ID   A0A067F6Q7_CITSI        Unreviewed;       574 AA.
AC   A0A067F6Q7;
DT   03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT   03-SEP-2014, sequence version 1.
DT   27-MAR-2024, entry version 26.
DE   RecName: Full=cysteine--tRNA ligase {ECO:0000256|ARBA:ARBA00012832};
DE            EC=6.1.1.16 {ECO:0000256|ARBA:ARBA00012832};
DE   AltName: Full=Cysteinyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00031499};
GN   ORFNames=CISIN_1g008191mg {ECO:0000313|EMBL:KDO61805.1};
OS   Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX   NCBI_TaxID=2711 {ECO:0000313|EMBL:KDO61805.1, ECO:0000313|Proteomes:UP000027120};
RN   [1] {ECO:0000313|EMBL:KDO61805.1, ECO:0000313|Proteomes:UP000027120}
RP   NUCLEOTIDE SEQUENCE.
RG   International Citrus Genome Consortium;
RA   Gmitter F., Chen C., Farmerie W., Harkins T., Desany B., Mohiuddin M.,
RA   Kodira C., Borodovsky M., Lomsadze A., Burns P., Jenkins J., Prochnik S.,
RA   Shu S., Chapman J., Pitluck S., Schmutz J., Rokhsar D.;
RL   Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|ARBA:ARBA00001947};
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000256|ARBA:ARBA00005594}.
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DR   EMBL; KK784923; KDO61805.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A067F6Q7; -.
DR   STRING; 2711.A0A067F6Q7; -.
DR   PaxDb; 2711-XP_006483408-1; -.
DR   eggNOG; KOG2007; Eukaryota.
DR   Proteomes; UP000027120; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR   GO; GO:0004817; F:cysteine-tRNA ligase activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006423; P:cysteinyl-tRNA aminoacylation; IBA:GO_Central.
DR   CDD; cd00672; CysRS_core; 1.
DR   Gene3D; 1.20.120.1910; Cysteine-tRNA ligase, C-terminal anti-codon recognition domain; 1.
DR   Gene3D; 3.40.50.620; HUPs; 1.
DR   HAMAP; MF_00041; Cys_tRNA_synth; 1.
DR   InterPro; IPR015803; Cys-tRNA-ligase.
DR   InterPro; IPR024909; Cys-tRNA/MSH_ligase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR032678; tRNA-synt_1_cat_dom.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   NCBIfam; TIGR00435; cysS; 1.
DR   PANTHER; PTHR10890:SF3; CYSTEINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR   PANTHER; PTHR10890; CYSTEINYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF01406; tRNA-synt_1e; 1.
DR   PRINTS; PR00983; TRNASYNTHCYS.
DR   SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR   SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW   Reference proteome {ECO:0000313|Proteomes:UP000027120};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   DOMAIN          90..385
FT                   /note="tRNA synthetases class I catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF01406"
SQ   SEQUENCE   574 AA;  64849 MW;  A725F0BAF76981B0 CRC64;
     MGSLILTLKC YKPLASIKFS PFINSFHTLQ LRPPKPNNRR VFRCFSTLNS SPNVIDDKSN
     GKTDGDDKPA SASTQLWLYN TMSRKRELVK PKVEGKVGMY VCGVTPYDLS HIGHARVYVT
     FDVLYRYLRH LGYEVCYVRN FTDVDDKIIA RANELGEDPI SLSRRYCEEF HQDMVDLHCL
     PPSVEPRVSD HMPHIIDMIT QILDNGFAYR VDGDVYFSVD KFPEYGQLSG RKLEDNRAGE
     RVAVDSRKKN PADFALWKSA KEGEPFWESP WGPGRPGWHI ECSAMSAAYL GHSFDIHGGG
     MDLVFPHHEN EIAQSCAACN NSHISYWIHN GFVTIDSEKM SKSLGNFFTI RQVMELYHPL
     ALRLFLIGTH YRSPINYSVV QLESASERIF YIYQTLHDCE KALSLLDAAA LKDSIPPDTV
     TSINKFHNDF LASMSDDLHT TVVLAALSDP LKTINDLLHT RKGKKQAMRL ESLAALEKTI
     RNVLTVLGLM STGYSEVLLQ LREKALKRAD LTEDQVLQRI EDRTVARKNK EYEKSDAIRK
     DLAAVGIALM DSPVGTTWRP ALPLALQEQA VATS
//