ID A0A066UH85_9VIBR Unreviewed; 200 AA.
AC A0A066UH85;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 24-JAN-2024, entry version 37.
DE RecName: Full=3-isopropylmalate dehydratase small subunit {ECO:0000256|HAMAP-Rule:MF_01031};
DE EC=4.2.1.33 {ECO:0000256|HAMAP-Rule:MF_01031};
DE AltName: Full=Alpha-IPM isomerase {ECO:0000256|HAMAP-Rule:MF_01031};
DE Short=IPMI {ECO:0000256|HAMAP-Rule:MF_01031};
DE AltName: Full=Isopropylmalate isomerase {ECO:0000256|HAMAP-Rule:MF_01031};
GN Name=leuD {ECO:0000256|HAMAP-Rule:MF_01031,
GN ECO:0000313|EMBL:KDN26771.1};
GN ORFNames=F2P58_15190 {ECO:0000313|EMBL:KAB0286086.1}, F2Z80_12535
GN {ECO:0000313|EMBL:KAB0304692.1}, VFDL14_08850
GN {ECO:0000313|EMBL:KDN26771.1};
OS Vibrio fortis.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=212667 {ECO:0000313|EMBL:KDN26771.1, ECO:0000313|Proteomes:UP000027219};
RN [1] {ECO:0000313|EMBL:KDN26771.1, ECO:0000313|Proteomes:UP000027219}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Dalian14 {ECO:0000313|EMBL:KDN26771.1,
RC ECO:0000313|Proteomes:UP000027219};
RA Wang Y., Song L., Liu G., Ding J.;
RT "Vibrio fortis Dalian14 Genome Sequencing.";
RL Submitted (FEB-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KAB0304692.1, ECO:0000313|Proteomes:UP000326687}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S7-72 {ECO:0000313|EMBL:KAB0304692.1,
RC ECO:0000313|Proteomes:UP000326687};
RA Das S.K.;
RT "Vibrio Fortis S7-72.";
RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:KAB0286086.1, ECO:0000313|Proteomes:UP000326789}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AN60 {ECO:0000313|EMBL:KAB0286086.1,
RC ECO:0000313|Proteomes:UP000326789};
RA Das S.K.;
RT "Whole genome sequence of Vibrio fortis.";
RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC isopropylmalate, via the formation of 2-isopropylmaleate.
CC {ECO:0000256|ARBA:ARBA00002695, ECO:0000256|HAMAP-Rule:MF_01031}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC EC=4.2.1.33; Evidence={ECO:0000256|ARBA:ARBA00000491,
CC ECO:0000256|HAMAP-Rule:MF_01031};
CC -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC from 3-methyl-2-oxobutanoate: step 2/4. {ECO:0000256|ARBA:ARBA00004729,
CC ECO:0000256|HAMAP-Rule:MF_01031}.
CC -!- SUBUNIT: Heterodimer of LeuC and LeuD. {ECO:0000256|ARBA:ARBA00011271,
CC ECO:0000256|HAMAP-Rule:MF_01031}.
CC -!- SIMILARITY: Belongs to the LeuD family. LeuD type 1 subfamily.
CC {ECO:0000256|ARBA:ARBA00009845, ECO:0000256|HAMAP-Rule:MF_01031}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KDN26771.1}.
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DR EMBL; VWSE01000008; KAB0286086.1; -; Genomic_DNA.
DR EMBL; VXDD01000001; KAB0304692.1; -; Genomic_DNA.
DR EMBL; JFFR01000028; KDN26771.1; -; Genomic_DNA.
DR RefSeq; WP_032553047.1; NZ_VXDD01000001.1.
DR AlphaFoldDB; A0A066UH85; -.
DR STRING; 212667.VFDL14_08850; -.
DR OrthoDB; 9777465at2; -.
DR UniPathway; UPA00048; UER00071.
DR Proteomes; UP000027219; Unassembled WGS sequence.
DR Proteomes; UP000326687; Unassembled WGS sequence.
DR Proteomes; UP000326789; Unassembled WGS sequence.
DR GO; GO:0009316; C:3-isopropylmalate dehydratase complex; IEA:InterPro.
DR GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd01577; IPMI_Swivel; 1.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR HAMAP; MF_01031; LeuD_type1; 1.
DR InterPro; IPR004431; 3-IsopropMal_deHydase_ssu.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR InterPro; IPR033940; IPMI_Swivel.
DR NCBIfam; TIGR00171; leuD; 1.
DR PANTHER; PTHR43345:SF5; 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT; 1.
DR PANTHER; PTHR43345; 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 2-RELATED-RELATED; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605, ECO:0000256|HAMAP-
KW Rule:MF_01031};
KW Branched-chain amino acid biosynthesis {ECO:0000256|ARBA:ARBA00023304,
KW ECO:0000256|HAMAP-Rule:MF_01031}; Isomerase {ECO:0000313|EMBL:KDN26771.1};
KW Leucine biosynthesis {ECO:0000256|ARBA:ARBA00022430, ECO:0000256|HAMAP-
KW Rule:MF_01031};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_01031}.
FT DOMAIN 1..125
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 200 AA; 22461 MW; FCA5BB71FE15C4FB CRC64;
MSGFKQHTGL VVPLDAANVD TDAIIPKQFL QKVSRLGFGK HLFHDWRFLD DAGQQPNPEF
VMNAPRYQGA SILLARENFG CGSSREHAPW ALADYGIKAM IAPSFADIFY GNSINNQMVP
VRLTEQEVDE IFQFVEANEG AEVEVDLEAN VVRANGKEYS FEIDEFRRHC LLNGLDNIGL
TLQHEEKISA YEANIPSFLK
//
