ID A0A067BX23_SAPPC Unreviewed; 498 AA.
AC A0A067BX23;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=phosphoethanolamine N-methyltransferase {ECO:0000256|ARBA:ARBA00035674};
DE EC=2.1.1.103 {ECO:0000256|ARBA:ARBA00035674};
GN ORFNames=SPRG_15850 {ECO:0000313|EMBL:KDO18851.1};
OS Saprolegnia parasitica (strain CBS 223.65).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Saprolegniales; Saprolegniaceae;
OC Saprolegnia.
OX NCBI_TaxID=695850 {ECO:0000313|EMBL:KDO18851.1, ECO:0000313|Proteomes:UP000030745};
RN [1] {ECO:0000313|EMBL:KDO18851.1, ECO:0000313|Proteomes:UP000030745}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 223.65 {ECO:0000313|EMBL:KDO18851.1,
RC ECO:0000313|Proteomes:UP000030745};
RX PubMed=23785293;
RA Jiang R.H., de Bruijn I., Haas B.J., Belmonte R., Lobach L., Christie J.,
RA van den Ackerveken G., Bottin A., Bulone V., Diaz-Moreno S.M., Dumas B.,
RA Fan L., Gaulin E., Govers F., Grenville-Briggs L.J., Horner N.R.,
RA Levin J.Z., Mammella M., Meijer H.J., Morris P., Nusbaum C., Oome S.,
RA Phillips A.J., van Rooyen D., Rzeszutek E., Saraiva M., Secombes C.J.,
RA Seidl M.F., Snel B., Stassen J.H., Sykes S., Tripathy S., van den Berg H.,
RA Vega-Arreguin J.C., Wawra S., Young S.K., Zeng Q., Dieguez-Uribeondo J.,
RA Russ C., Tyler B.M., van West P.;
RT "Distinctive expansion of potential virulence genes in the genome of the
RT oomycete fish pathogen Saprolegnia parasitica.";
RL PLoS Genet. 9:E1003272-E1003272(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N,N-dimethylethanolamine phosphate + S-adenosyl-L-methionine =
CC H(+) + phosphocholine + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:25325, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856,
CC ChEBI:CHEBI:58641, ChEBI:CHEBI:59789, ChEBI:CHEBI:295975;
CC EC=2.1.1.103; Evidence={ECO:0000256|ARBA:ARBA00035588};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:25326;
CC Evidence={ECO:0000256|ARBA:ARBA00035588};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N-methylethanolamine phosphate + S-adenosyl-L-methionine =
CC H(+) + N,N-dimethylethanolamine phosphate + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:25321, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57781, ChEBI:CHEBI:57856, ChEBI:CHEBI:58641,
CC ChEBI:CHEBI:59789; EC=2.1.1.103;
CC Evidence={ECO:0000256|ARBA:ARBA00035580};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:25322;
CC Evidence={ECO:0000256|ARBA:ARBA00035580};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=phosphoethanolamine + S-adenosyl-L-methionine = H(+) + N-
CC methylethanolamine phosphate + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:20365, ChEBI:CHEBI:15378, ChEBI:CHEBI:57781,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:58190, ChEBI:CHEBI:59789;
CC EC=2.1.1.103; Evidence={ECO:0000256|ARBA:ARBA00035571};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20366;
CC Evidence={ECO:0000256|ARBA:ARBA00035571};
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- PATHWAY: Phospholipid metabolism; phosphatidylcholine biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004969}.
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DR EMBL; KK583388; KDO18851.1; -; Genomic_DNA.
DR RefSeq; XP_012210434.1; XM_012355044.1.
DR AlphaFoldDB; A0A067BX23; -.
DR STRING; 695850.A0A067BX23; -.
DR EnsemblProtists; KDO18851; KDO18851; SPRG_15850.
DR GeneID; 24137534; -.
DR KEGG; spar:SPRG_15850; -.
DR VEuPathDB; FungiDB:SPRG_15850; -.
DR OMA; KSYWTEH; -.
DR OrthoDB; 2249143at2759; -.
DR Proteomes; UP000030745; Unassembled WGS sequence.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0044249; P:cellular biosynthetic process; IEA:UniProt.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:UniProt.
DR GO; GO:1901576; P:organic substance biosynthetic process; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 2.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR InterPro; IPR025714; Methyltranfer_dom.
DR InterPro; IPR041698; Methyltransf_25.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR44307; PHOSPHOETHANOLAMINE METHYLTRANSFERASE; 1.
DR PANTHER; PTHR44307:SF2; PHOSPHOETHANOLAMINE METHYLTRANSFERASE ISOFORM X1; 1.
DR Pfam; PF13649; Methyltransf_25; 1.
DR Pfam; PF13847; Methyltransf_31; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 2.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000030745};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 60..153
FT /note="Methyltransferase"
FT /evidence="ECO:0000259|Pfam:PF13649"
FT DOMAIN 287..393
FT /note="Methyltransferase"
FT /evidence="ECO:0000259|Pfam:PF13847"
SQ SEQUENCE 498 AA; 55407 MW; 044BF37A8F9840E8 CRC64;
MAGLDEATVA TRNDMKSYWS EHSQETTIET MMLDSNAKVL HELEIPEILA KLPDVKDKDV
LEMAAGIGRY TGRIADAAAS VTAVEFIEDF HKANEKSNGH RGNVSFLCAD VCDLERQPSS
FDVIFSNWLL MYLDDDEVAR LAQKTLKWLR PGGEVFFRES CFRQSGDVKR TSNPTKYRHP
SFYVGAFASA VIDEGNGRVS QFELVSSSSV GVYRRVKKNN GQICFRFKKV IKEGSVGAAE
TFQRFLDAQQ YSRGSIARYE KIFGAGYIST GGQDTTTEFV AKLGLVRGER VLDVGCGIGG
GDFYMAKTFG VSVVGIDLST NMVHRALETS LTDPSVDCEF EICDATTKEY APASFDVIYS
RDTILHIADK KSLFAKFYTW LKPGGRLLIS DYCCGEAETQ TTRFKAYVAS RGYHLLTPAA
YGHVIASVGF SDVEAEDRTE HFVQVLQTEL ARTRLNKDAF IAETSAHDYE DIVDGWEAKL
VRCAEGDQKW GLFFARKA
//
