ID A0A024P3K7_9BACI Unreviewed; 246 AA.
AC A0A024P3K7;
DT 09-JUL-2014, integrated into UniProtKB/TrEMBL.
DT 09-JUL-2014, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE SubName: Full=Thermostable monoacylglycerol lipase {ECO:0000313|EMBL:CDQ22466.1};
GN ORFNames=BN983_00675 {ECO:0000313|EMBL:CDQ22466.1};
OS Halobacillus karajensis.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Halobacillus.
OX NCBI_TaxID=195088 {ECO:0000313|EMBL:CDQ22466.1, ECO:0000313|Proteomes:UP000028868};
RN [1] {ECO:0000313|EMBL:CDQ22466.1, ECO:0000313|Proteomes:UP000028868}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HD-03 {ECO:0000313|EMBL:CDQ22466.1,
RC ECO:0000313|Proteomes:UP000028868};
RA Urmite Genomes U.;
RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:CDQ22466.1, ECO:0000313|Proteomes:UP000028868}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HD-03 {ECO:0000313|EMBL:CDQ22466.1,
RC ECO:0000313|Proteomes:UP000028868};
RA Khelaifia S., Croce O., Lagier J.C., Raoult D.;
RT "Draft genome sequence of Halobacillus karajensis HK-03.";
RL Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDQ22466.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CCDI010000001; CDQ22466.1; -; Genomic_DNA.
DR RefSeq; WP_035505799.1; NZ_CCDJ010000003.1.
DR AlphaFoldDB; A0A024P3K7; -.
DR ESTHER; 9baci-a0a024p3k7; CarbLipBact_2.
DR Proteomes; UP000028868; Unassembled WGS sequence.
DR GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:InterPro.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR012354; Esterase_lipase.
DR InterPro; IPR022742; Hydrolase_4.
DR PANTHER; PTHR11614:SF121; CARBOXYLESTERASE; 1.
DR PANTHER; PTHR11614; PHOSPHOLIPASE-RELATED; 1.
DR Pfam; PF12146; Hydrolase_4; 1.
DR PIRSF; PIRSF017388; Esterase_lipase; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000028868}.
FT DOMAIN 20..226
FT /note="Serine aminopeptidase S33"
FT /evidence="ECO:0000259|Pfam:PF12146"
FT ACT_SITE 94
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR017388-1"
FT ACT_SITE 190
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR017388-1"
FT ACT_SITE 220
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR017388-1"
FT BINDING 26
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR017388-2"
FT BINDING 95
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR017388-2"
FT SITE 139
FT /note="Important for substrate specificity"
FT /evidence="ECO:0000256|PIRSR:PIRSR017388-3"
SQ SEQUENCE 246 AA; 27635 MW; EF2B7357D35D7EB8 CRC64;
MEKVLNGAEE IRFEGNKVGI LISHGFTGTT QSIKPLAEAY ARGGYTVYCP RLKGHGTTPE
DMETTSYQDW IASVESAYEW LLERCKKVFV VGLSMGGTLA LYMASQYKRI AGVIPINAAI
DIPALANRTD AEGRFIAAIG SDIKDPETVE LAYERTPVRS IGELVSLMDK VKQRLDEIHC
PILIFVSDED HVVPPHNSEV IFESVFSEHK ELIHLENSYH VATLDQDKEM IMERSLEFFE
MYTNTK
//
