ID A0A060M1F8_9BACI Unreviewed; 757 AA.
AC A0A060M1F8;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE SubName: Full=Minor extracellular protease {ECO:0000313|EMBL:AIC96267.1};
GN ORFNames=BleG1_3720 {ECO:0000313|EMBL:AIC96267.1};
OS Shouchella lehensis G1.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Shouchella.
OX NCBI_TaxID=1246626 {ECO:0000313|EMBL:AIC96267.1, ECO:0000313|Proteomes:UP000027142};
RN [1] {ECO:0000313|EMBL:AIC96267.1, ECO:0000313|Proteomes:UP000027142}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=G1 {ECO:0000313|EMBL:AIC96267.1,
RC ECO:0000313|Proteomes:UP000027142};
RX PubMed=24811681; DOI=10.1016/j.gene.2014.05.012;
RA Noor Y.M., Samsulrizal N.H., Jema'on N.A., Low K.O., Ramli A.N.,
RA Alias N.I., Damis S.I., Fuzi S.F., Isa M.N., Murad A.M., Raih M.F.,
RA Bakar F.D., Najimudin N., Mahadi N.M., Illias R.M.;
RT "A comparative genomic analysis of the alkalitolerant soil bacterium
RT Bacillus lehensis G1.";
RL Gene 545:253-261(2014).
CC -!- SIMILARITY: Belongs to the peptidase S8 family.
CC {ECO:0000256|ARBA:ARBA00011073, ECO:0000256|PROSITE-ProRule:PRU01240,
CC ECO:0000256|RuleBase:RU003355}.
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DR EMBL; CP003923; AIC96267.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A060M1F8; -.
DR STRING; 1246626.BleG1_3720; -.
DR KEGG; ble:BleG1_3720; -.
DR PATRIC; fig|1246626.3.peg.3714; -.
DR eggNOG; COG1404; Bacteria.
DR HOGENOM; CLU_004945_1_0_9; -.
DR Proteomes; UP000027142; Chromosome.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd02133; PA_C5a_like; 1.
DR CDD; cd07474; Peptidases_S8_subtilisin_Vpr-like; 1.
DR Gene3D; 3.50.30.30; -; 1.
DR Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 1.
DR InterPro; IPR046450; PA_dom_sf.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR InterPro; IPR022398; Peptidase_S8_His-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034213; S8_Vpr-like.
DR PANTHER; PTHR43806:SF59; CEREVISIN-RELATED; 1.
DR PANTHER; PTHR43806; PEPTIDASE S8; 1.
DR Pfam; PF02225; PA; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52025; PA domain; 1.
DR SUPFAM; SSF52743; Subtilisin-like; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00136; SUBTILASE_ASP; 1.
DR PROSITE; PS00137; SUBTILASE_HIS; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 3: Inferred from homology;
KW Cell wall {ECO:0000256|ARBA:ARBA00022512};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU01240};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Reference proteome {ECO:0000313|Proteomes:UP000027142};
KW Secreted {ECO:0000256|ARBA:ARBA00022512};
KW Serine protease {ECO:0000256|ARBA:ARBA00022825, ECO:0000256|PROSITE-
KW ProRule:PRU01240}.
FT DOMAIN 143..537
FT /note="Peptidase S8/S53"
FT /evidence="ECO:0000259|Pfam:PF00082"
FT DOMAIN 351..420
FT /note="PA"
FT /evidence="ECO:0000259|Pfam:PF02225"
FT ACT_SITE 152
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 192
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 485
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
SQ SEQUENCE 757 AA; 82127 MW; 0B5BB6644C8D1A45 CRC64;
MTKRGTVYRL LLGILSVLIL LQGMSMAMGV NGVALAAENQ QPTIDSSKRV VIVSASGDVN
RAVDVVENRI SSAMIRNIYE NVYGGFSLEI AEKDIERLKQ LSVIARVDDV AHYQPSLEGS
IPFIGAGDRL MDKQDGKERL LTGKGVKVGV IDTGVDYKHP DLEANYRGGY DIVDQDDDPM
ETKKSQGEPT LHGTHVAGII AANGRIKGVA PEAEIYAYRA LGPGGQGTTE HILEAIERAF
KDDVDVINLS LGNTVNGPDW PTSLALDRVV EKGIVAVTSN GNSGPSMWSV GSPGTSTKAI
SVGASLPPVE VPVINVNGLK DELTLTPILG AKSWQLKASF ELVDVGHGLL TDYKGKDVKD
KLVLMKRGRS NFAEKLALAS KAGAKGVLIY NNLEGAFAGA VTEPIPITGA TLSKTEGEAL
RSLLKQRKHK VKVDTQYKVK QDLMAPFSSR GPVTETWEIK PDLVAPGVAI QSTVPKGYLD
LNGTSMASPH VAGAAALLIE QHPDWSPEQV KSALLNSTKR LQDEQGKEYL PFEQGAGRLQ
VDKAIQTRTL ISPATLSFGE LEKGEGVVEK EVTISIENIS KSTKEYTIDP PIGEVDGISW
EFEEKVTVKG QSKKAFTLQA TIDQHAIQKG LHTGYITVRD KDEDIHLPYM LFIEEPNYPR
VMAFQMAHSD KANGYYYEYY LPGGAEESSV HLFDPDTLEF VATIIENQDQ GRGMVNGEID
NLQVAPGRYK ALIYAKNEGK TDILEAMIHI GEEWIGP
//
