ID   A0A066WS66_9FLAO        Unreviewed;       639 AA.
AC   A0A066WS66;
DT   03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT   03-SEP-2014, sequence version 1.
DT   27-MAR-2024, entry version 25.
DE   SubName: Full=Endo-1,4-beta-xylanase {ECO:0000313|EMBL:KDN56872.1};
GN   ORFNames=FEM21_03750 {ECO:0000313|EMBL:KDN56872.1};
OS   Flavobacterium seoulense.
OC   Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC   Flavobacteriaceae; Flavobacterium.
OX   NCBI_TaxID=1492738 {ECO:0000313|EMBL:KDN56872.1, ECO:0000313|Proteomes:UP000027064};
RN   [1] {ECO:0000313|EMBL:KDN56872.1, ECO:0000313|Proteomes:UP000027064}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EM1321 {ECO:0000313|EMBL:KDN56872.1,
RC   ECO:0000313|Proteomes:UP000027064};
RA   Shin S.-K., Yi H.;
RT   "Genome Sequence of Flavobacterium sp. EM1321.";
RL   Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family.
CC       {ECO:0000256|ARBA:ARBA00009902}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC       {ECO:0000256|ARBA:ARBA00009865}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KDN56872.1}.
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DR   EMBL; JNCA01000001; KDN56872.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A066WS66; -.
DR   STRING; 1492738.FEM21_03750; -.
DR   PATRIC; fig|1492738.3.peg.369; -.
DR   eggNOG; COG3507; Bacteria.
DR   OrthoDB; 9763933at2; -.
DR   Proteomes; UP000027064; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0045493; P:xylan catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd08983; GH43_Bt3655-like; 1.
DR   CDD; cd18618; GH43_Xsa43E-like; 1.
DR   InterPro; IPR013148; Glyco_hydro_32_N.
DR   InterPro; IPR006710; Glyco_hydro_43.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   PANTHER; PTHR43772:SF2; BETA-1,4-XYLOSIDASE (EUROFUNG); 1.
DR   PANTHER; PTHR43772; ENDO-1,4-BETA-XYLANASE; 1.
DR   Pfam; PF00251; Glyco_hydro_32N; 1.
DR   Pfam; PF04616; Glyco_hydro_43; 1.
DR   SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 2.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000313|EMBL:KDN56872.1};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:KDN56872.1};
KW   Polysaccharide degradation {ECO:0000256|ARBA:ARBA00022651,
KW   ECO:0000313|EMBL:KDN56872.1}; Signal {ECO:0000256|SAM:SignalP};
KW   Xylan degradation {ECO:0000256|ARBA:ARBA00022651,
KW   ECO:0000313|EMBL:KDN56872.1}.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           22..639
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5001633193"
FT   DOMAIN          21..124
FT                   /note="Glycosyl hydrolase family 32 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00251"
FT   ACT_SITE        363
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   ACT_SITE        532
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   SITE            484
FT                   /note="Important for catalytic activity, responsible for
FT                   pKa modulation of the active site Glu and correct
FT                   orientation of both the proton donor and substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ   SEQUENCE   639 AA;  73123 MW;  0C302772B823BDF2 CRC64;
     MNKKIYHIVL LFVLGLSNSI AQNQKKEAYL FAYFEGKGAA EKQEQLRFAV SDDAVNWKAL
     NNNDPVIPSK DISNTGGIRD PHILRGADGK SFYMVATDMN TKKNGWDYNP GITLLKSNNL
     IDWKSGVIDL EKLYPKKFKD IKWVWAPQTF YDAKVKKYLV YFTVRFKYNE KLDFYCAYAN
     KDFTAFESEP KLMFSPKDGA IDGDIIYKDG VYHFFYKGNT KDENGKEIKN GIKQATSKKL
     QGPWKESFEY LDAYANTKTG VEGSSVFKLN NSDEYILMYD LYSSGRYEFQ RSKDLYHFTE
     KTESFTKNFF PRHGSVIGIT KEEAQRLNEK WGGVPQSLLT TKEGCYRFKS EGNPIIKHKF
     TADPAAFVEK DTLWLFTGHD FEGGQKGYKL KDWLVFSTTD LKNWTEYPVP LKIEDFKWDK
     TNDAYAGQAI KRNGKYYWYI STNGSGIGVA VADKPQGPYK DALGKPLLTN ADCFASTHSW
     TCIDPAVFID DNGQAWLFWG NGVCYYAKLK ENMIEIDGAI KKIDFEGFQF TEALWIHKKD
     GKYYLSYATG FPEKIAYAMA DNIEGPYEYK GILNEITGNS NTNHQAILEF KGQPYFIYHN
     GAMQTNGSSY SRSVCIDKLE YNQDGTIKRV VMTTEGVVK
//