ID TNFB_RAT Reviewed; 202 AA.
AC Q06332;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 24-JAN-2024, entry version 143.
DE RecName: Full=Lymphotoxin-alpha;
DE Short=LT-alpha;
DE AltName: Full=TNF-beta;
DE AltName: Full=Tumor necrosis factor ligand superfamily member 1;
DE Flags: Precursor;
GN Name=Lta; Synonyms=Tnfb, Tnfsf1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Sprague-Dawley; TISSUE=Testis;
RX PubMed=8224868; DOI=10.1016/0378-1119(93)90200-m;
RA Kwon J., Chung I.Y., Benveniste E.N.;
RT "Cloning and sequence analysis of the rat tumor necrosis factor-encoding
RT genes.";
RL Gene 132:227-236(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15060004; DOI=10.1101/gr.1987704;
RA Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H.,
RA Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.;
RT "The genomic sequence and comparative analysis of the rat major
RT histocompatibility complex.";
RL Genome Res. 14:631-639(2004).
CC -!- FUNCTION: Cytokine that in its homotrimeric form binds to
CC TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (By similarity). In
CC its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is
CC produced by lymphocytes and is cytotoxic for a wide range of tumor
CC cells in vitro and in vivo. {ECO:0000250|UniProtKB:P01374}.
CC -!- SUBUNIT: Homotrimer, and heterotrimer of either two LTB and one LTA
CC subunits or (less prevalent) two LTA and one LTB subunits. Interacts
CC with TNFRSF14. {ECO:0000250|UniProtKB:P01374}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Membrane {ECO:0000250}.
CC Note=The homotrimer is secreted. The heterotrimer is membrane-
CC associated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the tumor necrosis factor family. {ECO:0000305}.
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DR EMBL; L00981; AAA16276.1; -; Genomic_DNA.
DR EMBL; BX883046; CAE84004.1; -; Genomic_DNA.
DR PIR; JN0869; JN0869.
DR RefSeq; NP_542947.1; NM_080769.2.
DR RefSeq; XP_017457036.1; XM_017601547.1.
DR AlphaFoldDB; Q06332; -.
DR SMR; Q06332; -.
DR STRING; 10116.ENSRNOP00000001111; -.
DR GlyCosmos; Q06332; 1 site, No reported glycans.
DR GlyGen; Q06332; 1 site.
DR PaxDb; 10116-ENSRNOP00000001111; -.
DR Ensembl; ENSRNOT00000001111.3; ENSRNOP00000001111.1; ENSRNOG00000000838.4.
DR Ensembl; ENSRNOT00055013228; ENSRNOP00055010604; ENSRNOG00055007875.
DR Ensembl; ENSRNOT00060007870; ENSRNOP00060005914; ENSRNOG00060004710.
DR Ensembl; ENSRNOT00065053840; ENSRNOP00065044279; ENSRNOG00065031221.
DR GeneID; 25008; -.
DR KEGG; rno:25008; -.
DR UCSC; RGD:3020; rat.
DR AGR; RGD:3020; -.
DR CTD; 4049; -.
DR RGD; 3020; Lta.
DR eggNOG; ENOG502S956; Eukaryota.
DR GeneTree; ENSGT01060000248544; -.
DR InParanoid; Q06332; -.
DR OMA; MEGECKV; -.
DR OrthoDB; 2909163at2759; -.
DR PhylomeDB; Q06332; -.
DR TreeFam; TF332169; -.
DR Reactome; R-RNO-5668541; TNFR2 non-canonical NF-kB pathway.
DR Reactome; R-RNO-5669034; TNFs bind their physiological receptors.
DR Reactome; R-RNO-5676594; TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway.
DR PRO; PR:Q06332; -.
DR Proteomes; UP000002494; Chromosome 20.
DR Bgee; ENSRNOG00000000838; Expressed in Ammon's horn and 7 other cell types or tissues.
DR Genevisible; Q06332; RN.
DR GO; GO:0005615; C:extracellular space; IDA:RGD.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR GO; GO:0005164; F:tumor necrosis factor receptor binding; IEA:InterPro.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; ISO:RGD.
DR GO; GO:0006959; P:humoral immune response; ISO:RGD.
DR GO; GO:0048535; P:lymph node development; ISO:RGD.
DR GO; GO:0048147; P:negative regulation of fibroblast proliferation; IDA:RGD.
DR GO; GO:0043065; P:positive regulation of apoptotic process; IMP:RGD.
DR GO; GO:0002876; P:positive regulation of chronic inflammatory response to antigenic stimulus; ISO:RGD.
DR GO; GO:0060252; P:positive regulation of glial cell proliferation; IMP:RGD.
DR GO; GO:0002925; P:positive regulation of humoral immune response mediated by circulating immunoglobulin; ISO:RGD.
DR GO; GO:0032729; P:positive regulation of type II interferon production; ISO:RGD.
DR GO; GO:0001666; P:response to hypoxia; IEP:RGD.
DR GO; GO:0032496; P:response to lipopolysaccharide; IEP:RGD.
DR GO; GO:0007584; P:response to nutrient; IEP:RGD.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IEP:RGD.
DR CDD; cd00184; TNF; 1.
DR Gene3D; 2.60.120.40; -; 1.
DR InterPro; IPR006053; TNF.
DR InterPro; IPR002960; TNF_beta.
DR InterPro; IPR021184; TNF_CS.
DR InterPro; IPR006052; TNF_dom.
DR InterPro; IPR008983; Tumour_necrosis_fac-like_dom.
DR PANTHER; PTHR11471:SF31; LYMPHOTOXIN-ALPHA; 1.
DR PANTHER; PTHR11471; TUMOR NECROSIS FACTOR FAMILY MEMBER; 1.
DR Pfam; PF00229; TNF; 1.
DR PRINTS; PR01234; TNECROSISFCT.
DR PRINTS; PR01236; TNFBETA.
DR SMART; SM00207; TNF; 1.
DR SUPFAM; SSF49842; TNF-like; 1.
DR PROSITE; PS00251; TNF_1; 1.
DR PROSITE; PS50049; TNF_2; 1.
PE 3: Inferred from homology;
KW Cytokine; Glycoprotein; Membrane; Reference proteome; Secreted; Signal.
FT SIGNAL 1..33
FT /evidence="ECO:0000250"
FT CHAIN 34..202
FT /note="Lymphotoxin-alpha"
FT /id="PRO_0000034471"
FT CARBOHYD 93
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 202 AA; 22112 MW; A56963CE33AE0C08 CRC64;
MTPLGRLHLL RVLSTPPVFL LGLLLALPLG AQGLSGVRFS ASRTAHQPPQ KHLTHGLLKP
AAHLVGYPSK QNSLLWRANT DRAFLRHGFS LNNNSLLIPT SGLYFVYSQV VFSGESCSPR
AIPTPIYLAH EVQLFSSQYP FHVPLLSAQK SVYPGLQGPW VRSMYQGAVF LLSKGDQLST
HTDGISHLHF SPSTVFFGAF AL
//
