ID A0A059CWA6_EUCGR Unreviewed; 763 AA.
AC A0A059CWA6;
DT 09-JUL-2014, integrated into UniProtKB/TrEMBL.
DT 09-JUL-2014, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
GN ORFNames=EUGRSUZ_C03662 {ECO:0000313|EMBL:KCW82245.1};
OS Eucalyptus grandis (Flooded gum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Myrtales; Myrtaceae; Myrtoideae; Eucalypteae; Eucalyptus.
OX NCBI_TaxID=71139 {ECO:0000313|EMBL:KCW82245.1};
RN [1] {ECO:0000313|EMBL:KCW82245.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf extractions {ECO:0000313|EMBL:KCW82245.1};
RA Schmutz J., Hayes R., Myburg A., Tuskan G., Grattapaglia D., Rokhsar D.S.;
RT "The genome of Eucalyptus grandis.";
RL Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
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DR EMBL; KK198755; KCW82245.1; -; Genomic_DNA.
DR RefSeq; XP_018727062.1; XM_018871517.1.
DR AlphaFoldDB; A0A059CWA6; -.
DR EnsemblPlants; KCW82245; KCW82245; EUGRSUZ_C03662.
DR Gramene; KCW82245; KCW82245; EUGRSUZ_C03662.
DR eggNOG; KOG0496; Eukaryota.
DR InParanoid; A0A059CWA6; -.
DR OMA; CANITKS; -.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF63; BETA-GALACTOSIDASE 12; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 2.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..31
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 32..763
FT /note="Beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5001569656"
FT DOMAIN 40..342
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 367..423
FT /note="Beta-galactosidase beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF17834"
FT DOMAIN 474..541
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT DOMAIN 624..709
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
SQ SEQUENCE 763 AA; 84532 MW; 7CB37FC55AD68E57 CRC64;
MGSKFHAFFP VIGARLVLAL VLISWSRSVS CSVSYDRKAI VIDGQRRILI SGSIHYPRST
PEMWPDLLQK AKAGGLDVIQ TYVFWNGHEP SRGKYYFEDR FDLVRFIKLV QHAGLYVHLR
IGPYVCAEWN FGGFPVWLKY VPGIAFRTDN QPFKDAMQGF TQKIVDMMKA EKLFQPQGGP
IILSQIENEF ELVEWEIGEP GKAYTKWAAD MAVGLGTGVP WVMCKQADAP DPVINTCNGF
YCENFEPNSN DKPKMWTEVW TGWFTGFGGA VLHRPAEDLA FAVTRFIQNH GSFFNYYMYH
GGTNFGRTTG GPFIATSYDF DAPIDEFGSL REPKWSHLRG LHLAIKQSES ALVAADPAVS
SLGSKQEAHV FESGSGACAA FLANYDTQDS VKVTFRNRQY ELSPWSISIL PDCQTAVYNT
ATVGYKSSPI QMVPIKSAFP WQSYREETFC FGEYGTINEG LPEQISVTRD VSDYLWYAIN
FTISQDEAFL TNGQYPVLNV SSAGHALHVF VNGQQAGSVY GGQENPKLTY SDKVKLTAGI
NKVSVLSVAV GLPNIGLHYE TWNAGVLGPI TLEGMNSGKW DLTKWKWSSK VGLKGEDLSL
HTSSGSSSVE WDGGSTLAQN QPLIWYKTTF HAPVGNTPLA LDMNGMGKGQ IWINGRSIGR
HWPAYTAHGT CGTCNYAGTF DLSKCLSNCG EASQRWYHIP RSWLNPTGNL LVVFEEWGGD
PTQVSLVQRM GGSQGAGTSH GPHMAFLVFM LLIAGIKQLP LFR
//
