ID A0A062UVG7_9EURY Unreviewed; 356 AA.
AC A0A062UVG7;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE SubName: Full=Pyruvate-formate lyase-activating enzyme {ECO:0000313|EMBL:KCZ71011.1};
DE EC=1.97.1.4 {ECO:0000313|EMBL:KCZ71011.1};
GN ORFNames=ANME2D_03040 {ECO:0000313|EMBL:KCZ71011.1};
OS Candidatus Methanoperedens nitroreducens.
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Candidatus Methanoperedenaceae; Methanoperedens.
OX NCBI_TaxID=1392998 {ECO:0000313|EMBL:KCZ71011.1, ECO:0000313|Proteomes:UP000027153};
RN [1] {ECO:0000313|EMBL:KCZ71011.1, ECO:0000313|Proteomes:UP000027153}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ANME-2d {ECO:0000313|EMBL:KCZ71011.1,
RC ECO:0000313|Proteomes:UP000027153};
RX PubMed=23892779; DOI=10.1038/nature12375;
RA Haroon M.F., Hu S., Shi Y., Imelfort M., Keller J., Hugenholtz P., Yuan Z.,
RA Tyson G.W.;
RT "Anaerobic oxidation of methane coupled to nitrate reduction in a novel
RT archaeal lineage.";
RL Nature 500:567-570(2013).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KCZ71011.1}.
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DR EMBL; JMIY01000007; KCZ71011.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A062UVG7; -.
DR Proteomes; UP000027153; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0043365; F:[formate-C-acetyltransferase]-activating enzyme activity; IEA:UniProtKB-EC.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd00207; fer2; 1.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.10.20.740; -; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR036010; 2Fe-2S_ferredoxin-like_sf.
DR InterPro; IPR001041; 2Fe-2S_ferredoxin-type.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR034457; Organic_radical-activating.
DR InterPro; IPR007197; rSAM.
DR PANTHER; PTHR30352; PYRUVATE FORMATE-LYASE-ACTIVATING ENZYME; 1.
DR Pfam; PF13510; Fer2_4; 1.
DR Pfam; PF13353; Fer4_12; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SFLD; SFLDG01067; SPASM/twitch_domain_containing; 1.
DR SUPFAM; SSF54292; 2Fe-2S ferredoxin-like; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Lyase {ECO:0000313|EMBL:KCZ71011.1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000313|EMBL:KCZ71011.1};
KW Pyruvate {ECO:0000313|EMBL:KCZ71011.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000027153};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 116..353
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 356 AA; 39660 MW; 9C0307433B3070D6 CRC64;
MLACSGEAIE MVEDKRQRNT IKFEFNNRVL EAPERITIKK ALEYAGIRFS ELPDGLYSPC
NAGGCYCCAV SADGEVVPSC ITEIKDGMKL SPASKPLRVV TGFVPHMVGG VGTPYYLKSY
SSYIEVACFT HGCNFRCPQC QNNVMAFTGG MAISPETAAE VLTSVRSMYK VNRMAFSGGE
CTLNRQWLTG AIRHLKELNK DKKARIHVDT NASIFTKDYL EELVLAGMTD IGIDIKSLHL
DTFEKITGTD GKTAKTYLEN EWFSARYMIE NHPEVFTGIG IPYNKALISK EEIYDMGLRI
ADISKDVQVC VLDYRRAFRR EMAAPDIQEM KEVKEILNST GLKTVIAQTM KGNIVP
//
