ID EFTU_NITHX Reviewed; 396 AA.
AC Q1QN32;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 1.
DT 22-NOV-2017, entry version 81.
DE RecName: Full=Elongation factor Tu {ECO:0000255|HAMAP-Rule:MF_00118};
DE Short=EF-Tu {ECO:0000255|HAMAP-Rule:MF_00118};
GN Name=tuf {ECO:0000255|HAMAP-Rule:MF_00118};
GN OrderedLocusNames=Nham_1543;
OS Nitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC Bradyrhizobiaceae; Nitrobacter.
OX NCBI_TaxID=323097;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 10229 / NCIMB 13809 / X14;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Ward B.,
RA Arp D., Klotz M., Stein L., O'Mullan G., Starkenburg S., Sayavedra L.,
RA Poret-Peterson A.T., Gentry M.E., Bruce D., Richardson P.;
RT "Complete sequence of chromosome of Nitrobacter hamburgensis X14.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: This protein promotes the GTP-dependent binding of
CC aminoacyl-tRNA to the A-site of ribosomes during protein
CC biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00118}.
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00118}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00118}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00118}.
DR EMBL; CP000319; ABE62365.1; -; Genomic_DNA.
DR RefSeq; WP_011510053.1; NC_007964.1.
DR ProteinModelPortal; Q1QN32; -.
DR SMR; Q1QN32; -.
DR STRING; 323097.Nham_1543; -.
DR PRIDE; Q1QN32; -.
DR EnsemblBacteria; ABE62365; ABE62365; Nham_1543.
DR KEGG; nha:Nham_1543; -.
DR eggNOG; ENOG4105CGV; Bacteria.
DR eggNOG; COG0050; LUCA.
DR HOGENOM; HOG000229290; -.
DR KO; K02358; -.
DR OMA; YGHIDCP; -.
DR OrthoDB; POG091H00LA; -.
DR Proteomes; UP000001953; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR CDD; cd03697; EFTU_II; 1.
DR HAMAP; MF_00118_B; EF_Tu_B; 1.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR033720; EFTU_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; TF_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004541; Transl_elong_EFTu/EF1A_bac/org.
DR InterPro; IPR004160; Transl_elong_EFTu/EF1A_C.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF03143; GTP_EFTU_D3; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF50465; SSF50465; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00485; EF-Tu; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Elongation factor; GTP-binding;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1 396 Elongation factor Tu.
FT /FTId=PRO_1000015711.
FT DOMAIN 10 206 tr-type G.
FT NP_BIND 19 26 GTP. {ECO:0000255|HAMAP-Rule:MF_00118}.
FT NP_BIND 81 85 GTP. {ECO:0000255|HAMAP-Rule:MF_00118}.
FT NP_BIND 136 139 GTP. {ECO:0000255|HAMAP-Rule:MF_00118}.
FT REGION 19 26 G1. {ECO:0000250}.
FT REGION 60 64 G2. {ECO:0000250}.
FT REGION 81 84 G3. {ECO:0000250}.
FT REGION 136 139 G4. {ECO:0000250}.
FT REGION 174 176 G5. {ECO:0000250}.
SQ SEQUENCE 396 AA; 43182 MW; 136130FF5F43DFE2 CRC64;
MAKAKFERNK PHCNIGTIGH VDHGKTSLTA AITKVLAEAG GATFTAYDQI DKAPEEKARG
ITISTSHVEY ETPNRHYAHV DCPGHADYVK NMITGAAQMD GAILVVSAAD GPMPQTREHI
LLARQVGVPA IVVFLNKCDM VDDPELLELV EMEVRELLSK YDFPGDDIPI IKGSALAVLE
NSDPKLGHDA VLELMKAVDA YIPQPERPVD QPFLMPVEDV FSISGRGTVV TGRVERGIVK
VGEEIEIVGI RPTQKTTVTG VEMFRKLLDQ GQAGDNIGAL LRGTKREDVE RGQVLCKPGS
VKPHTKFKAE AYILTKEEGG RHTPFFTNYR PQFYFRTTDV TGVVHLPAGT EMVMPGDNVA
MEVHLIVPIA MEEKLRFAIR EGGRTVGAGV VASIIE
//
