ID A0A061FEM7_THECC Unreviewed; 645 AA.
AC A0A061FEM7;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=rhamnogalacturonan endolyase {ECO:0000256|ARBA:ARBA00012437};
DE EC=4.2.2.23 {ECO:0000256|ARBA:ARBA00012437};
GN ORFNames=TCM_046891 {ECO:0000313|EMBL:EOY15516.1};
OS Theobroma cacao (Cacao) (Cocoa).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Byttnerioideae; Theobroma.
OX NCBI_TaxID=3641 {ECO:0000313|EMBL:EOY15516.1, ECO:0000313|Proteomes:UP000026915};
RN [1] {ECO:0000313|EMBL:EOY15516.1, ECO:0000313|Proteomes:UP000026915}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Matina 1-6 {ECO:0000313|Proteomes:UP000026915};
RX PubMed=23731509; DOI=10.1186/gb-2013-14-6-r53;
RA Motamayor J.C., Mockaitis K., Schmutz J., Haiminen N., Iii D.L.,
RA Cornejo O., Findley S.D., Zheng P., Utro F., Royaert S., Saski C.,
RA Jenkins J., Podicheti R., Zhao M., Scheffler B.E., Stack J.C., Feltus F.A.,
RA Mustiga G.M., Amores F., Phillips W., Marelli J.P., May G.D., Shapiro H.,
RA Ma J., Bustamante C.D., Schnell R.J., Main D., Gilbert D., Parida L.,
RA Kuhn D.N.;
RT "The genome sequence of the most widely cultivated cacao type and its use
RT to identify candidate genes regulating pod color.";
RL Genome Biol. 14:R53.1-R53.24(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-
CC (1->4)-alpha-D-galactopyranosyluronic acid bonds of
CC rhamnogalacturonan I domains in ramified hairy regions of pectin
CC leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-
CC unsaturated D-galactopyranosyluronic acid at the non-reducing end.;
CC EC=4.2.2.23; Evidence={ECO:0000256|ARBA:ARBA00001324};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the polysaccharide lyase 4 family.
CC {ECO:0000256|ARBA:ARBA00010418}.
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DR EMBL; CM001886; EOY15516.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A061FEM7; -.
DR STRING; 3641.A0A061FEM7; -.
DR EnsemblPlants; EOY15516; EOY15516; TCM_046891.
DR Gramene; EOY15516; EOY15516; TCM_046891.
DR eggNOG; ENOG502QQM5; Eukaryota.
DR HOGENOM; CLU_015099_1_0_1; -.
DR InParanoid; A0A061FEM7; -.
DR OMA; DCAANET; -.
DR Proteomes; UP000026915; Chromosome 8.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0102210; F:rhamnogalacturonan endolyase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR CDD; cd10317; RGL4_C; 1.
DR CDD; cd10316; RGL4_M; 1.
DR CDD; cd10320; RGL4_N; 1.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 2.60.40.1120; Carboxypeptidase-like, regulatory domain; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR InterPro; IPR013784; Carb-bd-like_fold.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR029413; RG-lyase_II.
DR InterPro; IPR029411; RG-lyase_III.
DR InterPro; IPR010325; Rhamnogal_lyase.
DR PANTHER; PTHR32018:SF1; RHAMNOGALACTURONAN ENDOLYASE; 1.
DR PANTHER; PTHR32018; RHAMNOGALACTURONATE LYASE FAMILY PROTEIN; 1.
DR Pfam; PF14683; CBM-like; 1.
DR Pfam; PF14686; fn3_3; 1.
DR Pfam; PF06045; Rhamnogal_lyase; 1.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF49452; Starch-binding domain-like; 1.
PE 3: Inferred from homology;
KW Cell wall biogenesis/degradation {ECO:0000256|ARBA:ARBA00023316};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|EMBL:EOY15516.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000026915};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 359..424
FT /note="Rhamnogalacturonan lyase"
FT /evidence="ECO:0000259|Pfam:PF14686"
FT DOMAIN 445..633
FT /note="Rhamnogalacturonan lyase"
FT /evidence="ECO:0000259|Pfam:PF14683"
SQ SEQUENCE 645 AA; 73878 MW; AD8605350017E584 CRC64;
MSSRGVQLDV QDKYVVMDND ILQVTISNPG GIVTGLQYNG IDNLLAVEND ETDRGYWDLV
WNLAGRKGTK GKFDRIEATS FNVIVENEEQ VELSFTRMWD SSLEGRVVPL NIDKRFVMLR
NSSGFYTYAI YEHLEEWPAF ILDRFRVAFK LRKDKFHYMA MADNRQRCMP LPDDRLPYRS
QTLAYPEAVL LVDPLEPEFR GEVDDKYQYS CENKDNRVHG WICTDPPVPV GFWQITPSDE
FRSAGPLKQN LTSHVGPTTL AVMHSVHYSG EDLLLKFGTN EPWKKVFGPI FIYLNSLSNG
GDPLSLWEDA KEQMTIEVQG WPYTFPVSED FPQSDQRGNV SGRLLVKDRY VHDDNIPANG
AYVGLAPPGD VGSWQREVKG YQFWTKTDED GYFCINNIRT GDYNLYAWVP GFIGDYRYNV
IITPTAGYDI YMGDLVYEPP RDGPTLWEIG IPDRTAAEFY VPDPSPVYIN KLYVNHQDRF
RQYGLWERYA ELYPDGDLVY TVGVSDYTKD WFFAQVTRKK DDNTYQGTTW QIKFKLDCVD
ESGTYKLRLA LASAHASELQ VRVNDPDAIS PLFSSGQIGK DNTIARHGIH GLYWLYNVDI
PGNLLHEGDN TIFLTQSKST SPFQGIMYDY IRLEGPPSSD ANKRP
//
