KEGG   PATHWAY: spo03030Help
Entry
spo03030                    Pathway                                

Name
DNA replication - Schizosaccharomyces pombe (fission yeast)
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
09120 Genetic Information Processing; 09124 Replication and repair
BRITE hierarchy
Pathway map
spo03030  DNA replication
spo03030

Ortholog table
Module
spo_M00261  DNA polymerase alpha / primase complex [PATH:spo03030]
spo_M00262  DNA polymerase delta complex [PATH:spo03030]
spo_M00263  DNA polymerase epsilon complex [PATH:spo03030]
spo_M00285  MCM complex [PATH:spo03030]
spo_M00288  RPA complex [PATH:spo03030]
spo_M00289  RF-C complex [PATH:spo03030]
Organism
Schizosaccharomyces pombe (fission yeast) [GN:spo]
Gene
SPBC336.06c  rnh1; ribonuclease H Rnh1 (predicted) [KO:K03469] [EC:3.1.26.4]
SPBC660.13c  ssb1; DNA replication factor A subunit Ssb1 [KO:K07466]
SPBC16D10.09  pcn1; DNA polymerase delta processivity factor [KO:K04802]
SPBC16D10.04c  dna2; DNA replication endonuclease-helicase Dna2 [KO:K10742] [EC:3.6.4.12]
SPAC3G6.06c  rad2; FEN-1 endonuclease Rad2 [KO:K04799] [EC:3.-.-.-]
SPAC20G8.01  cdc17; ATP-dependent DNA ligase Cdc17 [KO:K10747] [EC:6.5.1.7 6.5.1.6 6.5.1.1]
SPBC713.06  adl1; DNA ligase (predicted) [KO:K10747] [EC:6.5.1.7 6.5.1.6 6.5.1.1]
SPAC3H5.06c  pol1; DNA polymerase alpha catalytic subunit [KO:K02320] [EC:2.7.7.7]
SPCC553.09c  spb70; DNA polymerase alpha B-subunit [KO:K02321]
SPAC6B12.10c  spp1; DNA primase catalytic subunit Spp1 [KO:K02684] [EC:2.7.7.-]
SPBC17D11.06  spp2; DNA primase large subunit Spp2 [KO:K02685] [EC:2.7.7.-]
SPBC336.04  cdc6; DNA polymerase delta catalytic subunit Cdc6 [KO:K02327] [EC:2.7.7.7]
SPAC27E2.05  cdc1; DNA polymerase delta small subunit Cdc1 [KO:K02328]
SPBC1734.02c  cdc27; DNA polymerase delta subunit Cdc27 [KO:K03504]
SPBC12D12.02c  cdm1; DNA polymerase delta subunit Cdm1 [KO:K03505]
SPBC25H2.13c  cdc20; DNA polymerase epsilon catalytic subunit Pol2 [KO:K02324] [EC:2.7.7.7]
SPBP8B7.14c  dpb2; DNA polymerase epsilon catalytic subunit B, Dpb2 [KO:K02325] [EC:2.7.7.7]
SPBC3D6.09  dpb4; DNA polymerase epsilon subunit Dpb4 [KO:K02326] [EC:2.7.7.7]
SPCC16C4.22  histone-like transcription factor family (CBF/NF-Y) [KO:K03506] [EC:2.7.7.7]
SPBC4.04c  mcm2; MCM complex subunit Mcm2 [KO:K02540] [EC:3.6.4.12]
SPCC1682.02c  mcm3; MCM complex subunit Mcm3 [KO:K02541] [EC:3.6.4.12]
SPCC16A11.17  mcm4; MCM complex subunit Mcm4/Cdc21 [KO:K02212] [EC:3.6.4.12]
SPAC1B2.05  mcm5; MCM complex subunit Mcm5 [KO:K02209] [EC:3.6.4.12]
SPBC211.04c  mcm6; MCM complex subunit Mcm6 [KO:K02542] [EC:3.6.4.12]
SPBC25D12.03c  mcm7; MCM complex subunit Mcm7 [KO:K02210] [EC:3.6.4.12]
SPCC1753.01c  ssb2; single-stranded DNA binding protein Ssb2 [KO:K10739]
SPCC23B6.05c  ssb3; DNA replication factor A subunit Ssb3 [KO:K10740]
SPBC23E6.07c  rfc1; DNA replication factor C complex subunit Rfc1 [KO:K10754]
SPAC23D3.02  rfc2; DNA replication factor C complex subunit Rfc2 [KO:K10755]
SPAC1687.03c  rfc4; DNA replication factor C complex subunit Rfc4 (predicted) [KO:K10755]
SPAC27E2.10c  rfc3; DNA replication factor C complex subunit Rfc3 [KO:K10756]
SPBC83.14c  rfc5; DNA replication factor C complex subunit Rfc5 (predicted) [KO:K10756]
SPAC4G9.02  rnh201; ribonuclease H2 complex subunit Rnh201 (predicted) [KO:K10743] [EC:3.1.26.4]
SPBC1347.08c  ribonuclease H2 complex subunit (predicted) [KO:K10744]
SPAC12B10.15c  ribonuclease H2 complex subunit (predicted) [KO:K10745]
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
DOI:10.1016/S0092-8674(94)90362-X
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
DOI:10.1146/annurev.biochem.67.1.721
KO pathway
ko03030   

DBGET integrated database retrieval system