ID A0A031FR21_9MICO Unreviewed; 1035 AA.
AC A0A031FR21;
DT 09-JUL-2014, integrated into UniProtKB/TrEMBL.
DT 09-JUL-2014, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=BW34_02252 {ECO:0000313|EMBL:EZP27048.1};
OS Microbacterium oleivorans.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Microbacterium.
OX NCBI_TaxID=273677 {ECO:0000313|EMBL:EZP27048.1, ECO:0000313|Proteomes:UP000024001};
RN [1] {ECO:0000313|EMBL:EZP27048.1, ECO:0000313|Proteomes:UP000024001}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RIT293 {ECO:0000313|EMBL:EZP27048.1,
RC ECO:0000313|Proteomes:UP000024001};
RA Gan H.Y., Gan H.M., Savka M.A., Hudson A.O.;
RT "Draft Genome Sequences of 13 Willow Endophytes.";
RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EZP27048.1}.
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DR EMBL; JFYO01000006; EZP27048.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A031FR21; -.
DR PATRIC; fig|273677.3.peg.2233; -.
DR eggNOG; COG0210; Bacteria.
DR eggNOG; COG2887; Bacteria.
DR Proteomes; UP000024001; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF59; DNA HELICASE; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000024001}.
FT DOMAIN 17..319
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 317..614
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 38..45
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1035 AA; 110817 MW; 37E536AC4B69F068 CRC64;
MSPVPVSVER MPAAPSRLDP AQEAVAALPA EASGVVIGAP GSGKTETLVR RVVALVEGGM
PPEQLLVLTP TRTAATALRD RLAVGVGVAT SGPLARSVAA FAYQVVRSHA VHRGEEPPQL
LTGPDEDRII GDLLAGDAED EKDGPSRWPD WLPADIRSSA GLRAEIRAFL AEMTTLGIEP
EQLATRAAAT DRPVWASLAS FAREYRSVRA RMRGAHRDAA GLVREAVGLL RDAPAGSPAL
GAVAGLRAIL VDDAQEVTLG GVELLEACRK RGVAVLAFGD PDVGSGSFRG ARPENFARLA
TTTHVLDRVH RGTAAHRDLT AQVTSRIGAA GLVSHRHPPI GAEPDESVRT FTLRSGAEEF
DLIARLLRER HVHDGVPWEQ CAVIAHDSRQ VTALEAELSA RDVPTAAVGP ARALAETLIV
RELLRAVDLA AVAADARTDD DVLDVLIGGG LDPVELRRLR AALRHTELRA GGERSARELL
ASGVRHPVEF DIIDTPEARR AARLARTLAE VGEQVQTGAT AHELLWTVWE QRGLERQLRT
LADGQGPLAD QARRDLDAVV TLFEAAKRHG EREDGTAPID YLRALLHSEV AEDRLRTGTI
TSGVQISTPA AALGLEFDTV VVAGVQDGVW PNLRLRGSLL ETSRLAEEDT DIDTADRRRA
VLHDELRLFA RAVSRARSRL VVTAVDDDDT GPSVLFELLP PASPAPRSAE HPLTLRGLVA
GHRRRLTDPR TPEPERADAA GQLALLAAAG VPGADPAHWY GLGEPTSAAP IRDLDAAPLR
ISPSRIHALE QCQLDWVIGD LGGDPGTAVA GLGTLLHAAL EHAEAGDADT LWAFVEDGWS
QLEFEAAWID EAERRRARDL VTRLAAYLRD AERDGTTLVS AEPHFEVPLP VADAAHPAIL
SGYIDRVERT RDGRVVIVDL KTGRSEGTSD RAAVDNPQLA AYQLAFERGA ITEVAGAEPG
GAKLLVLKGT RNAPYAEPRQ EPFDEDTRAA FLARVDEAVR VMRGTSFVAP FEEHCRDDHT
HGLCRIHTIG PVSSA
//
