ID EFTU_RHIME Reviewed; 391 AA.
AC Q925Y6;
DT 19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 10-OCT-2018, entry version 101.
DE RecName: Full=Elongation factor Tu {ECO:0000255|HAMAP-Rule:MF_00118};
DE Short=EF-Tu {ECO:0000255|HAMAP-Rule:MF_00118};
GN Name=tufA {ECO:0000255|HAMAP-Rule:MF_00118}; OrderedLocusNames=R01339;
GN ORFNames=SMc01311;
GN and
GN Name=tufB {ECO:0000255|HAMAP-Rule:MF_00118}; OrderedLocusNames=R01354;
GN ORFNames=SMc01326;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D.,
RA Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U.,
RA Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont
RT Sinorhizobium meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F.,
RA Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M.,
RA Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D.,
RA Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V.,
RA Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B.,
RA Ramsperger U., Surzycki R., Thebault P., Vandenbol M.,
RA Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- FUNCTION: This protein promotes the GTP-dependent binding of
CC aminoacyl-tRNA to the A-site of ribosomes during protein
CC biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00118}.
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00118}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00118}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00118}.
DR EMBL; AL591688; CAC45918.1; -; Genomic_DNA.
DR EMBL; AL591688; CAC45933.1; -; Genomic_DNA.
DR RefSeq; NP_385445.1; NC_003047.1.
DR RefSeq; NP_385460.1; NC_003047.1.
DR RefSeq; WP_010969189.1; NC_003047.1.
DR ProteinModelPortal; Q925Y6; -.
DR SMR; Q925Y6; -.
DR STRING; 266834.SMc01311; -.
DR PRIDE; Q925Y6; -.
DR EnsemblBacteria; CAC45918; CAC45918; SMc01326.
DR EnsemblBacteria; CAC45933; CAC45933; SMc01311.
DR GeneID; 1232990; -.
DR GeneID; 1233006; -.
DR KEGG; sme:SMc01311; -.
DR KEGG; sme:SMc01326; -.
DR PATRIC; fig|266834.11.peg.2754; -.
DR eggNOG; ENOG4105CGV; Bacteria.
DR eggNOG; COG0050; LUCA.
DR HOGENOM; HOG000229290; -.
DR KO; K02358; -.
DR OMA; YGHIDCP; -.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-UniRule.
DR CDD; cd03697; EFTU_II; 1.
DR HAMAP; MF_00118_B; EF_Tu_B; 1.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR033720; EFTU_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; TF_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004541; Transl_elong_EFTu/EF1A_bac/org.
DR InterPro; IPR004160; Transl_elong_EFTu/EF1A_C.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF03143; GTP_EFTU_D3; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF50465; SSF50465; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00485; EF-Tu; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Elongation factor; GTP-binding;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1 391 Elongation factor Tu.
FT /FTId=PRO_0000091371.
FT DOMAIN 10 201 tr-type G.
FT NP_BIND 19 26 GTP. {ECO:0000255|HAMAP-Rule:MF_00118}.
FT NP_BIND 76 80 GTP. {ECO:0000255|HAMAP-Rule:MF_00118}.
FT NP_BIND 131 134 GTP. {ECO:0000255|HAMAP-Rule:MF_00118}.
FT REGION 19 26 G1. {ECO:0000250}.
FT REGION 55 59 G2. {ECO:0000250}.
FT REGION 76 79 G3. {ECO:0000250}.
FT REGION 131 134 G4. {ECO:0000250}.
FT REGION 169 171 G5. {ECO:0000250}.
SQ SEQUENCE 391 AA; 42749 MW; 8846A34F44753693 CRC64;
MAKSKFERNK PHVNIGTIGH VDHGKTSLTA AITKYFGEFK AYDQIDAAPE EKARGITIST
AHVEYETPNR HYAHVDCPGH ADYVKNMITG AAQMDGAILV VSAADGPMPQ TREHILLARQ
VGVPAIVVFL NKVDQVDDAE LLELVELEVR ELLSSYEFPG DDIPIVKGSA LAALEDSDKK
IGEDAIRELM AAVDAYIPTP ERPIDQPFLM PIEDVFSISG RGTVVTGRVE RGIVKVGEEI
EIVGIRPTTK TTCTGVEMFR KLLDQGQAGD NIGALLRGVD RNGVERGQIL CKPGSVKPHR
KFKAEAYILT KEEGGRHTPF FTNYRPQFYF RTTDVTGIVT LPEGTEMVMP GDNVTVDVEL
IVPIAMEEKL RFAIREGGRT VGAGIVASIV E
//
