ID A0A067CFU4_SAPPC Unreviewed; 2176 AA.
AC A0A067CFU4;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 24-JAN-2024, entry version 40.
DE RecName: Full=1,3-beta-glucan synthase {ECO:0000256|ARBA:ARBA00012589};
DE EC=2.4.1.34 {ECO:0000256|ARBA:ARBA00012589};
GN ORFNames=SPRG_20212 {ECO:0000313|EMBL:KDO28050.1};
OS Saprolegnia parasitica (strain CBS 223.65).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Saprolegniales; Saprolegniaceae;
OC Saprolegnia.
OX NCBI_TaxID=695850 {ECO:0000313|EMBL:KDO28050.1, ECO:0000313|Proteomes:UP000030745};
RN [1] {ECO:0000313|EMBL:KDO28050.1, ECO:0000313|Proteomes:UP000030745}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 223.65 {ECO:0000313|EMBL:KDO28050.1,
RC ECO:0000313|Proteomes:UP000030745};
RX PubMed=23785293;
RA Jiang R.H., de Bruijn I., Haas B.J., Belmonte R., Lobach L., Christie J.,
RA van den Ackerveken G., Bottin A., Bulone V., Diaz-Moreno S.M., Dumas B.,
RA Fan L., Gaulin E., Govers F., Grenville-Briggs L.J., Horner N.R.,
RA Levin J.Z., Mammella M., Meijer H.J., Morris P., Nusbaum C., Oome S.,
RA Phillips A.J., van Rooyen D., Rzeszutek E., Saraiva M., Secombes C.J.,
RA Seidl M.F., Snel B., Stassen J.H., Sykes S., Tripathy S., van den Berg H.,
RA Vega-Arreguin J.C., Wawra S., Young S.K., Zeng Q., Dieguez-Uribeondo J.,
RA Russ C., Tyler B.M., van West P.;
RT "Distinctive expansion of potential virulence genes in the genome of the
RT oomycete fish pathogen Saprolegnia parasitica.";
RL PLoS Genet. 9:E1003272-E1003272(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1->3)-
CC beta-D-glucosyl](n+1) + H(+) + UDP; Xref=Rhea:RHEA:21476, Rhea:RHEA-
CC COMP:11146, Rhea:RHEA-COMP:14303, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:37671, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885; EC=2.4.1.34;
CC Evidence={ECO:0000256|ARBA:ARBA00000192};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 48 family.
CC {ECO:0000256|ARBA:ARBA00009040}.
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DR EMBL; KK583213; KDO28050.1; -; Genomic_DNA.
DR RefSeq; XP_012201201.1; XM_012345811.1.
DR STRING; 695850.A0A067CFU4; -.
DR EnsemblProtists; KDO28050; KDO28050; SPRG_20212.
DR GeneID; 24141409; -.
DR KEGG; spar:SPRG_20212; -.
DR VEuPathDB; FungiDB:SPRG_20212; -.
DR OMA; ITNFQWH; -.
DR OrthoDB; 48003at2759; -.
DR Proteomes; UP000030745; Unassembled WGS sequence.
DR GO; GO:0000148; C:1,3-beta-D-glucan synthase complex; IEA:InterPro.
DR GO; GO:0003843; F:1,3-beta-D-glucan synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006075; P:(1->3)-beta-D-glucan biosynthetic process; IEA:InterPro.
DR Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 1.
DR InterPro; IPR026899; FKS1-like_dom1.
DR InterPro; IPR003440; Glyco_trans_48.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR12741:SF29; 1,3-BETA-GLUCAN SYNTHASE COMPONENT FKS1-RELATED; 1.
DR PANTHER; PTHR12741; LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1; 1.
DR Pfam; PF14288; FKS1_dom1; 1.
DR Pfam; PF02364; Glucan_synthase; 2.
DR Pfam; PF00083; Sugar_tr; 1.
DR SMART; SM01205; FKS1_dom1; 1.
DR SUPFAM; SSF103473; MFS general substrate transporter; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000030745};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 233..256
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 284..306
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 318..341
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 361..387
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 408..431
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 470..494
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1218..1236
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1269..1287
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1294..1312
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1364..1384
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1418..1439
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1522..1543
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1555..1575
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1587..1607
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1627..1649
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1669..1698
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1734..1755
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1762..1779
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1785..1809
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1821..1839
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1859..1876
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1916..1940
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1960..1977
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1989..2009
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2021..2046
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2058..2076
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2082..2103
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 94..210
FT /note="1,3-beta-glucan synthase component FKS1-like"
FT /evidence="ECO:0000259|SMART:SM01205"
FT REGION 2128..2154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2176 AA; 243273 MW; 641BC91C1F1A5460 CRC64;
MLHQDFRKSV KRLDSGHHGG VFEHLQRLLG FQFASMRNQK EHVAVLLTNK QTRLHNKGSA
DPARDALKQL HSAVFTNYTK WCKFLGTKPR FVGASQEAQI ALYFLIWGEA GNLRFMPECL
CFLYHTMASR WEKLEALGHK PEMAPADTAF LNNVVKPLYK VVAKMNPALS SKSKKDDGKP
KDHKNVTNYD DVNEFFWTPE CLKFDETNVA RAFERREVKT FKERRSLLSP FLAFYRIFFF
LLVMLHVLIA IAYVGVKTNN GNGSGFHIYD DFFSSVYSNL RLHAFYSIAA SVAGLLALKV
VLQVWIDGAL IYSHKMYALA LYVRLLWHSL FLALFCCMIC SPNETDMVLG KDYLSAAPIY
IGVYALPVVL GALVSMCCGS MHLPFLRALQ GTRSQYIGRE MGQPFGDFVK YALFWILLFG
LKFVFNLQLM IMPLMNPSIE LYALHVESQS SLLNSDHNLL FIAALWAPTF LVYIYDTQIW
LAIFQAIIGM FMGFRMKIGH SSRVTQFMAK LQGAPTLFDQ KVVSRVARGM LAATAEGSSS
PEVQARLRFS VVWNECVSSF RLSDLIDDRE SAILQYQISA SGQVDDPVFL LAGKTSRACD
ILGGIKSPSR SKIQKDLSKD GLVNVTINAL DLGVRILTKL LGPEDAAIID GLQHAIANAD
ETLRSANLTH VPQLRENAVD LLACLLDMPD AEDELPTNAR VGSQVLQDRV SLVVERVQHV
FGTLQQVLPS DWMRQRLAGC KFTQLTSDMT HQMTLLSSLF VDEEVASDDD NSDCVHAADF
LLTLDIADAL PRCAEAQRRM SFFLNSLAMY IPQVDAIQSM HSFSVVTPYY NEPVLYSLEE
LNDRVVVNPL FRAAEAKAKK DKVGREDLSI LKYLITLHPE EWGNFLERLG VSSREEALVS
SPTQVRLWAS MRGQTLARTV HGMMLYEDAI KILRWLEIGS DQSMSHDEKI EQMDHIVGMK
FSYITSCQIY AAQSASNDPR AADIDLLMRK YPNWRVSFME QVGDSYECVL VKADGDDIVE
VYRYELPGHP IVGEGKPENQ NIALPFTRGE YIQTIDMNQE HYFEEALKMP NFLATGKNVQ
IIGMKEYIFT AKASSLARFM TLQELVFVSL TQRVMANPLQ TRMHYGHPDV FDKLYIMTNG
GVSKASKGIN LSEDVFAGYS CALRGGKVTH VEFMQCGKGR DVTLSQINAF EAKLANGGAP
HGRGHGFFRL NAMYYSHMGF YIANFLTVVC VFVYAYCKLY VALHLDVEQA VIPVTSDLDH
LAQVMNTQFI FQFGMLMTIP LVATLVVEVG LRQAVIQFCE LIITLGPVFY IFETGTKAHF
FDVAIQRGGS KYRGTGRGFA ITRETFVNFF KEYAASHYRK ALELMALMVL FGLYGTFNIG
LGALDAYCPK DGNNTECQGN PPSTINNLDS YGKKGQSYGV ASFAVWLLVV CWFFAPFIFN
TDGFDFTKTR MDVLNWFDWL GTTSAADDSK SDSWAKWWQS EADLYKPISW TSRLSYALRE
FRHILVVYYI FTYSFELSKI GLLLAAIGGV ALALWLGGTF SRILFGNKLR GPRGLLYLAI
VLGITIGGVV AVGSLSSWSG MKTLSFFVAM YAGLYSLLQY CLVLHGLCGL PIARWGLVQE
LGYLFDSLLG LLLLLPLVIL SAIPFMHTIQ TRMMYNEGFS KTLSTGGEYA ASISMFTGIF
GGFTHGWLLC ALFSLGFLND TKWIALNESF AKFIGDEGFK WASEAAKRDF QTSLSLFAGI
GCLAGVVFGV GLCFAVGRRT TFVLSSALTL VGVGLMWAVT TTSSMVVVAV MVLGAAIAMS
SIAICLYNYE ICTRDWKCKA VVVFVFGSAL GYFVESLLLY QWNYHLLEVG WKEDKINMWR
LQFIYGAIPS SPVWLLRRGN KKGAEATLVR LRQKHDILDE LQAINDAMYP KDPPNLAFRA
LLVILLQVMY ALVSSGTLFL RVLVQGTTNA LGAPASPWQW YYGLMSMLGC LMGFFTIDAI
RRKTLLKDVL PLVSLLSVAA LVLQNSLFSE ADALKAKDPP VLLSVVLFLF YFTVAYSVST
ATWLVAIEVF PGGCQLRFVL LSFLVYYGVH TALAFVRPNF MTAQIAFAAL AIGTTIVLFV
FAASNQRGAI QLKHEKTKEQ AAIKAAEEDE LQRNTRASRS HSFLRSRADR KSSFKDKDAY
HAFESPAATS VKPRVV
//
