GenomeNet

Database: UniProt/SWISS-PROT
Entry: UNC4_CAEEL
LinkDB: UNC4_CAEEL
Original site: UNC4_CAEEL 
ID   UNC4_CAEEL              Reviewed;         252 AA.
AC   P29506;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   13-NOV-2019, entry version 152.
DE   RecName: Full=Homeobox protein unc-4;
DE   AltName: Full=Homeobox protein ceh-4;
DE   AltName: Full=Uncoordinated protein 4;
GN   Name=unc-4; Synonyms=ceh-4; ORFNames=F26C11.2;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 37-220, AND FUNCTION.
RC   STRAIN=Bristol N2;
RX   PubMed=1347150; DOI=10.1038/355841a0;
RA   Miller D.M. III, Shen M.M., Shamu C.E., Buerglin T.R., Ruvkun G.,
RA   Dubois M.L., Ghee M., Wilson L.;
RT   "C. elegans unc-4 gene encodes a homeodomain protein that determines
RT   the pattern of synaptic input to specific motor neurons.";
RL   Nature 355:841-845(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 134-147.
RX   PubMed=2571091; DOI=10.1038/341239a0;
RA   Buerglin T.R., Finney M., Coulson A., Ruvkun G.;
RT   "Caenorhabditis elegans has scores of homoeobox-containing genes.";
RL   Nature 341:239-243(1989).
RN   [4]
RP   FUNCTION, INTERACTION WITH UNC-37, MUTAGENESIS OF LEU-121, AND
RP   DISRUPTION PHENOTYPE.
RX   PubMed=7904971;
RA   Miller D.M. III, Niemeyer C.J., Chitkara P.;
RT   "Dominant unc-37 mutations suppress the movement defect of a
RT   homeodomain mutation in unc-4, a neural specificity gene in
RT   Caenorhabditis elegans.";
RL   Genetics 135:741-753(1993).
RN   [5]
RP   FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX   PubMed=7555714;
RA   Miller D.M. III, Niemeyer C.J.;
RT   "Expression of the unc-4 homeoprotein in Caenorhabditis elegans motor
RT   neurons specifies presynaptic input.";
RL   Development 121:2877-2886(1995).
RN   [6]
RP   FUNCTION.
RX   PubMed=9165118;
RA   Pflugrad A., Meir J.Y.-J., Barnes T.M., Miller D.M. III;
RT   "The Groucho-like transcription factor UNC-37 functions with the
RT   neural specificity gene unc-4 to govern motor neuron identity in C.
RT   elegans.";
RL   Development 124:1699-1709(1997).
RN   [7]
RP   FUNCTION, INTERACTION WITH UNC-37, AND MUTAGENESIS OF PHE-174;
RP   GLY-188; ARG-190 AND ARG-197.
RX   PubMed=10557206; DOI=10.1101/gad.13.21.2774;
RA   Winnier A.R., Meir J.Y.-J., Ross J.M., Tavernarakis N., Driscoll M.,
RA   Ishihara T., Katsura I., Miller D.M. III;
RT   "UNC-4/UNC-37-dependent repression of motor neuron-specific genes
RT   controls synaptic choice in Caenorhabditis elegans.";
RL   Genes Dev. 13:2774-2786(1999).
RN   [8]
RP   FUNCTION.
RX   PubMed=11245684; DOI=10.1523/jneurosci.21-06-02001.2001;
RA   Lickteig K.M., Duerr J.S., Frisby D.L., Hall D.H., Rand J.B.,
RA   Miller D.M. III;
RT   "Regulation of neurotransmitter vesicles by the homeodomain protein
RT   UNC-4 and its transcriptional corepressor UNC-37/groucho in
RT   Caenorhabditis elegans cholinergic motor neurons.";
RL   J. Neurosci. 21:2001-2014(2001).
RN   [9]
RP   FUNCTION.
RX   PubMed=17289921; DOI=10.1101/gad.1502107;
RA   Von Stetina S.E., Fox R.M., Watkins K.L., Starich T.A., Shaw J.E.,
RA   Miller D.M. III;
RT   "UNC-4 represses CEH-12/HB9 to specify synaptic inputs to VA motor
RT   neurons in C. elegans.";
RL   Genes Dev. 21:332-346(2007).
CC   -!- FUNCTION: Transcription factor that regulates synaptic
CC       specificity. Determines the pattern of synaptic input to VA motor
CC       neurons. Acts together with unc-37 by repressing the expression of
CC       VB-specific genes such as ceh-12, thereby preventing the adoption
CC       of VB motor neurons. Has no role in axonal guidance or outgrowth.
CC       {ECO:0000269|PubMed:10557206, ECO:0000269|PubMed:11245684,
CC       ECO:0000269|PubMed:1347150, ECO:0000269|PubMed:17289921,
CC       ECO:0000269|PubMed:7555714, ECO:0000269|PubMed:7904971,
CC       ECO:0000269|PubMed:9165118}.
CC   -!- SUBUNIT: Interacts with unc-37. {ECO:0000269|PubMed:10557206,
CC       ECO:0000269|PubMed:7904971}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Specifically expressed in all A-type motor
CC       neurons. The A-type motor neurons class includes 24 motor neurons;
CC       9 DA, 3 SAB motor neurons that are generated in the embryo and 12
CC       VAs arise after hatching in the first larval stage.
CC       {ECO:0000269|PubMed:7555714}.
CC   -!- DEVELOPMENTAL STAGE: Most abundant during embryonic and larval
CC       development. {ECO:0000269|PubMed:7555714}.
CC   -!- DISRUPTION PHENOTYPE: Worms are unable to crawl backwards because
CC       VA motor neurons are miswired with synaptic connections normally
CC       reserved for their sister cells, the VB motor neurons.
CC       {ECO:0000269|PubMed:7904971}.
CC   -!- SIMILARITY: Belongs to the paired homeobox family. Unc-4
CC       subfamily. {ECO:0000305}.
DR   EMBL; Z47072; CAA87368.1; -; Genomic_DNA.
DR   EMBL; X64904; CAA46108.1; -; mRNA.
DR   PIR; T21388; T21388.
DR   RefSeq; NP_496138.1; NM_063737.1.
DR   SMR; P29506; -.
DR   STRING; 6239.F26C11.2; -.
DR   PaxDb; P29506; -.
DR   EnsemblMetazoa; F26C11.2.1; F26C11.2.1; WBGene00006744.
DR   GeneID; 174544; -.
DR   KEGG; cel:CELE_F26C11.2; -.
DR   UCSC; F26C11.2; c. elegans.
DR   CTD; 32757; -.
DR   WormBase; F26C11.2; CE01560; WBGene00006744; unc-4.
DR   eggNOG; KOG0490; Eukaryota.
DR   eggNOG; ENOG410YIJ3; LUCA.
DR   GeneTree; ENSGT00940000167841; -.
DR   HOGENOM; HOG000016205; -.
DR   InParanoid; P29506; -.
DR   KO; K09328; -.
DR   OMA; FPITQPG; -.
DR   OrthoDB; 1014909at2759; -.
DR   PhylomeDB; P29506; -.
DR   SignaLink; P29506; -.
DR   PRO; PR:P29506; -.
DR   Proteomes; UP000001940; Chromosome II.
DR   Bgee; WBGene00006744; Expressed in 4 organ(s), highest expression level in multi-cellular organism.
DR   GO; GO:0030424; C:axon; IDA:WormBase.
DR   GO; GO:0005623; C:cell; IEA:GOC.
DR   GO; GO:0005634; C:nucleus; IDA:WormBase.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0040011; P:locomotion; IMP:WormBase.
DR   GO; GO:0007400; P:neuroblast fate determination; IMP:WormBase.
DR   GO; GO:0050770; P:regulation of axonogenesis; IMP:WormBase.
DR   GO; GO:0050803; P:regulation of synapse structure or activity; IMP:WormBase.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR   GO; GO:0007416; P:synapse assembly; IMP:WormBase.
DR   GO; GO:0008039; P:synaptic target recognition; IMP:UniProtKB.
DR   GO; GO:0048489; P:synaptic vesicle transport; IMP:UniProtKB.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   Pfam; PF00046; Homeodomain; 1.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Developmental protein; Differentiation;
KW   DNA-binding; Homeobox; Neurogenesis; Nucleus; Reference proteome;
KW   Repressor; Transcription; Transcription regulation.
FT   CHAIN         1    252       Homeobox protein unc-4.
FT                                /FTId=PRO_0000049347.
FT   DNA_BIND     88    147       Homeobox. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00108}.
FT   MUTAGEN     121    121       L->F: In e2322ts; unable to crawl
FT                                backwards. {ECO:0000269|PubMed:7904971}.
FT   MUTAGEN     174    174       F->E: In wdEx65; unable to crawl
FT                                backwards. Abolishes interaction with
FT                                unc-37. {ECO:0000269|PubMed:10557206}.
FT   MUTAGEN     188    188       G->D,S: In e26/e2307; unable to crawl
FT                                backwards. Abolishes interaction with
FT                                unc-37. {ECO:0000269|PubMed:10557206}.
FT   MUTAGEN     190    190       R->Q: In e2323; unable to crawl
FT                                backwards. Abolishes interaction with
FT                                unc-37. {ECO:0000269|PubMed:10557206}.
FT   MUTAGEN     197    197       R->K: In e26/e2307; unable to crawl
FT                                backwards. Abolishes interaction with
FT                                unc-37. {ECO:0000269|PubMed:10557206}.
SQ   SEQUENCE   252 AA;  28354 MW;  89CF28B1AC69031A CRC64;
     MIGALHACVD AEPKIINDIW ADFWKSQINS VLLNPSDGSE TYLASDNGKS TSSREQSTSP
     DDDNLLMNED DGIALEDDND TGESAAKRRR TRTNFSGWQL EELESAFEAS HYPDVFMREA
     LAMRLDLLES RVQVWFQNRR AKWRKREQNR NGSSEIKKDD GEQMETKALP TFPFSIDSIL
     AVSRVPRGRR PNAKYPRVQA CKNLSPFMIP LFPITQPGGN VIREKSPPLP TQQSQIVATN
     ALTTVAELLK SV
//
DBGET integrated database retrieval system