ID A0A060LWB4_9BACI Unreviewed; 779 AA.
AC A0A060LWB4;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 49.
DE RecName: Full=Ribonuclease R {ECO:0000256|HAMAP-Rule:MF_01895};
DE Short=RNase R {ECO:0000256|HAMAP-Rule:MF_01895};
DE EC=3.1.13.1 {ECO:0000256|HAMAP-Rule:MF_01895};
GN Name=rnr {ECO:0000256|HAMAP-Rule:MF_01895};
GN ORFNames=BleG1_2987 {ECO:0000313|EMBL:AIC95551.1};
OS Shouchella lehensis G1.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Shouchella.
OX NCBI_TaxID=1246626 {ECO:0000313|EMBL:AIC95551.1, ECO:0000313|Proteomes:UP000027142};
RN [1] {ECO:0000313|EMBL:AIC95551.1, ECO:0000313|Proteomes:UP000027142}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=G1 {ECO:0000313|EMBL:AIC95551.1,
RC ECO:0000313|Proteomes:UP000027142};
RX PubMed=24811681; DOI=10.1016/j.gene.2014.05.012;
RA Noor Y.M., Samsulrizal N.H., Jema'on N.A., Low K.O., Ramli A.N.,
RA Alias N.I., Damis S.I., Fuzi S.F., Isa M.N., Murad A.M., Raih M.F.,
RA Bakar F.D., Najimudin N., Mahadi N.M., Illias R.M.;
RT "A comparative genomic analysis of the alkalitolerant soil bacterium
RT Bacillus lehensis G1.";
RL Gene 545:253-261(2014).
CC -!- FUNCTION: 3'-5' exoribonuclease that releases 5'-nucleoside
CC monophosphates and is involved in maturation of structured RNAs.
CC {ECO:0000256|HAMAP-Rule:MF_01895}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC nucleoside 5'-phosphates.; EC=3.1.13.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001849, ECO:0000256|HAMAP-
CC Rule:MF_01895};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|HAMAP-Rule:MF_01895}.
CC -!- SIMILARITY: Belongs to the RNR ribonuclease family. RNase R subfamily.
CC {ECO:0000256|HAMAP-Rule:MF_01895}.
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DR EMBL; CP003923; AIC95551.1; -; Genomic_DNA.
DR RefSeq; WP_038482541.1; NZ_CP003923.1.
DR AlphaFoldDB; A0A060LWB4; -.
DR STRING; 1246626.BleG1_2987; -.
DR KEGG; ble:BleG1_2987; -.
DR PATRIC; fig|1246626.3.peg.2977; -.
DR eggNOG; COG0557; Bacteria.
DR HOGENOM; CLU_002333_7_0_9; -.
DR OrthoDB; 9764149at2; -.
DR Proteomes; UP000027142; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008859; F:exoribonuclease II activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016070; P:RNA metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd04471; S1_RNase_R; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 2.
DR HAMAP; MF_01895; RNase_R; 1.
DR InterPro; IPR011129; CSD.
DR InterPro; IPR040476; CSD2.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR013223; RNase_B_OB_dom.
DR InterPro; IPR001900; RNase_II/R.
DR InterPro; IPR022966; RNase_II/R_CS.
DR InterPro; IPR004476; RNase_II/RNase_R.
DR InterPro; IPR011805; RNase_R.
DR InterPro; IPR003029; S1_domain.
DR NCBIfam; TIGR00358; 3_prime_RNase; 1.
DR NCBIfam; TIGR02063; RNase_R; 1.
DR PANTHER; PTHR23355:SF9; DIS3-LIKE EXONUCLEASE 2; 1.
DR PANTHER; PTHR23355; RIBONUCLEASE; 1.
DR Pfam; PF17876; CSD2; 1.
DR Pfam; PF08206; OB_RNB; 1.
DR Pfam; PF00773; RNB; 1.
DR Pfam; PF00575; S1; 1.
DR SMART; SM00357; CSP; 1.
DR SMART; SM00955; RNB; 1.
DR SMART; SM00316; S1; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 4.
DR PROSITE; PS01175; RIBONUCLEASE_II; 1.
DR PROSITE; PS50126; S1; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01895};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839, ECO:0000256|HAMAP-
KW Rule:MF_01895};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_01895};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|HAMAP-Rule:MF_01895};
KW Reference proteome {ECO:0000313|Proteomes:UP000027142};
KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_01895}.
FT DOMAIN 627..707
FT /note="S1 motif"
FT /evidence="ECO:0000259|PROSITE:PS50126"
FT REGION 711..779
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 711..768
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 779 AA; 88582 MW; F899005538661E8C CRC64;
MSDQINKKEL IQYFKEEATK PVSVEELEER FQITSSEAFK ELITLLNELE GEGEVIRTRT
NRYGIPEKLN LIRGRVQGHS KGFAFIIPDD GDEDVYVPQG SLSGAMNGDI VFVRIDERAN
GSRAEGKVIR IIERKVTEIV GEYKEFKSYA LVVADDKRIS ADFLVIKGKE KGAVDGHKVI
ATITQYPEGR FGGEVEIKEV LGHKNDPGMD ILSIIYKYSI PQSFSDDTLE QAENVPDAID
SNDLKGRRDL RDEQIVTIDG ADAKDLDDAV QVKKLPNGHY LLGVHIADVS HYVKEGSPID
KEASERATSV YLVDRVIPMI PHRLSNGICS LNPQVDRLTL SCEMEIDQNG GVVKHDIFQS
VINTTERMTY SDVNDILVNK DESLRERYST LVPMFEEMEK LAQVLRDKRF GRGAIDFDFK
EAKVLVNSEG TPEDVVLRER SVAEKLIEEF MLAANETVAE HFHWMKLPFV YRIHEDPDAE
KLNSFLEFIT GFGYVVRGTA NTIHPRALQS LLKEIQGEPE ETIISRIMLR SMKQAKYDVH
SVGHFGLSAE FYTHFTSPIR RYPDLLVHRL IRTYLIQGKT DAKTTGHWQE KLPGLTAHAS
DMERRAVDAE RDTDNVKKAQ YMEDKIGEVY TGMISGVTNF GLFVELENTV EGLVHVSYLT
DDYYRYDQKH YAMIGERSGN VFRIGDELEI RVVSVNTEEA SVDFEIVGMK KRKPQRGKDR
PKVIDGGKRV NKQQKKDRPR LTGDPKKDGQ PKSGKRKKKP FYENAPSVKR KKGRRKKRS
//
