ID A0A060HTP4_9ARCH Unreviewed; 363 AA.
AC A0A060HTP4;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE SubName: Full=Fused DNA-binding transcriptional dual regulator O6-methylguanine-DNA methyltransferase {ECO:0000313|EMBL:AIC16462.1};
DE EC=2.1.1.63 {ECO:0000313|EMBL:AIC16462.1};
GN Name=ada {ECO:0000313|EMBL:AIC16462.1};
GN ORFNames=NVIE_022010 {ECO:0000313|EMBL:AIC16462.1};
OS Nitrososphaera viennensis EN76.
OC Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
OC Nitrososphaeraceae; Nitrososphaera.
OX NCBI_TaxID=926571 {ECO:0000313|EMBL:AIC16462.1, ECO:0000313|Proteomes:UP000027093};
RN [1] {ECO:0000313|EMBL:AIC16462.1, ECO:0000313|Proteomes:UP000027093}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EN76 {ECO:0000313|EMBL:AIC16462.1};
RX PubMed=24907263; DOI=10.1099/ijs.0.063172-0;
RA Stieglmeier M., Klingl A., Alves R.J., Rittmann S.K., Melcher M.,
RA Leisch N., Schleper C.;
RT "Nitrososphaera viennensis gen. nov., sp. nov., an aerobic and mesophilic,
RT ammonia-oxidizing archaeon from soil and a member of the archaeal phylum
RT Thaumarchaeota.";
RL Int. J. Syst. Evol. Microbiol. 64:2738-2752(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a
CC thymidine in DNA + S-methyl-L-cysteinyl-[protein];
CC Xref=Rhea:RHEA:53428, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:10132,
CC Rhea:RHEA-COMP:13555, Rhea:RHEA-COMP:13556, ChEBI:CHEBI:29950,
CC ChEBI:CHEBI:82612, ChEBI:CHEBI:137386, ChEBI:CHEBI:137387;
CC EC=2.1.1.63; Evidence={ECO:0000256|ARBA:ARBA00001286};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein]
CC = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein];
CC Xref=Rhea:RHEA:24000, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:10132,
CC Rhea:RHEA-COMP:11367, Rhea:RHEA-COMP:11368, ChEBI:CHEBI:29950,
CC ChEBI:CHEBI:82612, ChEBI:CHEBI:85445, ChEBI:CHEBI:85448; EC=2.1.1.63;
CC Evidence={ECO:0000256|ARBA:ARBA00001596};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
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DR EMBL; CP007536; AIC16462.1; -; Genomic_DNA.
DR RefSeq; WP_075055215.1; NZ_CP007536.1.
DR AlphaFoldDB; A0A060HTP4; -.
DR STRING; 926571.NVIE_022010; -.
DR GeneID; 74947443; -.
DR KEGG; nvn:NVIE_022010; -.
DR HOGENOM; CLU_000445_52_0_2; -.
DR OrthoDB; 372118at2157; -.
DR Proteomes; UP000027093; Chromosome.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0003908; F:methylated-DNA-[protein]-cysteine S-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd06445; ATase; 1.
DR Gene3D; 3.40.10.10; DNA Methylphosphotriester Repair Domain; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR Gene3D; 3.30.160.70; Methylated DNA-protein cysteine methyltransferase domain; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR035451; Ada-like_dom_sf.
DR InterPro; IPR004026; Ada_DNA_repair_Zn-bd.
DR InterPro; IPR016221; Bifunct_regulatory_prot_Ada.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR001497; MethylDNA_cys_MeTrfase_AS.
DR InterPro; IPR014048; MethylDNA_cys_MeTrfase_DNA-bd.
DR InterPro; IPR036217; MethylDNA_cys_MeTrfase_DNAb.
DR InterPro; IPR036631; MGMT_N_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR NCBIfam; TIGR00589; ogt; 1.
DR PANTHER; PTHR10815:SF14; BIFUNCTIONAL TRANSCRIPTIONAL ACTIVATOR_DNA REPAIR ENZYME ADA; 1.
DR PANTHER; PTHR10815; METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE; 1.
DR Pfam; PF02805; Ada_Zn_binding; 1.
DR Pfam; PF01035; DNA_binding_1; 1.
DR Pfam; PF12833; HTH_18; 1.
DR PIRSF; PIRSF000409; Ada; 1.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF57884; Ada DNA repair protein, N-terminal domain (N-Ada 10); 1.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR SUPFAM; SSF53155; Methylated DNA-protein cysteine methyltransferase domain; 1.
DR SUPFAM; SSF46767; Methylated DNA-protein cysteine methyltransferase, C-terminal domain; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
DR PROSITE; PS00374; MGMT; 1.
PE 4: Predicted;
KW Activator {ECO:0000256|ARBA:ARBA00023159};
KW DNA damage {ECO:0000256|ARBA:ARBA00023204};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000313|EMBL:AIC16462.1};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:AIC16462.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000027093};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:AIC16462.1}.
FT DOMAIN 93..189
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000259|PROSITE:PS01124"
SQ SEQUENCE 363 AA; 39525 MW; 85F27423A3D32D1F CRC64;
MTPKNAQLAA ATESDPRWAS VVARDHRADG AFYFSVKTTG VYCRPSCAAR RARPENVSFF
TTRKDAEKAG LRPCKRCKPD QASLAEQHAA KVTQACRLIE ESENAPSLEE LAKHAGTSAY
HFHRVFKAVT GLTPREYALA HRAKRVRNEL GQSETVTEAI YGAGYRSNRR FYDTSNDVLG
MTPSSYRAGG ARTEIRFAVG ECSLGSILVA QSERGVCAIL LGDDPGELAR DLQDRFPRAN
LIGGDAKFEQ LVSKVVGFVE APALGLDLPL DVRGTAFQQR VWQALREIPA GSTASYADIA
NRIGSPKSVR VVAQACAANA LAVAIPCHRV VKNDGALSGY RWGVERKRAL LNMEAPHAHE
RTD
//
