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Database: UniProt/SWISS-PROT
Entry: TRUB_ARTS2
LinkDB: TRUB_ARTS2
Original site: TRUB_ARTS2 
ID   TRUB_ARTS2              Reviewed;         306 AA.
AC   A0JUU8;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   08-MAY-2019, entry version 80.
DE   RecName: Full=tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01080};
DE            EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE            Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01080};
GN   Name=truB {ECO:0000255|HAMAP-Rule:MF_01080};
GN   OrderedLocusNames=Arth_1424;
OS   Arthrobacter sp. (strain FB24).
OC   Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Arthrobacter.
OX   NCBI_TaxID=290399;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FB24;
RX   PubMed=24501649; DOI=10.4056/sigs.4438185;
RA   Nakatsu C.H., Barabote R., Thompson S., Bruce D., Detter C.,
RA   Brettin T., Han C., Beasley F., Chen W., Konopka A., Xie G.;
RT   "Complete genome sequence of Arthrobacter sp. strain FB24.";
RL   Stand. Genomic Sci. 9:106-116(2013).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-
CC       55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_01080}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-
CC         COMP:10102, ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01080};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family.
CC       Type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_01080}.
DR   EMBL; CP000454; ABK02818.1; -; Genomic_DNA.
DR   SMR; A0JUU8; -.
DR   STRING; 290399.Arth_1424; -.
DR   EnsemblBacteria; ABK02818; ABK02818; Arth_1424.
DR   KEGG; art:Arth_1424; -.
DR   eggNOG; ENOG4105D0T; Bacteria.
DR   eggNOG; COG0130; LUCA.
DR   HOGENOM; HOG000231225; -.
DR   KO; K03177; -.
DR   OMA; ELQFIRW; -.
DR   Proteomes; UP000000754; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   CDD; cd02573; PseudoU_synth_EcTruB; 1.
DR   Gene3D; 2.30.130.10; -; 1.
DR   HAMAP; MF_01080; TruB_bact; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR015947; PUA-like_sf.
DR   InterPro; IPR036974; PUA_sf.
DR   InterPro; IPR015225; tRNA_psdUridine_synth_fam2_C.
DR   InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR   InterPro; IPR032819; TruB_C.
DR   PANTHER; PTHR13767; PTHR13767; 1.
DR   Pfam; PF09142; TruB_C; 1.
DR   Pfam; PF16198; TruB_C_2; 1.
DR   Pfam; PF01509; TruB_N; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   SUPFAM; SSF88697; SSF88697; 1.
DR   TIGRFAMs; TIGR00431; TruB; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Isomerase; Reference proteome; tRNA processing.
FT   CHAIN         1    306       tRNA pseudouridine synthase B.
FT                                /FTId=PRO_1000084544.
FT   ACT_SITE     39     39       Nucleophile. {ECO:0000255|HAMAP-
FT                                Rule:MF_01080}.
SQ   SEQUENCE   306 AA;  31873 MW;  13BD2F21F300E941 CRC64;
     MLSGLVIVDK PQGWTSHDVV GRMRRLAGTR KVGHAGTLDP MATGVLVLGI NKATRLLTYI
     VGTSKTYTAT IRLGESTVTD DAEGEVVSSH SAAAVTEGAI RAAVAALTGE IQQVPSSVSA
     IKVNGERAYA RVRSGEDVKL AARPVTIHRF DVHAVRPERA GAVLDVDVTV ECSSGTYIRA
     LARDLGEALG TGGHLTALRR TQVGPYTLDQ ARTLEQLAEE LEVLEMSQAA RALMPNRELS
     EDEATEISFG RRIAAGAGAG TPEAATADNP AAAFAPDGSL VALLADAGGY AKPVLVFAPS
     NEQPGK
//
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