ID A0A061HHV5_BLUGR Unreviewed; 547 AA.
AC A0A061HHV5;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=guanosine-diphosphatase {ECO:0000256|ARBA:ARBA00038903};
DE EC=3.6.1.42 {ECO:0000256|ARBA:ARBA00038903};
GN ORFNames=BGT96224_1025 {ECO:0000313|EMBL:EPQ65077.1},
GN BGT96224V2_LOCUS3136 {ECO:0000313|EMBL:SUZ09980.1};
OS Blumeria graminis f. sp. tritici 96224.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Erysiphales; Erysiphaceae; Blumeria.
OX NCBI_TaxID=1268274 {ECO:0000313|EMBL:SUZ09980.1};
RN [1] {ECO:0000313|Proteomes:UP000053110}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=96224 {ECO:0000313|Proteomes:UP000053110};
RX PubMed=23852167; DOI=10.1038/ng.2704;
RA Wicker T., Oberhaensli S., Parlange F., Buchmann J.P., Shatalina M.,
RA Roffler S., Ben-David R., Dolezel J., Simkova H., Schulze-Lefert P.,
RA Spanu P.D., Bruggmann R., Amselem J., Quesneville H.,
RA Ver Loren van Themaat E., Paape T., Shimizu K.K., Keller B.;
RT "The wheat powdery mildew genome shows the unique evolution of an obligate
RT biotroph.";
RL Nat. Genet. 45:1092-1096(2013).
RN [2] {ECO:0000313|EMBL:EPQ65077.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=96224 {ECO:0000313|EMBL:EPQ65077.1};
RA Oberhaensli S., Wicker T., Keller B.;
RT "The wheat powdery mildew genome reveals unique evolution of an obligate
RT biotroph.";
RL Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:SUZ09980.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=96224 {ECO:0000313|EMBL:SUZ09980.1};
RA Quirk P.G., Krulwich T.A.;
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: After transfer of sugars to endogenous macromolecular
CC acceptors, the enzyme converts nucleoside diphosphates to nucleoside
CC monophosphates which in turn exit the Golgi lumen in a coupled
CC antiporter reaction, allowing entry of additional nucleotide sugar from
CC the cytosol. {ECO:0000256|ARBA:ARBA00037742}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000256|ARBA:ARBA00004323}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004323}.
CC -!- SIMILARITY: Belongs to the GDA1/CD39 NTPase family.
CC {ECO:0000256|ARBA:ARBA00009283, ECO:0000256|RuleBase:RU003833}.
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DR EMBL; KE375040; EPQ65077.1; -; Genomic_DNA.
DR EMBL; UIGY01000067; SUZ09980.1; -; Genomic_DNA.
DR HOGENOM; CLU_010246_4_0_1; -.
DR OrthoDB; 180318at2759; -.
DR Proteomes; UP000053110; Unassembled WGS sequence.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004382; F:GDP phosphatase activity; IEA:EnsemblFungi.
DR GO; GO:0045134; F:UDP phosphatase activity; IEA:EnsemblFungi.
DR GO; GO:0006486; P:protein glycosylation; IEA:EnsemblFungi.
DR CDD; cd00012; NBD_sugar-kinase_HSP70_actin; 1.
DR Gene3D; 3.30.420.40; -; 1.
DR Gene3D; 3.30.420.150; Exopolyphosphatase. Domain 2; 1.
DR InterPro; IPR000407; GDA1_CD39_NTPase.
DR PANTHER; PTHR11782; ADENOSINE/GUANOSINE DIPHOSPHATASE; 1.
DR PANTHER; PTHR11782:SF83; NTPASE, ISOFORM F; 1.
DR Pfam; PF01150; GDA1_CD39; 1.
DR PROSITE; PS01238; GDA1_CD39_NTPASE; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|PIRSR:PIRSR600407-2};
KW Hydrolase {ECO:0000256|RuleBase:RU003833};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|PIRSR:PIRSR600407-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000053110};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 53..71
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT ACT_SITE 243
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR600407-1"
FT BINDING 274..278
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PIRSR:PIRSR600407-2"
SQ SEQUENCE 547 AA; 60779 MW; 16697E7C797E002E CRC64;
MRRISVSQKS KEVEGDPHEK LARYQTSGNS AAAIGAQERA KIVWMPQTQR SRYVRTGGVL
LFVVFLFYYF LPHRTSFYGE VTKLENAPVP SDSFTETKKC SKPYEKSKPL IQYVLMIDAG
STGSRIHVYK FNNCGPTPEL EQEEFKMTEK KSGGSGLSSY KTDADGAAKS LDVLLDVAMK
NVPDKYKACS PIAVKATAGL RLLGPEMSQK ILDAVRTRLE TAYPFPVVSS AENGVAIMDG
KDEGVYAWIT TNYLLGKIGG SDQRKTAAIL DLGGGSTQIV FEPDYQSLAP DKLKEGEHKY
NLSFGGRVYE LYQHSHLGYG LMSAREAIHK TLIEDIHIGN PEDSAWIFRP IINPCIAPGM
SRMVDIKLED NHQLGKKLSV NMTGPSSPAP AQCRRLAEKV LHKDSVCDLT PCSFNGVYQP
PLSKTFSQED IYTFSYFYDR INVLGMPDSF TLNELHRLTE QVCGGESDWE LFSAVPGALA
ELQGRPEHCL DLNYIVALLH TGYEMPIERE IKIAKKIDNN ELGWCLGASL PLLGERSGWE
CRVKEVS
//
