ID A0A066RLH0_9GAMM Unreviewed; 499 AA.
AC A0A066RLH0;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Methionyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00030904};
GN ORFNames=EA58_18750 {ECO:0000313|EMBL:KDM89986.1};
OS Photobacterium galatheae.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Photobacterium.
OX NCBI_TaxID=1654360 {ECO:0000313|EMBL:KDM89986.1, ECO:0000313|Proteomes:UP000027192};
RN [1] {ECO:0000313|EMBL:KDM89986.1, ECO:0000313|Proteomes:UP000027192}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S2753 {ECO:0000313|EMBL:KDM89986.1,
RC ECO:0000313|Proteomes:UP000027192};
RA Machado H.R., Gram L.;
RT "Draft genome sequence of Photobacterium halotolerans S2753: a solonamide,
RT ngercheumicin and holomycin producer.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-
CC methionyl-tRNA(Met); Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667,
CC Rhea:RHEA-COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530,
CC ChEBI:CHEBI:456215; EC=6.1.1.10;
CC Evidence={ECO:0000256|ARBA:ARBA00001234};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|RuleBase:RU363039}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KDM89986.1}.
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DR EMBL; JMIB01000038; KDM89986.1; -; Genomic_DNA.
DR RefSeq; WP_036756073.1; NZ_JMIB01000038.1.
DR AlphaFoldDB; A0A066RLH0; -.
DR STRING; 1654360.EA58_18750; -.
DR OrthoDB; 9810191at2; -.
DR Proteomes; UP000027192; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:InterPro.
DR GO; GO:0006431; P:methionyl-tRNA aminoacylation; IEA:InterPro.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 2.20.28.20; Methionyl-tRNA synthetase, Zn-domain; 1.
DR InterPro; IPR023458; Met-tRNA_ligase_1.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR033911; MetRS_core.
DR InterPro; IPR029038; MetRS_Zn.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR45765; METHIONINE--TRNA LIGASE; 1.
DR PANTHER; PTHR45765:SF1; METHIONINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR01041; TRNASYNTHMET.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363039};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363039};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363039};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363039};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363039}.
FT DOMAIN 8..371
FT /note="Methionyl/Leucyl tRNA synthetase"
FT /evidence="ECO:0000259|Pfam:PF09334"
SQ SEQUENCE 499 AA; 57621 MW; CEBDA6BA0D109DC3 CRC64;
MNTNKQTVVT AAFPFIPAEL CIAHIASTYL PADIFHRFEC LRGVNSILVS ATDVHGLLAK
KELSQAQMSS QELMQSWHRR YINEFSMLGI EFSHYGRTDA KALKELVYQS LHTLKEKGLI
TQREAFDYMC SDCGEMLPRR YRLAVKEMAA TGKEKIAYDD LDNDNLQCRF CGSRNVTKSS
KPHWFLDLQK GLPAIQENID KQKNNQVSTQ LKAAISDGLA DWNISRDNYM GTKLPFSDEQ
SLYLWYESLI GYLYLAECEN KYSNFEFKHF IGKNIIYYHG VVWPYLLHCG AERNDSEIQI
SARGFLDMEK TDSTLIDIQS ITSNYDRDYI RFYLAYRTPD SLSDYYFNQE DFSNTINQIF
CNQFGSFFRR CISILKKNQV EQVLPTELTN TPWETHWPLI ENALSQMLVR SALLHIQSYI
KACGTVIEDM SIYRTPTKAS LGLLSDLFIK ALFLLTPICP TRVQDFNIIN GLDLSRLRSH
EDCVGLPLRN SMARWEKID
//
