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Database: UniProt/SWISS-PROT
Entry: TRUB_NOCFA
LinkDB: TRUB_NOCFA
Original site: TRUB_NOCFA 
ID   TRUB_NOCFA              Reviewed;         306 AA.
AC   Q5YSE0;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   08-MAY-2019, entry version 92.
DE   RecName: Full=tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01080};
DE            EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE            Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01080};
GN   Name=truB {ECO:0000255|HAMAP-Rule:MF_01080};
GN   OrderedLocusNames=NFA_40530;
OS   Nocardia farcinica (strain IFM 10152).
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Nocardia.
OX   NCBI_TaxID=247156;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IFM 10152;
RX   PubMed=15466710; DOI=10.1073/pnas.0406410101;
RA   Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K.,
RA   Shiba T., Hattori M.;
RT   "The complete genomic sequence of Nocardia farcinica IFM 10152.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-
CC       55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_01080}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-
CC         COMP:10102, ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01080};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family.
CC       Type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_01080}.
DR   EMBL; AP006618; BAD58901.1; -; Genomic_DNA.
DR   RefSeq; WP_011210586.1; NC_006361.1.
DR   SMR; Q5YSE0; -.
DR   STRING; 247156.NFA_40530; -.
DR   EnsemblBacteria; BAD58901; BAD58901; NFA_40530.
DR   KEGG; nfa:NFA_40530; -.
DR   eggNOG; ENOG4105D0T; Bacteria.
DR   eggNOG; COG0130; LUCA.
DR   KO; K03177; -.
DR   OMA; ELQFIRW; -.
DR   BioCyc; NFAR247156:NFA_RS20145-MONOMER; -.
DR   Proteomes; UP000006820; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   CDD; cd02573; PseudoU_synth_EcTruB; 1.
DR   Gene3D; 2.30.130.10; -; 1.
DR   HAMAP; MF_01080; TruB_bact; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR015947; PUA-like_sf.
DR   InterPro; IPR036974; PUA_sf.
DR   InterPro; IPR015225; tRNA_psdUridine_synth_fam2_C.
DR   InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR   InterPro; IPR032819; TruB_C.
DR   PANTHER; PTHR13767; PTHR13767; 1.
DR   Pfam; PF09142; TruB_C; 1.
DR   Pfam; PF16198; TruB_C_2; 1.
DR   Pfam; PF01509; TruB_N; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   SUPFAM; SSF88697; SSF88697; 1.
DR   TIGRFAMs; TIGR00431; TruB; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Isomerase; Reference proteome; tRNA processing.
FT   CHAIN         1    306       tRNA pseudouridine synthase B.
FT                                /FTId=PRO_0000121877.
FT   ACT_SITE     51     51       Nucleophile. {ECO:0000255|HAMAP-
FT                                Rule:MF_01080}.
SQ   SEQUENCE   306 AA;  32184 MW;  6FE47ABEEBEB3C2B CRC64;
     MGERSAPTRR VDPLGGLLVV DKDGGMTSHD VVARCRKILG TRKIGHAGTL DPMATGVLVL
     GVERATKLLG LLTLTTKAYT ATIRLGSATT TDDAEGEVLT TVPAGHLGDA EVAAGVAALT
     GDIQQVPATV SAIKIGGERA YARHRAGEQV ELAARPVTVS RFEVLARRDV AGFVDLDVVV
     ECSSGTYVRA LARDLGAALG VGGHLTALRR TRVGPFTLDH ARTLDALADE PRLNLDMDEA
     VRIAFPHRAI DAREAESLRD GRWLDPVGIP GVYAALTADG TAIALLEEKG KRASPVFVVR
     PRGLVD
//
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