ID A0A024G769_9STRA Unreviewed; 839 AA.
AC A0A024G769;
DT 09-JUL-2014, integrated into UniProtKB/TrEMBL.
DT 09-JUL-2014, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] {ECO:0000256|ARBA:ARBA00038890};
DE EC=1.3.1.88 {ECO:0000256|ARBA:ARBA00038890};
GN ORFNames=BN9_029550 {ECO:0000313|EMBL:CCI42171.1};
OS Albugo candida.
OC Eukaryota; Sar; Stramenopiles; Oomycota; Albuginales; Albuginaceae; Albugo.
OX NCBI_TaxID=65357 {ECO:0000313|EMBL:CCI42171.1, ECO:0000313|Proteomes:UP000053237};
RN [1] {ECO:0000313|EMBL:CCI42171.1, ECO:0000313|Proteomes:UP000053237}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ac Nc2 {ECO:0000313|EMBL:CCI42171.1,
RC ECO:0000313|Proteomes:UP000053237};
RA Gardiner A., Kemen E., Schultz-Larsen T., MacLean D., Van Oosterhout C.,
RA Jones J.D.G.;
RT "Recombination and specialization in a pathogen metapopulation.";
RL Submitted (MAY-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5,6-dihydrouridine(16) in tRNA + NAD(+) = H(+) + NADH +
CC uridine(16) in tRNA; Xref=Rhea:RHEA:53380, Rhea:RHEA-COMP:13543,
CC Rhea:RHEA-COMP:13544, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; EC=1.3.1.88;
CC Evidence={ECO:0000256|ARBA:ARBA00035900};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:53382;
CC Evidence={ECO:0000256|ARBA:ARBA00035900};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5,6-dihydrouridine(16) in tRNA + NADP(+) = H(+) + NADPH +
CC uridine(16) in tRNA; Xref=Rhea:RHEA:53376, Rhea:RHEA-COMP:13543,
CC Rhea:RHEA-COMP:13544, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; EC=1.3.1.88;
CC Evidence={ECO:0000256|ARBA:ARBA00036049};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:53378;
CC Evidence={ECO:0000256|ARBA:ARBA00036049};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5,6-dihydrouridine(17) in tRNA + NAD(+) = H(+) + NADH +
CC uridine(17) in tRNA; Xref=Rhea:RHEA:53372, Rhea:RHEA-COMP:13541,
CC Rhea:RHEA-COMP:13542, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; EC=1.3.1.88;
CC Evidence={ECO:0000256|ARBA:ARBA00035864};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:53374;
CC Evidence={ECO:0000256|ARBA:ARBA00035864};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5,6-dihydrouridine(17) in tRNA + NADP(+) = H(+) + NADPH +
CC uridine(17) in tRNA; Xref=Rhea:RHEA:53368, Rhea:RHEA-COMP:13541,
CC Rhea:RHEA-COMP:13542, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; EC=1.3.1.88;
CC Evidence={ECO:0000256|ARBA:ARBA00035813};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:53370;
CC Evidence={ECO:0000256|ARBA:ARBA00035813};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- SIMILARITY: Belongs to the Dus family. Dus1 subfamily.
CC {ECO:0000256|ARBA:ARBA00038313}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCI42171.1}.
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DR EMBL; CAIX01000030; CCI42171.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A024G769; -.
DR STRING; 65357.A0A024G769; -.
DR InParanoid; A0A024G769; -.
DR OrthoDB; 5487726at2759; -.
DR Proteomes; UP000053237; Unassembled WGS sequence.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0017150; F:tRNA dihydrouridine synthase activity; IEA:InterPro.
DR CDD; cd02801; DUS_like_FMN; 1.
DR Gene3D; 1.10.340.70; -; 1.
DR Gene3D; 2.40.50.40; -; 1.
DR Gene3D; 3.30.70.270; -; 2.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR016197; Chromo-like_dom_sf.
DR InterPro; IPR000953; Chromo/chromo_shadow_dom.
DR InterPro; IPR023780; Chromo_domain.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR035587; DUS-like_FMN-bd.
DR InterPro; IPR041588; Integrase_H2C2.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR018517; tRNA_hU_synthase_CS.
DR PANTHER; PTHR11082; TRNA-DIHYDROURIDINE SYNTHASE; 1.
DR PANTHER; PTHR11082:SF5; TRNA-DIHYDROURIDINE(16_17) SYNTHASE [NAD(P)(+)]-LIKE; 1.
DR Pfam; PF00385; Chromo; 1.
DR Pfam; PF01207; Dus; 1.
DR Pfam; PF17921; Integrase_H2C2; 1.
DR SUPFAM; SSF54160; Chromo domain-like; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF51395; FMN-linked oxidoreductases; 1.
DR PROSITE; PS50013; CHROMO_2; 1.
DR PROSITE; PS01136; UPF0034; 1.
PE 3: Inferred from homology;
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000053237};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 806..839
FT /note="Chromo"
FT /evidence="ECO:0000259|PROSITE:PS50013"
SQ SEQUENCE 839 AA; 95238 MW; 635A105974B87BE1 CRC64;
MQKLRGHEFF RSIGSPQRIV APMVDQSELA FRMLCREHGA QLCYTPMFHS RLFAESQEYR
SRMFEQHVRD RPLIVQFCGN DPETMVTAAK YVEGHCDAVD INLGCPQGIA RKGNYGAFLM
KDKARVCGIV EKLVDEVAVP VTCKIRVFPD EAETIEFTKM LEKAGCDLLV VHGRTKEMNK
GTVGQVNWEI IKKIKNALSI PVIANGGIET PEDIAECLEV TGADGVMSSE AILGNPALFD
KSLQFGPLHQ KGPCYFDLAN EYMDFVTTYP TGNLKIVRAH LFKLLFEDLT HHTDLRSALA
SANTFSEMKH LLQVLGDRKN APVKYEYTLE NIQFLSNAQG DIPVVRRTSG IGGNAWCVVT
PMRVLFDKFD FVVVYLDDIC IFSKTNEEDT THLHELFEVL RHEKPYTHRR KCSFGKESVG
FLGHNVSRNG LSVDKRKTSA IDTMAPPATR KELLSFLGLA GYNRRFICDF AKIALPLSEF
VKKEVSWEWS DDQDEAFRRL KFTFMLHHVQ GTSNVVADAR SRPSVTGPTI VTRHSQVPQV
HDCGPVCASR DVHFRRHARH MAVLCLLPRI DQDLLLDMRD FSSVDSVHQV GVLQLQQRES
HDQIQNIDFI ISSIHLSSIT KKAFMSGYTK DREFKETLNS HHDKYVLPHG LHNVKTNHAA
KRLCVPADDR LIIAILCEHH DGNTAAHPGV RRTKLKVAQW YYWPTLEKKF ESMYKAATPQ
VTAPRNGTQT TKTKLLAKEI GPFQIKKMIN NNVAKLALPR NLGKLHPSLN IELLSHFVPN
PVRFSSRPVP KAVPVIFEKK TGSELYIVEA LVQKRVYKKQ PEWLVEWHGL PEHECTWEK
//
