ID A0A059CBW6_EUCGR Unreviewed; 271 AA.
AC A0A059CBW6;
DT 09-JUL-2014, integrated into UniProtKB/TrEMBL.
DT 09-JUL-2014, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=3-hydroxyisobutyrate dehydrogenase {ECO:0000256|ARBA:ARBA00012991};
DE EC=1.1.1.31 {ECO:0000256|ARBA:ARBA00012991};
DE Flags: Fragment;
GN ORFNames=EUGRSUZ_D001292 {ECO:0000313|EMBL:KCW75749.1};
OS Eucalyptus grandis (Flooded gum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Myrtales; Myrtaceae; Myrtoideae; Eucalypteae; Eucalyptus.
OX NCBI_TaxID=71139 {ECO:0000313|EMBL:KCW75749.1};
RN [1] {ECO:0000313|EMBL:KCW75749.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf extractions {ECO:0000313|EMBL:KCW75749.1};
RA Schmutz J., Hayes R., Myburg A., Tuskan G., Grattapaglia D., Rokhsar D.S.;
RT "The genome of Eucalyptus grandis.";
RL Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-
CC oxopropanoate + H(+) + NADH; Xref=Rhea:RHEA:17681, ChEBI:CHEBI:11805,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57700,
CC ChEBI:CHEBI:57945; EC=1.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00000062};
CC -!- PATHWAY: Amino-acid degradation; L-valine degradation.
CC {ECO:0000256|ARBA:ARBA00005109}.
CC -!- SIMILARITY: Belongs to the HIBADH-related family. 3-hydroxyisobutyrate
CC dehydrogenase subfamily. {ECO:0000256|ARBA:ARBA00006013}.
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DR EMBL; KK198756; KCW75749.1; -; Genomic_DNA.
DR EMBL; KK198756; KCW75750.1; -; Genomic_DNA.
DR STRING; 71139.A0A059CBW6; -.
DR EnsemblPlants; KCW75749; KCW75749; EUGRSUZ_D001292.
DR EnsemblPlants; KCW75750; KCW75750; EUGRSUZ_D001292.
DR Gramene; KCW75749; KCW75749; EUGRSUZ_D001292.
DR Gramene; KCW75750; KCW75750; EUGRSUZ_D001292.
DR OMA; NNMMLFI; -.
DR GO; GO:0008442; F:3-hydroxyisobutyrate dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0006574; P:valine catabolic process; IBA:GO_Central.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR002204; 3-OH-isobutyrate_DH-rel_CS.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR013328; 6PGD_dom2.
DR InterPro; IPR006115; 6PGDH_NADP-bd.
DR InterPro; IPR029154; HIBADH-like_NADP-bd.
DR InterPro; IPR015815; HIBADH-related.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR22981:SF7; 3-HYDROXYISOBUTYRATE DEHYDROGENASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR22981; 3-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATED; 1.
DR Pfam; PF14833; NAD_binding_11; 1.
DR Pfam; PF03446; NAD_binding_2; 1.
DR PIRSF; PIRSF000103; HIBADH; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS00895; 3_HYDROXYISOBUT_DH; 1.
PE 3: Inferred from homology;
KW Branched-chain amino acid catabolism {ECO:0000256|ARBA:ARBA00022456};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 47..218
FT /note="6-phosphogluconate dehydrogenase NADP-binding"
FT /evidence="ECO:0000259|Pfam:PF03446"
FT DOMAIN 221..271
FT /note="3-hydroxyisobutyrate dehydrogenase-like NAD-binding"
FT /evidence="ECO:0000259|Pfam:PF14833"
FT REGION 12..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 227
FT /evidence="ECO:0000256|PIRSR:PIRSR000103-1"
FT NON_TER 271
FT /evidence="ECO:0000313|EMBL:KCW75749.1"
SQ SEQUENCE 271 AA; 27807 MW; 5EFD204F095E9712 CRC64;
MASRAARALL SLPKRKPNVS PSPLLRPPAP ARGLCSSPDE GPANFGNVGF IGLGNMGSRM
AKNLVKAGYN VAVHDINGDA MKIFAGFGVP TKKTPFEVAE ESDVIVTMLP SSSHVFDVYT
GPNGLLHGGN LLRPWLLIDS STIDPQTSRK ISALVSNCTL NGKRDGGGVP MMLDAPVSGG
VLAAEAGSLT FMVGGSEEAY LTAKPLLLAM GKSTIYCGGA GNGSAAKICN NLAMAVSMLG
VSEALALGQS LGIAASVLTR VFNSSSARCW S
//
