ID A0A061B182_CYBFA Unreviewed; 552 AA.
AC A0A061B182;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE RecName: Full=Malate synthase {ECO:0000256|ARBA:ARBA00012636, ECO:0000256|RuleBase:RU000555};
DE EC=2.3.3.9 {ECO:0000256|ARBA:ARBA00012636, ECO:0000256|RuleBase:RU000555};
GN ORFNames=BON22_5359 {ECO:0000313|EMBL:ONH64796.1}, CYFA0S_12e01442g
GN {ECO:0000313|EMBL:CDR43522.1};
OS Cyberlindnera fabianii (Yeast) (Hansenula fabianii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Phaffomycetaceae; Cyberlindnera.
OX NCBI_TaxID=36022 {ECO:0000313|EMBL:CDR43522.1};
RN [1] {ECO:0000313|EMBL:CDR43522.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=YJS4271 {ECO:0000313|EMBL:CDR43522.1};
RX PubMed=25103752; DOI=10.1128/genomeA.00638-14;
RA Freel K.C., Sarilar V., Neuveglise C., Devillers H., Friedrich A.,
RA Schacherer J.;
RT "Genome sequence of the yeast Cyberlindnera fabianii (Hansenula
RT fabianii).";
RL Genome Announc. 2:E00638-E00638(2014).
RN [2] {ECO:0000313|Proteomes:UP000189513}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=65 {ECO:0000313|Proteomes:UP000189513};
RX PubMed=28385833; DOI=10.1128/genomeA.00064-17;
RA van Rijswijck I.M.H., Derks M.F.L., Abee T., de Ridder D., Smid E.J.;
RT "Genome sequences of Cyberlindnera fabianii 65, Pichia kudriavzevii 129,
RT and Saccharomyces cerevisiae 131 isolated from fermented masau fruits in
RT Zimbabwe.";
RL Genome Announc. 5:E00064-E00064(2017).
RN [3] {ECO:0000313|EMBL:ONH64796.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=65 {ECO:0000313|EMBL:ONH64796.1};
RA Mah S.A., Swanson W.J., Moy G.W., Vacquier V.D.;
RL Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00001699,
CC ECO:0000256|RuleBase:RU000555};
CC -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC isocitrate: step 2/2. {ECO:0000256|RuleBase:RU000555}.
CC -!- SIMILARITY: Belongs to the malate synthase family.
CC {ECO:0000256|ARBA:ARBA00006394, ECO:0000256|RuleBase:RU000555}.
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DR EMBL; LK052897; CDR43522.1; -; Genomic_DNA.
DR EMBL; MPUK01000017; ONH64796.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A061B182; -.
DR STRING; 36022.A0A061B182; -.
DR VEuPathDB; FungiDB:BON22_5359; -.
DR OMA; AGCDSCW; -.
DR OrthoDB; 177378at2759; -.
DR UniPathway; UPA00703; UER00720.
DR Proteomes; UP000189513; Unassembled WGS sequence.
DR GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR CDD; cd00727; malate_synt_A; 1.
DR Gene3D; 3.20.20.360; Malate synthase, domain 3; 1.
DR Gene3D; 1.20.1220.12; Malate synthase, domain III; 1.
DR InterPro; IPR044856; Malate_synth_C_sf.
DR InterPro; IPR011076; Malate_synth_sf.
DR InterPro; IPR006252; Malate_synthA.
DR InterPro; IPR019830; Malate_synthase_CS.
DR InterPro; IPR001465; Malate_synthase_TIM.
DR InterPro; IPR048355; MS_C.
DR InterPro; IPR048356; MS_N.
DR InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR NCBIfam; TIGR01344; malate_syn_A; 1.
DR PANTHER; PTHR42902; MALATE SYNTHASE; 1.
DR PANTHER; PTHR42902:SF1; MALATE SYNTHASE 1-RELATED; 1.
DR Pfam; PF20659; MS_C; 1.
DR Pfam; PF20656; MS_N; 1.
DR Pfam; PF01274; MS_TIM-barrel; 1.
DR PIRSF; PIRSF001363; Malate_synth; 1.
DR SUPFAM; SSF51645; Malate synthase G; 1.
DR PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Glyoxylate bypass {ECO:0000256|ARBA:ARBA00022435,
KW ECO:0000256|RuleBase:RU000555};
KW Reference proteome {ECO:0000313|Proteomes:UP000189513};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000555};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532,
KW ECO:0000256|RuleBase:RU000555}.
FT DOMAIN 17..79
FT /note="Malate synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF20656"
FT DOMAIN 171..417
FT /note="Malate synthase TIM barrel"
FT /evidence="ECO:0000259|Pfam:PF01274"
FT DOMAIN 425..540
FT /note="Malate synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF20659"
FT ACT_SITE 175
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
FT ACT_SITE 459
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
SQ SEQUENCE 552 AA; 62241 MW; F9095C736D190F3D CRC64;
MTVADNPFPE TQDLLQGVQV RARIPANAKH IVTPEALAFI AVLHRTFNAT RKKLLQNRQV
EQAKRDSGIL PNFHPDTAYI RDDPTWKGPS LGPKLQDRRT EITGPSSRSM LVNALNAHVA
TYMCDMEDSA TPTWLNVIDG QVNLFDAVRK QIDFKKGNKE YKLNLNKHIP TLIVRPRGWH
LEEKHILVDG EPTSGSIVDF ALYFFHNAKE TIAQGFGPYF YLPKMEHHLE AKLWNDIFNV
SQDYIGIPRG TIRATVLIET LPAAFQMDEI IYQLRDHSAG LNCGRWDYIF SYIKCLRNHR
EFILPDRAQV NMSVGFMASY VKLLIYTCHK RGVHAMGGMA AQIPIKDDPK RNAIAMENVK
KDKLREFNAG HDGTWIAHPA LAPVANEIFK KMLTPNQINS TVGIEGYVPI TQRDLLSPYV
EGGAITEAGI RANLEIGLSY IEAWLRGVGC VPINYLMEDA ATAEVSRSQL YQWVHHGAKT
VDGKTITPEY TSKLLTEVVE GLAAKAKQGH KFYQAANYFK DDVTGKKYHD FLTTLIYDDV
TTIERPLPAT KL
//
