ID   A0A059BK70_EUCGR        Unreviewed;       553 AA.
AC   A0A059BK70;
DT   09-JUL-2014, integrated into UniProtKB/TrEMBL.
DT   09-JUL-2014, sequence version 1.
DT   27-MAR-2024, entry version 34.
DE   RecName: Full=carotenoid 9,10-dioxygenase {ECO:0000256|ARBA:ARBA00039084};
DE            EC=1.14.99.n4 {ECO:0000256|ARBA:ARBA00039084};
GN   ORFNames=EUGRSUZ_G03374 {ECO:0000313|EMBL:KCW66100.1};
OS   Eucalyptus grandis (Flooded gum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Myrtales; Myrtaceae; Myrtoideae; Eucalypteae; Eucalyptus.
OX   NCBI_TaxID=71139 {ECO:0000313|EMBL:KCW66100.1};
RN   [1] {ECO:0000313|EMBL:KCW66100.1}
RP   NUCLEOTIDE SEQUENCE.
RC   TISSUE=Leaf extractions {ECO:0000313|EMBL:KCW66100.1};
RA   Schmutz J., Hayes R., Myburg A., Tuskan G., Grattapaglia D., Rokhsar D.S.;
RT   "The genome of Eucalyptus grandis.";
RL   Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-zeaxanthin + 2 O2 = 2 (3R)-hydroxy-beta-ionone +
CC         4,9-dimethyldodeca-2,4,6,8,10-pentaenedial; Xref=Rhea:RHEA:26393,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:27547, ChEBI:CHEBI:53171,
CC         ChEBI:CHEBI:53173; EC=1.14.99.n4;
CC         Evidence={ECO:0000256|ARBA:ARBA00036367};
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000256|PIRSR:PIRSR604294-1};
CC       Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR604294-1};
CC   -!- SIMILARITY: Belongs to the carotenoid oxygenase family.
CC       {ECO:0000256|ARBA:ARBA00006787}.
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DR   EMBL; KK198759; KCW66100.1; -; Genomic_DNA.
DR   RefSeq; XP_010067867.1; XM_010069565.2.
DR   AlphaFoldDB; A0A059BK70; -.
DR   EnsemblPlants; KCW66100; KCW66100; EUGRSUZ_G03374.
DR   GeneID; 104454652; -.
DR   Gramene; KCW66100; KCW66100; EUGRSUZ_G03374.
DR   KEGG; egr:104454652; -.
DR   eggNOG; KOG1285; Eukaryota.
DR   OrthoDB; 318119at2759; -.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:InterPro.
DR   InterPro; IPR004294; Carotenoid_Oase.
DR   PANTHER; PTHR10543; BETA-CAROTENE DIOXYGENASE; 1.
DR   PANTHER; PTHR10543:SF89; CAROTENOID 9,10(9',10')-CLEAVAGE DIOXYGENASE 1; 1.
DR   Pfam; PF03055; RPE65; 1.
PE   3: Inferred from homology;
KW   Dioxygenase {ECO:0000256|ARBA:ARBA00022964};
KW   Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR604294-1};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR604294-1};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT   BINDING         234
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT   BINDING         282
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT   BINDING         348
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT   BINDING         538
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
SQ   SEQUENCE   553 AA;  62388 MW;  789A54552839928C CRC64;
     MAEREEKGKY DEMMMKKGSD GGIAEVNPTP KKGVTSKVVD YIEKLIVKFM YDSSRPHQYL
     AGNFAPVADE TPPVTDLPVV GHLPDCLNGE FVRVGPNPKF APVAGYHWFD GDGMVHGMRI
     KNGKATYVSR YVRTSKLKQE EYYGGAKFMK IGDLKGLFGL LMVNMQMLRA KLKILDVSYG
     TGTGNTALVY HHGKLLALSE ADKPYVLKVL EDGDLQTLGM LDYDKRLSHS FTAHPKVDPF
     TGEMFTFGYS HTPPYITYRV ISKDGFMHDP VPITISDPIM MHDFAITENY AIFMDLPLYF
     RPKEMVKDNK LIFTFDKTKK SRFGVLPRYA KNELQIRWFE LPNCFIFHNA NAWEEEDEVV
     LVTCRLENLD LDMVSGTVKE HLENFGNELY EMRFNMKTGL ASQKKLSAPS VDFPRINECY
     TGRKQRYVYG TILDSIAKVT GIVKFDLHAE PEEGKKKIEV GGNVAGVFDL GPGRFGSEAV
     FVPREPGITS EEDDGYLIFF VHDESTGKSA VNVIDAKNMS SDPVAVVELP HRVPYGFHAF
     FVTEEQLQEL AKL
//