ID A0A063BI15_9BURK Unreviewed; 787 AA.
AC A0A063BI15;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN ORFNames=LIG30_3554 {ECO:0000313|EMBL:KDB07308.1};
OS Burkholderia sp. lig30.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia.
OX NCBI_TaxID=1192124 {ECO:0000313|EMBL:KDB07308.1, ECO:0000313|Proteomes:UP000027020};
RN [1] {ECO:0000313|EMBL:KDB07308.1, ECO:0000313|Proteomes:UP000027020}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LIG30 {ECO:0000313|Proteomes:UP000027020};
RA Woo H.L., Utturkar S.M., Klingeman D.M., Simmons B.A., DeAngelis K.M.,
RA Brown S.D., Hazen T.C.;
RT "Draft Genome of the Lignin-degrading Burkholderia sp. Strain LIG30,
RT Isolated from Wet Tropical Forest Soil.";
RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KDB07308.1}.
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DR EMBL; JGVW01000087; KDB07308.1; -; Genomic_DNA.
DR RefSeq; WP_038713924.1; NZ_JGVW01000087.1.
DR AlphaFoldDB; A0A063BI15; -.
DR STRING; 1192124.LIG30_3554; -.
DR PATRIC; fig|1192124.4.peg.3613; -.
DR eggNOG; COG0210; Bacteria.
DR OrthoDB; 5905204at2; -.
DR Proteomes; UP000027020; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000027020}.
FT DOMAIN 6..283
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 284..598
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 27..34
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 787 AA; 86921 MW; 550F0F3AC6F4ED33 CRC64;
MPDLLANLNP EQYAAVTLPN ELALILAGAG SGKTRVLITR IAWLIQHGYA SPATVLAVTF
TNKAAREMMA RLSAMMPIDT RGMWIGTFHG LCNRMLRAHY RDAGLPQTFQ ILDTADQLSA
IKRLMKAANV DDEKYPPKNV QYFINNAKEQ GLRPDKVDAT DSFNRKFVEL YQAYEQQCQR
EGVVDFPELL LRCYELLAHN PPLRAHYQAR FRHILVDEFQ DTNKLQYAWL KLLAGSQNAI
FAVGDDDQSI YAFRGANVGN MRDFENEFRV RNLIKLEQNY RSHGNILDAA NHLISNNAHR
LGKNLRTDAG HGEPVRVYEA ATDSQEAGWI VEEIRSLISA GMVRSEIAIL YRSNAQSRAI
EHTLMTAGIP YRVYGGLRFF ERQEIKHALA YLRLIDNPND DTAFARVVNF PTRGIGARSI
EQLADAARLY NCAMAAAIPY VTGKAGSSLG AFANLVAKMR ADTQQMSLPE TVEYVVRASG
LAEFYQGERE GQDRLENLQE LVNAATAFVS EEGYGLDTPA RSIPLRAGAI AAPEIAASQR
DPADAVLDPA ALGDPAQNPD TMTPLAGFLS HASLEAGDNQ AQAGQDAVQL MTVHAAKGLE
FAAVFITGLE EGLFPHENSA VEAAGLEEER RLMYVAITRA KERLYLSFAQ SRMLHGQTRY
NVRSRFFDEL PQHVLKWLTP KVEAGARWGG RSDNAGWGRD WFARQGGGGR EQVVDAAVSA
PLPAFANQQR AAESGFRVGQ QVFHTKFGEG TVTALEGSGA DAKAQVKFKR HGEKWLALAV
AKLQTVE
//
