ID   A0A024U1F2_9STRA        Unreviewed;       975 AA.
AC   A0A024U1F2;
DT   09-JUL-2014, integrated into UniProtKB/TrEMBL.
DT   09-JUL-2014, sequence version 1.
DT   24-JAN-2024, entry version 30.
DE   RecName: Full=Insulin-degrading enzyme {ECO:0008006|Google:ProtNLM};
GN   ORFNames=H310_07510 {ECO:0000313|EMBL:ETW00084.1};
OS   Aphanomyces invadans.
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Saprolegniales; Saprolegniaceae;
OC   Aphanomyces.
OX   NCBI_TaxID=157072 {ECO:0000313|EMBL:ETW00084.1};
RN   [1] {ECO:0000313|EMBL:ETW00084.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NJM9701 {ECO:0000313|EMBL:ETW00084.1};
RG   The Broad Institute Genomics Platform;
RA   Russ C., Tyler B., van West P., Dieguez-Uribeondo J., Young S.K., Zeng Q.,
RA   Gargeya S., Fitzgerald M., Abouelleil A., Alvarado L., Chapman S.B.,
RA   Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA   Imamovic A., Ireland A., Larimer J., McCowan C., Murphy C., Pearson M.,
RA   Poon T.W., Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J.,
RA   Nusbaum C., Birren B.;
RT   "The Genome Sequence of Aphanomyces invadans NJM9701.";
RL   Submitted (DEC-2013) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
CC       {ECO:0000256|ARBA:ARBA00007261, ECO:0000256|RuleBase:RU004447}.
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DR   EMBL; KI913965; ETW00084.1; -; Genomic_DNA.
DR   RefSeq; XP_008871109.1; XM_008872887.1.
DR   AlphaFoldDB; A0A024U1F2; -.
DR   STRING; 157072.A0A024U1F2; -.
DR   MEROPS; M16.008; -.
DR   EnsemblFungi; H310_07510-t26_1; H310_07510-t26_1-p1; H310_07510.
DR   EnsemblProtists; ETW00084; ETW00084; H310_07510.
DR   GeneID; 20084560; -.
DR   VEuPathDB; FungiDB:H310_07510; -.
DR   eggNOG; KOG0959; Eukaryota.
DR   OrthoDB; 129328at2759; -.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 4.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   InterPro; IPR032632; Peptidase_M16_M.
DR   PANTHER; PTHR43690:SF18; INSULIN-DEGRADING ENZYME-RELATED; 1.
DR   PANTHER; PTHR43690; NARDILYSIN; 1.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 2.
DR   Pfam; PF16187; Peptidase_M16_M; 1.
DR   SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 4.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW   Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   DOMAIN          32..164
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          191..370
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
FT   DOMAIN          376..656
FT                   /note="Peptidase M16 middle/third"
FT                   /evidence="ECO:0000259|Pfam:PF16187"
FT   DOMAIN          660..836
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
SQ   SEQUENCE   975 AA;  109567 MW;  D7EF1CB2CD1D0A87 CRC64;
     MEKSVRQKGG IDISAGDERD YRHVRLENGL CVLLVSDSEA EKSAAAMDVR VGHQSDPDNL
     LGLAHFLEHM LFMGTKKYPD ENSYSQFLSA HGGSSNAYTS GTDTNYYFDV RPPYFEEALD
     RFAQFFIAPL FTPGATEREM NAVNSENNKN LQSDPWRVDQ IVKHTSSRRH PFHKFGTGNL
     VTLGTAPTEV VRAELLAFHA KYYSARIMKL AIVGKEDLDT LEEWARSKFS EILDTGDKTF
     HYEGVPFEGP QLQRRVNVVP VKDHRSIEVS WPLPSLRSSY LQKPTSLISH LLGHEGSGSL
     LSLLKRQKWV NELSAGLMKE YDDWSLFCVE VEATEVGMAH VDEIVDSIYQ YLHLLQTDGI
     APWVFEETQS IALMNFRFRS KETPMNYATA LATRMQLYPV QHIVAGSSLL YEYDPIQVKS
     IFSLLTPDRM RLLVVGKEFE GTTTDVEPWY ETAYSDTPIE PSLIERWSNP ARSAALFCPH
     PNAFIPHNFD LVATATPGAV PILLRDDAAA RLWVKTDTTF FKPKLNICLA FHSPVIYNSP
     TSVVLTDLLV RAIKDQLTEF TYDAELAGMR YSLSFTTTAV ELYGGGYSDK LPVLFDLILK
     QIVQFDVDNE TFGRLQDKTK RSYDNFERDD PYKHALYFSS CLLEDTKWTV TEKAAAIAHV
     TRADLVHHAA ALFRELFVEA YYHGNVDAAT TTALLDNVLA TVQARPVIPS QRVKTRAIKL
     ASPAEYVYAI PELNAESVNS GIYTVFQLGP ESIHLRATNE VFAQLVREPC FNQLRTQEQL
     GYIVFSSSHR AHGIEYFRII VQSDVASPAY VERSIEAFLK LVRRDIAALS HDEFQKNLQA
     VVDALLDKPK AASEECSRFW SEISAETIAA VFQQKTVTFE KRVMTYLALL HPFLYRKFGK
     GNQGIQHQVR GINPSKKPAH GVLPVSISAK MTALHLTVQS SKNFLAASRR FGPCAATPTA
     GIVKNTTCLS KPVSR
//