   555 AA;  63553 MW;  D050FE80B2341EDB CRC64;
     MLFAEFAEFC ERLEKISSTL ELTARIAAFL QKIEDERDLY DVVLFITGKV YPPWDERELG
     VGIGLLYEAL ENVSGVKRSE IESMIREYGD LGLVAEQLIK KKKMTTLAFE ELTVRKVRET
     FDEIASLTGE GSMKRKIMLL TGLYGLATPL EARYLTRLIL NEMRLGVGEG IMRDAIARAF
     RADPETVERA YMITNDLGRV AVVAKKEGEE GLRKMKIEIH IPVRMMLAQV AESLESAVRE
     MRTAAVEWKF DGSRVQVHWD GSRVTIYSRR LENVTNALPD IVEEIKKSVK PGVILDGEVI
     AVKEGKPMPF QHVLRRFRRK HDVAKMVEKI PLEAHFFDIL YHDGECIDLP LRERRKLLES
     AVNESEKIKL AKQIVTDSVD EVRKMYDEAI SAGHEGVMIK LPSSPYIPGK RGKNWLKVKA
     IMETLDLVVV GGEWGEGKRS HWLSSFELAC LDPVTGKLLK VGRVATGFTE EDLEELTEMF
     RPLIVSQQGK KVEFIPKYVF EVAYQEIQKS PKYESGYALR FPRFVRLRDD KDVDEADTIE
     RVENLYKLQF EVKRQ
//
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