ID A0A067FA78_CITSI Unreviewed; 361 AA.
AC A0A067FA78;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=feruloyl-CoA 6-hydroxylase {ECO:0000256|ARBA:ARBA00012885};
DE EC=1.14.11.61 {ECO:0000256|ARBA:ARBA00012885};
GN ORFNames=CISIN_1g018077mg {ECO:0000313|EMBL:KDO63055.1};
OS Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX NCBI_TaxID=2711 {ECO:0000313|EMBL:KDO63055.1, ECO:0000313|Proteomes:UP000027120};
RN [1] {ECO:0000313|EMBL:KDO63055.1, ECO:0000313|Proteomes:UP000027120}
RP NUCLEOTIDE SEQUENCE.
RG International Citrus Genome Consortium;
RA Gmitter F., Chen C., Farmerie W., Harkins T., Desany B., Mohiuddin M.,
RA Kodira C., Borodovsky M., Lomsadze A., Burns P., Jenkins J., Prochnik S.,
RA Shu S., Chapman J., Pitluck S., Schmutz J., Rokhsar D.;
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-feruloyl-CoA + 2-oxoglutarate + O2 = (E)-6-
CC hydroxyferuloyl-CoA + CO2 + succinate; Xref=Rhea:RHEA:57856,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:16810,
CC ChEBI:CHEBI:30031, ChEBI:CHEBI:87305, ChEBI:CHEBI:142390;
CC EC=1.14.11.61; Evidence={ECO:0000256|ARBA:ARBA00000471};
CC -!- COFACTOR:
CC Name=L-ascorbate; Xref=ChEBI:CHEBI:38290;
CC Evidence={ECO:0000256|ARBA:ARBA00001961};
CC -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC family. {ECO:0000256|RuleBase:RU003682}.
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DR EMBL; KK784915; KDO63055.1; -; Genomic_DNA.
DR RefSeq; XP_006488907.1; XM_006488844.2.
DR AlphaFoldDB; A0A067FA78; -.
DR SMR; A0A067FA78; -.
DR STRING; 2711.A0A067FA78; -.
DR PaxDb; 2711-XP_006488907-1; -.
DR GeneID; 102613413; -.
DR KEGG; cit:102613413; -.
DR eggNOG; KOG0143; Eukaryota.
DR OrthoDB; 1211890at2759; -.
DR Proteomes; UP000027120; Unassembled WGS sequence.
DR GO; GO:0051213; F:dioxygenase activity; IEA:UniProt.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0044550; P:secondary metabolite biosynthetic process; IEA:UniProt.
DR InterPro; IPR026992; DIOX_N.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR027443; IPNS-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR PANTHER; PTHR10209:SF243; FERULOYL COA ORTHO-HYDROXYLASE 1-RELATED; 1.
DR PANTHER; PTHR10209; OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN; 1.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR Pfam; PF14226; DIOX_N; 1.
DR SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU003682};
KW Metal-binding {ECO:0000256|RuleBase:RU003682};
KW Oxidoreductase {ECO:0000256|RuleBase:RU003682};
KW Reference proteome {ECO:0000313|Proteomes:UP000027120}.
FT DOMAIN 208..309
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000259|PROSITE:PS51471"
SQ SEQUENCE 361 AA; 40272 MW; 7D9782F1AFB0D95A CRC64;
MAPTTVLLSD FSDVTDFVVN QGNGVKGLSE MGLKALPKQY IQPLEERFSE KKIVPHESIP
IIDMSKWDDP EVAKSICDAA EMWGFFQVVN HRVPLQVLDR VKAATHRFFG LSAAEKNKYS
KELSPSNNVR FGTSFNTQAE KALEWKDYLS LFYVSDDEAS ALWPPACKDE VLEYTKSSEV
LIKQLLKVLM KGLKVEGTDE AKEALLMGSV RTNLNYYPIC PNPELTVGVG RHSDVSTLTI
LLQDDIGGLY VRGNDGESWI HVPPISGSLV INIGDALQIM SNGKYRSVEH CVIANGSSNR
ISVPIFINPR PQDTIGPFPE VLASGEKPVY KQVLYKDYVK HFFRKAHDGK KTVDFAKLSI
F
//
