ID A0A062VCZ0_9EURY Unreviewed; 253 AA.
AC A0A062VCZ0;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=CRISPR-associated endoribonuclease {ECO:0000256|PIRNR:PIRNR005054};
GN ORFNames=ANME2D_00577 {ECO:0000313|EMBL:KCZ73509.1};
OS Candidatus Methanoperedens nitroreducens.
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Candidatus Methanoperedenaceae; Methanoperedens.
OX NCBI_TaxID=1392998 {ECO:0000313|EMBL:KCZ73509.1, ECO:0000313|Proteomes:UP000027153};
RN [1] {ECO:0000313|EMBL:KCZ73509.1, ECO:0000313|Proteomes:UP000027153}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ANME-2d {ECO:0000313|EMBL:KCZ73509.1,
RC ECO:0000313|Proteomes:UP000027153};
RX PubMed=23892779; DOI=10.1038/nature12375;
RA Haroon M.F., Hu S., Shi Y., Imelfort M., Keller J., Hugenholtz P., Yuan Z.,
RA Tyson G.W.;
RT "Anaerobic oxidation of methane coupled to nitrate reduction in a novel
RT archaeal lineage.";
RL Nature 500:567-570(2013).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat), is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain sequences complementary to
CC antecedent mobile elements and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA).
CC {ECO:0000256|PIRNR:PIRNR005054}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas6/Cse3/CasE
CC family. {ECO:0000256|ARBA:ARBA00005937, ECO:0000256|PIRNR:PIRNR005054}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KCZ73509.1}.
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DR EMBL; JMIY01000001; KCZ73509.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A062VCZ0; -.
DR PATRIC; fig|1392998.3.peg.185; -.
DR Proteomes; UP000027153; Unassembled WGS sequence.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.1890; -; 1.
DR Gene3D; 3.30.70.1900; -; 1.
DR InterPro; IPR049435; Cas_Cas6_C.
DR InterPro; IPR010156; CRISPR-assoc_prot_Cas6.
DR InterPro; IPR045747; CRISPR-assoc_prot_Cas6_N_sf.
DR NCBIfam; TIGR01877; cas_cas6; 1.
DR PANTHER; PTHR36984; CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1; 1.
DR PANTHER; PTHR36984:SF1; CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1; 1.
DR Pfam; PF21350; Cas6_I-A; 1.
DR Pfam; PF01881; Cas_Cas6_C; 1.
DR PIRSF; PIRSF005054; PF1131; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW Reference proteome {ECO:0000313|Proteomes:UP000027153};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884}.
FT DOMAIN 124..245
FT /note="CRISPR associated protein Cas6 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF01881"
FT ACT_SITE 34
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005054-50"
FT ACT_SITE 49
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005054-50"
FT SITE 55
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR005054-1"
SQ SEQUENCE 253 AA; 29497 MW; 3E18C1451BD4325E CRC64;
MKRRNKLRSK ITIRNLSNLP VNFDYQYGLA SMLYFKLADA DVRLANEIHA HQGFKFYTFS
NLVLMNRKAC KSGLCFERAY FILSSPDDRF IRSFTEGLLQ QPEFYLDGPR GRIDLVIERI
EILKEPSFGD ECLFRTLSPI YIKTIRKENE CLREIDLYPT EPKFYENLHR NLTERFSEFY
GKEPVDHFDV VQTADINPRR IKIADSYRRC SLMSLRIQAS PELLKFAYDA GLGEKNAMGF
GCVDVVQGSK PIQ
//
