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Database: UniProt/SWISS-PROT
Entry: HXK_KLULA
LinkDB: HXK_KLULA
Original site: HXK_KLULA 
ID   HXK_KLULA               Reviewed;         485 AA.
AC   P33284;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   23-MAY-2018, entry version 127.
DE   RecName: Full=Hexokinase;
DE            EC=2.7.1.1;
GN   Name=RAG5; OrderedLocusNames=KLLA0D11352g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC
OS   1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 76492 / CBS 2359/152 / CLIB 210, and PM6-7A;
RX   PubMed=8321195; DOI=10.1128/MCB.13.7.3882;
RA   Prior C., Mamessier P., Fukuhara H., Chen X.J., Wesolowski-Louvel M.;
RT   "The hexokinase gene is required for transcriptional regulation of the
RT   glucose transporter gene RAG1 in Kluyveromyces lactis.";
RL   Mol. Cell. Biol. 13:3882-3889(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 /
RC   WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S.,
RA   Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E.,
RA   Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V.,
RA   Barnay S., Blanchin S., Beckerich J.-M., Beyne E., Bleykasten C.,
RA   Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A.,
RA   Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A.,
RA   Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R.,
RA   Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H.,
RA   Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O.,
RA   Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A.,
RA   Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B.,
RA   Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A.,
RA   Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J.,
RA   Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
RN   [3]
RP   PROTEIN SEQUENCE OF 2-21; 136-163 AND 198-226, MASS SPECTROMETRY,
RP   SUBUNIT, AND PHOSPHORYLATION AT SER-15.
RC   STRAIN=ATCC 76492 / CBS 2359/152 / CLIB 210;
RX   PubMed=12882981; DOI=10.1074/jbc.M305706200;
RA   Baer D., Golbik R., Huebner G., Lilie H., Mueller E.-C., Naumann M.,
RA   Otto A., Reuter R., Breunig K.D., Kriegel T.M.;
RT   "The unique hexokinase of Kluyveromyces lactis. Molecular and
RT   functional characterization and evaluation of a role in glucose
RT   signaling.";
RL   J. Biol. Chem. 278:39280-39286(2003).
CC   -!- CATALYTIC ACTIVITY: ATP + D-hexose = ADP + D-hexose 6-phosphate.
CC   -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC   -!- SUBUNIT: Monomer and homodimer. The monomeric form is active, the
CC       homodimeric form inactive. {ECO:0000269|PubMed:12882981}.
CC   -!- MASS SPECTROMETRY: Mass=53476; Mass_error=5; Method=Electrospray;
CC       Range=2-485; Evidence={ECO:0000269|PubMed:12882981};
CC   -!- SIMILARITY: Belongs to the hexokinase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU01084, ECO:0000305}.
DR   EMBL; X61680; CAA43855.1; -; Genomic_DNA.
DR   EMBL; CR382124; CAH00663.1; -; Genomic_DNA.
DR   PIR; A48132; A48132.
DR   RefSeq; XP_453567.1; XM_453567.1.
DR   PDB; 3O08; X-ray; 2.00 A; A/B=1-485.
DR   PDB; 3O1B; X-ray; 2.80 A; A=1-485.
DR   PDB; 3O1W; X-ray; 1.66 A; A/B=1-485.
DR   PDB; 3O4W; X-ray; 1.61 A; A/B=1-485.
DR   PDB; 3O5B; X-ray; 1.97 A; A/B=1-485.
DR   PDB; 3O6W; X-ray; 1.48 A; A/B=1-485.
DR   PDB; 3O80; X-ray; 2.18 A; A=1-485.
DR   PDB; 3O8M; X-ray; 1.42 A; A=1-485.
DR   PDB; 4JAX; X-ray; 2.26 A; A/B/C/D/E/F=1-485.
DR   PDBsum; 3O08; -.
DR   PDBsum; 3O1B; -.
DR   PDBsum; 3O1W; -.
DR   PDBsum; 3O4W; -.
DR   PDBsum; 3O5B; -.
DR   PDBsum; 3O6W; -.
DR   PDBsum; 3O80; -.
DR   PDBsum; 3O8M; -.
DR   PDBsum; 4JAX; -.
DR   ProteinModelPortal; P33284; -.
DR   SMR; P33284; -.
DR   STRING; 284590.XP_453567.1; -.
DR   iPTMnet; P33284; -.
DR   PRIDE; P33284; -.
DR   GeneID; 2893280; -.
DR   KEGG; kla:KLLA0D11352g; -.
DR   eggNOG; KOG1369; Eukaryota.
DR   eggNOG; COG5026; LUCA.
DR   HOGENOM; HOG000162670; -.
DR   InParanoid; P33284; -.
DR   KO; K00844; -.
DR   OMA; ERQFFRA; -.
DR   OrthoDB; EOG092C2JW4; -.
DR   BRENDA; 2.7.1.1; 2825.
DR   SABIO-RK; P33284; -.
DR   UniPathway; UPA00242; -.
DR   EvolutionaryTrace; P33284; -.
DR   Proteomes; UP000000598; Chromosome D.
DR   GO; GO:0005623; C:cell; IEA:GOC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR   GO; GO:0004396; F:hexokinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001678; P:cellular glucose homeostasis; IEA:InterPro.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-KW.
DR   GO; GO:0019318; P:hexose metabolic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR001312; Hexokinase.
DR   InterPro; IPR019807; Hexokinase_BS.
DR   InterPro; IPR022673; Hexokinase_C.
DR   InterPro; IPR022672; Hexokinase_N.
DR   PANTHER; PTHR19443; PTHR19443; 1.
DR   Pfam; PF00349; Hexokinase_1; 1.
DR   Pfam; PF03727; Hexokinase_2; 1.
DR   PROSITE; PS00378; HEXOKINASE_1; 1.
DR   PROSITE; PS51748; HEXOKINASE_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Allosteric enzyme; ATP-binding; Complete proteome;
KW   Direct protein sequencing; Glycolysis; Kinase; Nucleotide-binding;
KW   Phosphoprotein; Reference proteome; Transferase.
FT   INIT_MET      1      1       Removed. {ECO:0000269|PubMed:12882981}.
FT   CHAIN         2    485       Hexokinase.
FT                                /FTId=PRO_0000197608.
FT   DOMAIN       21    468       Hexokinase. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01084}.
FT   REGION       75    208       Hexokinase small subdomain.
FT                                {ECO:0000255|PROSITE-ProRule:PRU01084}.
FT   REGION      151    177       Glucose-binding. {ECO:0000255}.
FT   REGION      209    457       Hexokinase large subdomain.
FT                                {ECO:0000255|PROSITE-ProRule:PRU01084}.
FT   BINDING     111    111       ATP. {ECO:0000250}.
FT   MOD_RES      15     15       Phosphoserine.
FT                                {ECO:0000269|PubMed:12882981}.
FT   CONFLICT    206    206       A -> R (in Ref. 1; CAA43855).
FT                                {ECO:0000305}.
FT   HELIX        21     34       {ECO:0000244|PDB:3O8M}.
FT   HELIX        38     56       {ECO:0000244|PDB:3O8M}.
FT   STRAND       57     59       {ECO:0000244|PDB:3O8M}.
FT   STRAND       61     63       {ECO:0000244|PDB:3O80}.
FT   STRAND       79    103       {ECO:0000244|PDB:3O8M}.
FT   STRAND      105    113       {ECO:0000244|PDB:3O8M}.
FT   HELIX       118    120       {ECO:0000244|PDB:3O8M}.
FT   HELIX       123    141       {ECO:0000244|PDB:3O8M}.
FT   STRAND      150    156       {ECO:0000244|PDB:3O8M}.
FT   STRAND      158    161       {ECO:0000244|PDB:3O6W}.
FT   STRAND      163    166       {ECO:0000244|PDB:3O6W}.
FT   HELIX       187    197       {ECO:0000244|PDB:3O8M}.
FT   STRAND      202    208       {ECO:0000244|PDB:3O8M}.
FT   HELIX       210    221       {ECO:0000244|PDB:3O8M}.
FT   STRAND      225    242       {ECO:0000244|PDB:3O8M}.
FT   HELIX       243    245       {ECO:0000244|PDB:3O8M}.
FT   HELIX       247    249       {ECO:0000244|PDB:3O8M}.
FT   TURN        250    252       {ECO:0000244|PDB:3O8M}.
FT   STRAND      262    266       {ECO:0000244|PDB:3O8M}.
FT   HELIX       269    271       {ECO:0000244|PDB:3O8M}.
FT   TURN        272    275       {ECO:0000244|PDB:3O8M}.
FT   STRAND      277    279       {ECO:0000244|PDB:3O8M}.
FT   HELIX       283    291       {ECO:0000244|PDB:3O8M}.
FT   STRAND      292    294       {ECO:0000244|PDB:3O8M}.
FT   HELIX       299    304       {ECO:0000244|PDB:3O8M}.
FT   TURN        306    308       {ECO:0000244|PDB:3O8M}.
FT   HELIX       309    322       {ECO:0000244|PDB:3O8M}.
FT   STRAND      325    327       {ECO:0000244|PDB:3O8M}.
FT   HELIX       333    336       {ECO:0000244|PDB:3O8M}.
FT   HELIX       344    351       {ECO:0000244|PDB:3O8M}.
FT   HELIX       358    368       {ECO:0000244|PDB:3O8M}.
FT   HELIX       374    406       {ECO:0000244|PDB:3O8M}.
FT   STRAND      409    417       {ECO:0000244|PDB:3O8M}.
FT   HELIX       418    422       {ECO:0000244|PDB:3O8M}.
FT   HELIX       426    438       {ECO:0000244|PDB:3O8M}.
FT   HELIX       445    447       {ECO:0000244|PDB:3O8M}.
FT   STRAND      449    454       {ECO:0000244|PDB:3O8M}.
FT   TURN        458    460       {ECO:0000244|PDB:3O8M}.
FT   HELIX       461    476       {ECO:0000244|PDB:3O8M}.
FT   STRAND      480    482       {ECO:0000244|PDB:3O8M}.
SQ   SEQUENCE   485 AA;  53610 MW;  3E93D80C92663FB0 CRC64;
     MVRLGPKKPP ARKGSMADVP ANLMEQIHGL ETLFTVSSEK MRSIVKHFIS ELDKGLSKKG
     GNIPMIPGWV VEYPTGKETG DFLALDLGGT NLRVVLVKLG GNHDFDTTQN KYRLPDHLRT
     GTSEQLWSFI AKCLKEFVDE WYPDGVSEPL PLGFTFSYPA SQKKINSGVL QRWTKGFDIE
     GVEGHDVVPM LQEQIEKLNI PINVVALIND TTGTLVASLY TDPQTKMGII IGTGVNGAYY
     DVVSGIEKLE GLLPEDIGPD SPMAINCEYG SFDNEHLVLP RTKYDVIIDE ESPRPGQQAF
     EKMTSGYYLG EIMRLVLLDL YDSGFIFKDQ DISKLKEAYV MDTSYPSKIE DDPFENLEDT
     DDLFKTNLNI ETTVVERKLI RKLAELVGTR AARLTVCGVS AICDKRGYKT AHIAADGSVF
     NRYPGYKEKA AQALKDIYNW DVEKMEDHPI QLVAAEDGSG VGAAIIACLT QKRLAAGKSV
     GIKGE
//
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