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Database: UniProt
Entry: A0A059XVZ5_9MOLU
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ID   A0A059XVZ5_9MOLU        Unreviewed;       623 AA.
AC   A0A059XVZ5;
DT   03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT   03-SEP-2014, sequence version 1.
DT   08-MAY-2019, entry version 30.
DE   RecName: Full=DNA primase {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00993443};
DE            EC=2.7.7.- {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00993444};
GN   Name=dnaG {ECO:0000256|HAMAP-Rule:MF_00974,
GN   ECO:0000313|EMBL:AIA29422.1};
GN   ORFNames=MCAL160E_0658 {ECO:0000313|EMBL:BBG42764.1}, MCFN_01390
GN   {ECO:0000313|EMBL:AIA29422.1};
OS   Mycoplasma californicum.
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=2113 {ECO:0000313|EMBL:AIA29422.1, ECO:0000313|Proteomes:UP000027088};
RN   [1] {ECO:0000313|EMBL:AIA29422.1, ECO:0000313|Proteomes:UP000027088}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ST-6 {ECO:0000313|EMBL:AIA29422.1,
RC   ECO:0000313|Proteomes:UP000027088};
RX   PubMed=24994797;
RA   Calcutt M.J., Foecking M.F., Fox L.K.;
RT   "Complete Genome Sequence of the Bovine Mastitis Pathogen Mycoplasma
RT   californicum Strain ST-6T (ATCC 33461T).";
RL   Genome Announc. 2:e00648-14(2014).
RN   [2] {ECO:0000313|Proteomes:UP000270760}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HAZ160_1erythromycin {ECO:0000313|Proteomes:UP000270760};
RG   Mycoplasma californicum genome sequencing consortium;
RA   Hata E., Chikayama Y.;
RL   Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|Proteomes:UP000270760}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HAZ160_1erythromycin {ECO:0000313|Proteomes:UP000270760};
RA   Hata E.;
RT   "Emergence of Mycoplasma californicum resistance against lincosamides
RT   and/or 15- and 16-membered macrolides: Analysis of resistance
RT   mechanisms and development of rapid identification method for
RT   mutations involved in resistance.";
RL   Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
RN   [4] {ECO:0000313|EMBL:BBG42764.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=HAZ160_1erythromycin {ECO:0000313|EMBL:BBG42764.1};
RX   PubMed=30642602; DOI=10.1016/j.vetmic.2018.12.017;
RA   Hata E., Nagai K., Murakami K.;
RT   "Mutations associated with change of susceptibility to lincosamides
RT   and/or macrolides in field and laboratory-derived Mycoplasma
RT   californicum strains in Japan, and development of a rapid detection
RT   method for these mutations.";
RL   Vet. Microbiol. 229:81-89(2019).
CC   -!- FUNCTION: RNA polymerase that catalyzes the synthesis of short RNA
CC       molecules used as primers for DNA polymerase during DNA
CC       replication. {ECO:0000256|HAMAP-Rule:MF_00974,
CC       ECO:0000256|SAAS:SAAS00709340}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|SAAS:SAAS00709317};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00974};
CC       Note=Binds 1 zinc ion per monomer. {ECO:0000256|HAMAP-
CC       Rule:MF_00974};
CC   -!- SUBUNIT: Monomer. Interacts with DnaB. {ECO:0000256|HAMAP-
CC       Rule:MF_00974}.
CC   -!- DOMAIN: Contains an N-terminal zinc-binding domain, a central core
CC       domain that contains the primase activity, and a C-terminal DnaB-
CC       binding domain. {ECO:0000256|HAMAP-Rule:MF_00974}.
CC   -!- SIMILARITY: Belongs to the DnaG primase family.
CC       {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00709351}.
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DR   EMBL; CP007521; AIA29422.1; -; Genomic_DNA.
DR   EMBL; AP018943; BBG42764.1; -; Genomic_DNA.
DR   RefSeq; WP_051604554.1; NZ_CP007521.1.
DR   STRING; 2113.MCFN_01390; -.
DR   EnsemblBacteria; AIA29422; AIA29422; MCFN_01390.
DR   KEGG; mcr:MCFN_01390; -.
DR   KO; K02316; -.
DR   OrthoDB; 1071997at2; -.
DR   Proteomes; UP000027088; Chromosome.
DR   Proteomes; UP000270760; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   CDD; cd03364; TOPRIM_DnaG_primases; 1.
DR   Gene3D; 3.90.580.10; -; 1.
DR   Gene3D; 3.90.980.10; -; 1.
DR   HAMAP; MF_00974; DNA_primase_DnaG; 1.
DR   InterPro; IPR013264; DNA_primase_core_N.
DR   InterPro; IPR037068; DNA_primase_core_N_sf.
DR   InterPro; IPR006295; DNA_primase_DnaG.
DR   InterPro; IPR036977; DNA_primase_Znf_CHC2.
DR   InterPro; IPR030846; DnaG_bac.
DR   InterPro; IPR034151; TOPRIM_DnaG_bac.
DR   InterPro; IPR006171; TOPRIM_domain.
DR   InterPro; IPR002694; Znf_CHC2.
DR   Pfam; PF08275; Toprim_N; 1.
DR   Pfam; PF01807; zf-CHC2; 1.
DR   SMART; SM00493; TOPRIM; 1.
DR   SMART; SM00400; ZnF_CHCC; 1.
DR   TIGRFAMs; TIGR01391; dnaG; 1.
DR   PROSITE; PS50880; TOPRIM; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000027088,
KW   ECO:0000313|Proteomes:UP000270760};
KW   DNA replication {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00993445};
KW   DNA-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709369};
KW   DNA-directed RNA polymerase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709327};
KW   Magnesium {ECO:0000256|SAAS:SAAS00709345};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709338};
KW   Nucleotidyltransferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709339};
KW   Primosome {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709304};
KW   Reference proteome {ECO:0000313|Proteomes:UP000027088};
KW   Transcription {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709341};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00993442};
KW   Zinc {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00709300};
KW   Zinc-finger {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709301}.
FT   DOMAIN      256    335       Toprim. {ECO:0000259|PROSITE:PS50880}.
FT   ZN_FING      40     64       CHC2-type. {ECO:0000256|HAMAP-Rule:
FT                                MF_00974}.
SQ   SEQUENCE   623 AA;  72793 MW;  58AF96C921D1CCFC CRC64;
     MNTDNLQSLK DEILSQLDIV DEISKHVTLV KKGQSYVCLC PFHDDSNPSL TINRNKQIFK
     CFVCQVGGDV IKFVSKFKKI SWYDSLKYLA NELNLDYDNT LFMTEPSRYS AFDLEVTELN
     EEVNSFFKIN FKKLKNTTLN NFFLSRDLNT DLLNNFDIGY CEARVFTEFF GSKIHEKPLI
     FAKAGLIHVD TLAPTFDNRV TFAIRDDQGK VVGFSARSLD KDAKPKYINS SESTLFQKNA
     LLYNYWRAKD ATHEQKRLII VEGFFDVIAL YKIGKDNVVA LMGTSLTNNH LKFLHNKDII
     LFLDGDQAGQ SASLKNAKFL LSKNINVHIV KNQTRLDPDE IIKKYGIEYI QTMLDKAQLA
     TDFIYDYFKH QYNLQPDSSN NLDSISNFVD SFFEYINVCN QNIRNYYIDR IKNEFSFDPA
     ININLSKNTQ KHSYNADYTN EFTQSFYTNQ AFVEDNYIYQ HELPQKPYLA SEQKTESLSW
     IDRLFYAILE HPSLRELFIQ RANSNEGKLY LKVFKDYKSE VYDLITQPDC DLEYVKKILA
     PNDQKRQEFD SYTQDFYRNT EQNYNNLLEN FNFAYRNAQN ESFRRYAMET ATPEFSDRAN
     KLPAVFSDHA INSKNSFNSK NKK
//
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