ID A0A010YXL8_9ACTN Unreviewed; 1210 AA.
AC A0A010YXL8;
DT 11-JUN-2014, integrated into UniProtKB/TrEMBL.
DT 11-JUN-2014, sequence version 1.
DT 24-JAN-2024, entry version 45.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=CryarDRAFT_0996 {ECO:0000313|EMBL:EXG79943.1};
OS Cryptosporangium arvum DSM 44712.
OC Bacteria; Actinomycetota; Actinomycetes; Cryptosporangiales;
OC Cryptosporangiaceae; Cryptosporangium.
OX NCBI_TaxID=927661 {ECO:0000313|EMBL:EXG79943.1, ECO:0000313|Proteomes:UP000021053};
RN [1] {ECO:0000313|EMBL:EXG79943.1, ECO:0000313|Proteomes:UP000021053}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44712 {ECO:0000313|EMBL:EXG79943.1,
RC ECO:0000313|Proteomes:UP000021053};
RG DOE Joint Genome Institute;
RA Eisen J., Huntemann M., Han J., Chen A., Kyrpides N., Mavromatis K.,
RA Markowitz V., Palaniappan K., Ivanova N., Schaumberg A., Pati A.,
RA Liolios K., Nordberg H.P., Cantor M.N., Hua S.X., Woyke T.;
RL Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EXG79943.1}.
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DR EMBL; JFBT01000001; EXG79943.1; -; Genomic_DNA.
DR RefSeq; WP_035848712.1; NZ_KK073874.1.
DR AlphaFoldDB; A0A010YXL8; -.
DR PATRIC; fig|927661.3.peg.979; -.
DR HOGENOM; CLU_005122_2_2_11; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000021053; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000021053}.
FT DOMAIN 675..836
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 861..1011
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1210 AA; 131113 MW; A98BB75474A294A8 CRC64;
MSLSGLLDAS LADPALGQIA RRAAGDAAGE LDLTAPEALR PFVVAALAAA RAKAGSAAPV
LAVTATGREA DDLAAALTCL LPPDRIAVYP AWETLPHEKL SPRADTIGRR LAVLRRLAHP
STDDPTTGPL DVVIAPVRSV LQPQLAGLGE LEPVGLQAGD TAPLDAVVER LVSIAYARVD
LVEKRGEFAV RGGILDVFPP TEEHPVRVEF WGDEVEEVRY FAVADQRSLE IAAHGLWAPP
CRELLLTPEV RERAAQLAKD HPALAEMLDK VADGIPVEGM ESLTPALLEG SGQLELLVEA
VPAGTHVLLC DPERIRARAR DLVATSAEFL EASWAAAAGG GEAPIDLGAA AFRSLADVRS
TGTGRGLPWW TVAPFGVSAA GSASAADFDD DAIDVAIPAV ADPGVPRDSL TVEAKSVDGF
RGDTGRAVEE LRQRVAEGWR AALVFEGPGS AQRAAEMLRE ADLGAQLVVD VPTAPEARSV
LVTTGQLAGG FLLESAQLVV FTESDLTGAR SGSGRERGRM PSRRRNAVDP LQLQPGDYVV
HEQHGVGKYI EMIKRTINGG EREYLVLEYA PSKRGHPGDR LFVPTDSLEQ LTRYVGGESP
ALHKMGGADW QKAKGRARKA VKEIAGELIR LYAARTSTKG FTFSADTPWQ RELEDAFPYI
ETPDQLVAIN EVKADMEKPY PMDRVICGDV GYGKTEIAVR AAFKAVQDGK QVAVLVPTTI
LAKQHFLTFS ERMAQFPVKI AQLSRFQTDG EAAETVAKLA TGEVDIVVGT HRLLQASTRF
KDLGLVIVDE EQRFGVEHKE QLKQMRTAVD VLTMSATPIP RTLEMSLTGI REMSTILTPP
EERHPVLTFV GPYEERQIGA AIRRELLREG QVFYLHNRVE SIERAAARLR SMVPEARIAV
AHGQMNEDAL ERVMLGFDEK EYDVLVSTTI VESGLDIPNA NTLIVERADV LGLSQLHQIR
GRVGRGRERA YAYFLYPPER PLTETAHDRL ATIAQNSDLG AGMAVAMKDL EIRGSGNLLG
GEQSGHIANV GFDLYVRLVG EAVAEYKGER TEIESEVKVD LPVDAHLPHD YIPGERLRLE
AYRKLAGVTT SEELAQVREE IRDRYGNPPE PVENLFAVAA FRVKARAATI TEVSLQGRQV
RFAPLRLPES KVMRLQRLYP GAMYKQAVDV VLVPRPSTAR VGGQPLRDTA LLDWAAGFLD
AMAPEPARAP
//
