ID A0A016WA59_9BILA Unreviewed; 2308 AA.
AC A0A016WA59;
DT 11-JUN-2014, integrated into UniProtKB/TrEMBL.
DT 11-JUN-2014, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=Reverse transcriptase domain-containing protein {ECO:0000259|PROSITE:PS50878};
GN Name=Acey_s0960.g3219 {ECO:0000313|EMBL:EYC35908.1};
GN ORFNames=Y032_0960g3219 {ECO:0000313|EMBL:EYC35908.1};
OS Ancylostoma ceylanicum.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Strongyloidea; Ancylostomatidae;
OC Ancylostomatinae; Ancylostoma.
OX NCBI_TaxID=53326 {ECO:0000313|EMBL:EYC35908.1, ECO:0000313|Proteomes:UP000024635};
RN [1] {ECO:0000313|Proteomes:UP000024635}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=HY135 {ECO:0000313|Proteomes:UP000024635};
RX PubMed=25730766; DOI=10.1038/ng.3237;
RA Schwarz E.M., Hu Y., Antoshechkin I., Miller M.M., Sternberg P.W.,
RA Aroian R.V.;
RT "The genome and transcriptome of the zoonotic hookworm Ancylostoma
RT ceylanicum identify infection-specific gene families.";
RL Nat. Genet. 47:416-422(2015).
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR634016-3};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRSR:PIRSR634016-3};
CC -!- SIMILARITY: Belongs to the peptidase M1 family.
CC {ECO:0000256|ARBA:ARBA00010136}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EYC35908.1}.
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DR EMBL; JARK01000560; EYC35908.1; -; Genomic_DNA.
DR STRING; 53326.A0A016WA59; -.
DR Proteomes; UP000024635; Unassembled WGS sequence.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09601; M1_APN-Q_like; 2.
DR CDD; cd01650; RT_nLTR_like; 1.
DR Gene3D; 1.25.50.20; -; 1.
DR Gene3D; 2.60.40.1910; -; 1.
DR Gene3D; 3.30.70.270; -; 1.
DR Gene3D; 3.60.10.10; Endonuclease/exonuclease/phosphatase; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 3.
DR Gene3D; 2.60.40.1730; tricorn interacting facor f3 domain; 2.
DR InterPro; IPR045357; Aminopeptidase_N-like_N.
DR InterPro; IPR042097; Aminopeptidase_N-like_N_sf.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
DR InterPro; IPR024571; ERAP1-like_C_dom.
DR InterPro; IPR034016; M1_APN-typ.
DR InterPro; IPR001930; Peptidase_M1.
DR InterPro; IPR014782; Peptidase_M1_dom.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR000477; RT_dom.
DR PANTHER; PTHR11533:SF293; AMINOPEPTIDASE-2-RELATED; 1.
DR PANTHER; PTHR11533; PROTEASE M1 ZINC METALLOPROTEASE; 1.
DR Pfam; PF11838; ERAP1_C; 1.
DR Pfam; PF01433; Peptidase_M1; 2.
DR Pfam; PF17900; Peptidase_M1_N; 2.
DR Pfam; PF00078; RVT_1; 1.
DR PRINTS; PR00756; ALADIPTASE.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF56219; DNase I-like; 1.
DR SUPFAM; SSF63737; Leukotriene A4 hydrolase N-terminal domain; 2.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 2.
DR PROSITE; PS50878; RT_POL; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR634016-3};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000024635};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRSR:PIRSR634016-3}.
FT DOMAIN 354..608
FT /note="Reverse transcriptase"
FT /evidence="ECO:0000259|PROSITE:PS50878"
FT ACT_SITE 1078
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-1"
FT BINDING 1077
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 1081
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT SITE 1138
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-4"
SQ SEQUENCE 2308 AA; 262582 MW; 2B078E79D39E4FC6 CRC64;
MDLEKFYRQE HTFYKVIVGD FNTKIGPRRT PEELQIGTHG LQWNEQGERL SEFIMTTKTI
HGNSQFQKPT SLRWTWESPG GEYHNEIDHI IVNRRFCLTD VGVVPKFYTG SDHRLLRARF
FFSRKGEKAA KYKKRSPKPT INWDLFTTLA GFWEDTVVDN TDEEYERLIQ HLRDSAKKAE
GSRTTKRRLS HETLELIRQR GAARAAGNYQ LTSELARRCR EAIKEDLKER RAAVLAEAAE
AGRSIRNTRR DFANRKTKMT ALRRPDGTIT SSRRVMEKVI YDFYSDLFDS HVHLPPYHLR
EDGYLIPSVL PSEVRHAIKS VKNRTAPGPD RIRPEHLKNL PTALVNTLAR LFTRYLSECK
VPSQWKTSRT VLLYKKGDPQ DIGNYRPICL LSVVYKLFTR VILNRIERTL DEGQPCEQAG
FRKGFSAIDH IHTVTRLVEV SREYEMPLCL TFIDLKKAFD TVETEAVLEA LGNQGVPTQY
IRIFRELYSN FTTRISPFYD DITIDVRRGV RQGDTVSPKL FTATLEDVMR RLEWDNMGVR
VDGRLLHHLR FADDIVLITP SISQAERMLA DFDDACGKIG LQLNLTKTMF MRNGWVPDAP
FSLNGTTISE CSSYVYLGRE VNMMNDLAPE LGRRKRAAWG AYKSIEDVVK RTKNTRLSHL
FNTTVLPDLT YASETWAVPK QDENAVSVIE RSIERVMLGM TRLTQQLIVD RLQSSMDRVR
LVDVLLLAVT LLGHFTSALP HHQLFIPGDP RIQDIDRDDG PEDDIPARIP DDISVTEYYI
KIQPYYPAPG IQLDKGRNMT FDGSVSMSVK IVKPTSEIIL NAANLKIVSI ELTDFLKRPV
VIKEMRLNNE TEQLSFVLQK RPRVGSVFVL AIKYTGKINP YYDAGLYYTY YEDSEGEVHW
MVATQLAPFS ARTVFPCMDE PAFKAIFHVE LVYPSSHVAL GNMKETPPVD LGNGWSKVSF
PPTPVMSTYL VAFSSGPYVS HSVINKDGTL VRSWGWTGQE NFLKFSAETA GECLHQMGLY
TNIKFPLEKC DHLGLPEFLA GAMENFGLIV YKYQFISFNP DAMTTLDKIG AALVICHEVS
HQWFGDLVTA ECKDSLLGSF QEAATVRIDR EKALARDGSA YTHPLIAVDG PHFDPITYEK
GQMLLRMLAD TIDEEVLRSG LQNYLRAHQY STASHWDLWS GLTEVSNDAG VRGWHGPLNV
TELMEPYALQ SSFPVINVHA GKTGISFSQE RFNDISTQSS SPWNYTWIIP LRTAEYSSPG
TTIRWLVPDR FEEPLEHSSQ ATNRWHVVSH SSATYGRVVY DENSLHALLQ KIAAVDVPVG
VKIALIGDEV AMIKRNKSIK QPYSYHRLLD ILATMFNTPS KEDPSSQLVD IALPQMEFFA
NLLRDSIDAP LIEQLFGLVF GKAYKSEIWD APSSWNADAM KNIFLPYAVR YSIGDASSTA
QKFFNQIAEN CKSAESNNGS AWCSKVPNDI HRAAYCGAAK YDNNLGANFA RLLFLYNGEV
KTNPYYYQEY TALLEGMACT ERAPQLKTLI RLLIASPHRP SMIFGWLKTN PKASEALYLY
LKTKSDSVLR YTGLSYYLDA MVYNWRSERR LRQFNELHKS LLPKLNNKQK DAFAKFEKKI
RDNIEWSKQH LPSIMRWMYD NLVVVGKAPW RKSLPGIISP ERYDVEITPY IPGSGKYHFS
KNMTFDGSVK MKFTVTQETS EIVVNAHRMV IDVDSIVVKD SHNSNIEVSA VDIAKDYEHG
ILKIPLATKI VPGLNYSMSI SYTGFIFDKL HHGVHSNYNF YEFNGKQGWI FSTDFEGGPG
SRSLMVCCDE PSYKALFHIS VRHPADMTAL SNMFHTGTTV LKEGWAVTRF RETPKMSTHL
LAICVGHFAS LSAVSETGVL VRAFSWTGME IYADFSLKIM AGAMDYMNDY FNYKFPLSKL
DVVALPQHAD RGATGKWGLI LGSYKSLIVD KDYADAKTLA NVAITVARAV VQQWFGGLAS
MEWWSEIFLS NGFAEYFATN GVNHVMPEQR EYLMNYAPFY RTSVGLWDDC RAGVSVPVIS
EDEGLFTSAV NQKASSLLHT LSNTIWEATF LKGIRTYLTN NAYRSANPEE LWNTLTEACS
EAGVPDWDGK DLDVSTFMKN WTTKVSFPIV KVSTGRNGLV TYRQESCLGD DTTWYIPIVS
VSEYNEELNW FVGKDGSSPV WQQPSPLSRV DNVGGNSFVR IYYDKITWKS MLRNMDIAND
AATQGTLLRD AWFFVSKGNY SWPQFLDLVN VIQWDDSLIK WTTGLEFFEE LYHRFRFHDS
FPRITIQQAK VGRAGQIGMQ RNPSIQRR
//
