ID A0A060LXU0_9BACI Unreviewed; 928 AA.
AC A0A060LXU0;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=3'-5' exonuclease DinG {ECO:0000256|HAMAP-Rule:MF_02206, ECO:0000256|RuleBase:RU364106};
DE EC=3.1.-.- {ECO:0000256|HAMAP-Rule:MF_02206, ECO:0000256|RuleBase:RU364106};
GN Name=dinG {ECO:0000256|HAMAP-Rule:MF_02206,
GN ECO:0000256|RuleBase:RU364106};
GN ORFNames=BleG1_1997 {ECO:0000313|EMBL:AIC94575.1};
OS Shouchella lehensis G1.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Shouchella.
OX NCBI_TaxID=1246626 {ECO:0000313|EMBL:AIC94575.1, ECO:0000313|Proteomes:UP000027142};
RN [1] {ECO:0000313|EMBL:AIC94575.1, ECO:0000313|Proteomes:UP000027142}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=G1 {ECO:0000313|EMBL:AIC94575.1,
RC ECO:0000313|Proteomes:UP000027142};
RX PubMed=24811681; DOI=10.1016/j.gene.2014.05.012;
RA Noor Y.M., Samsulrizal N.H., Jema'on N.A., Low K.O., Ramli A.N.,
RA Alias N.I., Damis S.I., Fuzi S.F., Isa M.N., Murad A.M., Raih M.F.,
RA Bakar F.D., Najimudin N., Mahadi N.M., Illias R.M.;
RT "A comparative genomic analysis of the alkalitolerant soil bacterium
RT Bacillus lehensis G1.";
RL Gene 545:253-261(2014).
CC -!- FUNCTION: 3'-5' exonuclease. {ECO:0000256|HAMAP-Rule:MF_02206,
CC ECO:0000256|RuleBase:RU364106}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665};
CC -!- SIMILARITY: Belongs to the helicase family. DinG subfamily. Type 2 sub-
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_02206,
CC ECO:0000256|RuleBase:RU364106}.
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DR EMBL; CP003923; AIC94575.1; -; Genomic_DNA.
DR RefSeq; WP_038480167.1; NZ_CP003923.1.
DR AlphaFoldDB; A0A060LXU0; -.
DR STRING; 1246626.BleG1_1997; -.
DR KEGG; ble:BleG1_1997; -.
DR PATRIC; fig|1246626.3.peg.1997; -.
DR eggNOG; COG0847; Bacteria.
DR eggNOG; COG1199; Bacteria.
DR HOGENOM; CLU_012117_1_0_9; -.
DR OrthoDB; 9803913at2; -.
DR Proteomes; UP000027142; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
DR GO; GO:0016818; F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR CDD; cd06127; DEDDh; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR HAMAP; MF_02206; DinG_exonucl; 1.
DR InterPro; IPR006555; ATP-dep_Helicase_C.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR006310; DinG.
DR InterPro; IPR045028; DinG/Rad3-like.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR014013; Helic_SF1/SF2_ATP-bd_DinG/Rad3.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR010614; RAD3-like_helicase_DEAD.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR01407; dinG_rel; 1.
DR NCBIfam; TIGR00573; dnaq; 1.
DR PANTHER; PTHR11472; DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER; 1.
DR PANTHER; PTHR11472:SF47; FANCONI ANEMIA GROUP J PROTEIN; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF06733; DEAD_2; 1.
DR Pfam; PF13307; Helicase_C_2; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00491; HELICc2; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS51193; HELICASE_ATP_BIND_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_02206};
KW Exonuclease {ECO:0000256|HAMAP-Rule:MF_02206,
KW ECO:0000256|RuleBase:RU364106};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:AIC94575.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_02206};
KW Nuclease {ECO:0000256|HAMAP-Rule:MF_02206, ECO:0000256|RuleBase:RU364106};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_02206}; Reference proteome {ECO:0000313|Proteomes:UP000027142}.
FT DOMAIN 253..532
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51193"
FT MOTIF 468..471
FT /note="DEAH box"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02206"
FT BINDING 288..295
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02206"
SQ SEQUENCE 928 AA; 105690 MW; 83075AD02013FB4A CRC64;
MSQSYVVLDL ETTGNAPEQG ARIIQIGAVK VEGHKITDRF STFVDPQCEI PPFITELTGI
TEKDVSGAPL FEEVAEEVLT FLDGSHFVAH NVPFDKGFLK KQLALAGYQF PNCYSFDTVE
LSRGLLPTLD SYKLSDLTVY FDMDHSRPHQ ADSDAEMTAE LFIYLHNKIL QLPLPTLQAL
KKTTASLKSD WDFLIEEGIQ QALKKSQFED ASIDVFRQLA LKRIEDEEEE FIEEELPETY
SVFEQAYFSE KGKLASQFEQ YEIREGQKEM ASAVYEAFSD HAHTLIEAGT GTGKTLGYLI
PSVYYAKQTN QKVVLSTYSI PLQEQLLRKD LALMKQASGI QVRVAVLKGR QHYLDLRKFE
EAALYSEEGS YDVQLTKAQI LIWLLETQTG DVEELNLSSG GQLFWQTIQS DASSDLGRFN
PWFTRCFYHR SRKQAKEADI IIVNHALLMT DVHQRRAIIP TYSHAVIDEA HHLEETAIDY
FGLAVNYLSI GFSLSRLVQS VTQNKSFMNE SEGFISVLNE IKEDVEELFR MLHAFVSERQ
TEATDVGRMS YVYRSFQEEG VLWQGILECA MRVQMNSSSV IEQLYSAIDK EKDNEPAYEE
RSFIANVKSM VALFEEEVTQ LYELLLEYDE EFVYWIEAEP KGAKNAIYLY AKPIRVNERL
ADDFFATKQS VVLTSATLTV NQSFDYAQKR LGLEDFGVRT KKIPSPFSYE KQLKVFIPTD
IPDVRGHNDQ LFIQDVAIKL WRLTESTNKK ALVLFTSYDM LKQVYQYVKD LDDEGQITLI
GQGVTSGSRS RLLKLFRQAN GASILLGTNS FWEGIDLAGD ALEHLIIVRL PFAPPNQPLT
RAQINAAKAN GENPFTDLSL PQAVIRFRQG FGRLIRTAKD QGNFFIFDRR VVTTRYGKTF
LASLPTTPVY EGKFEHLLEA HIHNQEEE
//
