ID A0A059B844_EUCGR Unreviewed; 507 AA.
AC A0A059B844;
DT 09-JUL-2014, integrated into UniProtKB/TrEMBL.
DT 09-JUL-2014, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=noroxomaritidine synthase {ECO:0000256|ARBA:ARBA00039071};
DE EC=1.14.19.50 {ECO:0000256|ARBA:ARBA00039071};
GN ORFNames=EUGRSUZ_H04870 {ECO:0000313|EMBL:KCW62209.1};
OS Eucalyptus grandis (Flooded gum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Myrtales; Myrtaceae; Myrtoideae; Eucalypteae; Eucalyptus.
OX NCBI_TaxID=71139 {ECO:0000313|EMBL:KCW62209.1};
RN [1] {ECO:0000313|EMBL:KCW62209.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf extractions {ECO:0000313|EMBL:KCW62209.1};
RA Schmutz J., Hayes R., Myburg A., Tuskan G., Grattapaglia D., Rokhsar D.S.;
RT "The genome of Eucalyptus grandis.";
RL Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4'-O-methylnorbelladine + O2 + reduced [NADPH--hemoprotein
CC reductase] = (10bR,4aS)-noroxomaritidine + H(+) + 2 H2O + oxidized
CC [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:51260, Rhea:RHEA-
CC COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:57618,
CC ChEBI:CHEBI:58210, ChEBI:CHEBI:133993, ChEBI:CHEBI:133995;
CC EC=1.14.19.50; Evidence={ECO:0000256|ARBA:ARBA00036965};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4'-O-methylnorbelladine + O2 + reduced [NADPH--hemoprotein
CC reductase] = (10bS,4aR)-noroxomaritidine + H(+) + 2 H2O + oxidized
CC [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:51264, Rhea:RHEA-
CC COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:57618,
CC ChEBI:CHEBI:58210, ChEBI:CHEBI:133993, ChEBI:CHEBI:133996;
CC EC=1.14.19.50; Evidence={ECO:0000256|ARBA:ARBA00035970};
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000256|ARBA:ARBA00001971};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004167}; Single-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004167}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family.
CC {ECO:0000256|ARBA:ARBA00010617, ECO:0000256|RuleBase:RU000461}.
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DR EMBL; KK198760; KCW62209.1; -; Genomic_DNA.
DR RefSeq; XP_010027344.1; XM_010029042.1.
DR AlphaFoldDB; A0A059B844; -.
DR EnsemblPlants; KCW62209; KCW62209; EUGRSUZ_H04870.
DR Gramene; KCW62209; KCW62209; EUGRSUZ_H04870.
DR eggNOG; KOG0157; Eukaryota.
DR InParanoid; A0A059B844; -.
DR OMA; NTRILWK; -.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR CDD; cd11064; CYP86A; 1.
DR Gene3D; 1.10.630.10; Cytochrome P450; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR PANTHER; PTHR24296; CYTOCHROME P450; 1.
DR PANTHER; PTHR24296:SF1; CYTOCHROME P450, FAMILY 96, SUBFAMILY A, POLYPEPTIDE 10; 1.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; Cytochrome P450; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 3: Inferred from homology;
KW Heme {ECO:0000256|RuleBase:RU000461};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU000461};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU000461};
KW Monooxygenase {ECO:0000256|RuleBase:RU000461};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU000461}.
SQ SEQUENCE 507 AA; 58025 MW; 11400447F50D5D3B CRC64;
MAVIGFLEIF LAGICFAVLC LLSKSDDGFP RNWPLVGMLP ALFLNLSRVH DWATGLVERS
HGNFFFRSLW FSSMDMLVTA DPANFHYIMS ANFGNFPKGP EFKRVFDILG DGIINSDSNL
WSTQRRVAQV FMKDQRFHRF LLSTTRDKAE NGLLRVLDEL SQRGVTVDLQ DLFHRFTFDS
ACRFVTGFDP GSLSVEFPEV PISKALGDAE EVIFYRHLYP EWFGTLQRLL GIGVEEKLRR
AWKDIDQILG NYIAMKRVEL REGTNKVKGD ENGADLLTLY MVENETLGAL ECNDKFLRDM
IMNFMSAGIE TTGSTLTWFF WALSMNPSVD MRIREEIRSV IPKEEQNLMV FNISEHMHKM
VYLHGAICES LRLYPPIPLL PKCPTNPDVL PSGHKVDPGA KIVVHMYAMG RMKSIWGEDC
LEFRPERWIL ERGGIRHQPS YKFVAFNAGP RTCLGKDVAF TQIKMVAATV IHNYEVHVVE
DHPVRPSNSI ILHMKHGLKV KVTRRRL
//
