ID A0A067H2F3_CITSI Unreviewed; 467 AA.
AC A0A067H2F3;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 24-JAN-2024, entry version 25.
DE RecName: Full=digalactosyldiacylglycerol synthase {ECO:0000256|ARBA:ARBA00024055};
DE EC=2.4.1.241 {ECO:0000256|ARBA:ARBA00024055};
GN ORFNames=CISIN_1g012256mg {ECO:0000313|EMBL:KDO86049.1};
OS Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX NCBI_TaxID=2711 {ECO:0000313|EMBL:KDO86049.1, ECO:0000313|Proteomes:UP000027120};
RN [1] {ECO:0000313|EMBL:KDO86049.1, ECO:0000313|Proteomes:UP000027120}
RP NUCLEOTIDE SEQUENCE.
RG International Citrus Genome Consortium;
RA Gmitter F., Chen C., Farmerie W., Harkins T., Desany B., Mohiuddin M.,
RA Kodira C., Borodovsky M., Lomsadze A., Burns P., Jenkins J., Prochnik S.,
RA Shu S., Chapman J., Pitluck S., Schmutz J., Rokhsar D.;
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + UDP-alpha-
CC D-galactose = 1,2-diacyl-3-O-[alpha-D-galactosyl-(1->6)-beta-D-
CC galactosyl]-sn-glycerol + H(+) + UDP; Xref=Rhea:RHEA:10520,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17615, ChEBI:CHEBI:28396,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:66914; EC=2.4.1.241;
CC Evidence={ECO:0000256|ARBA:ARBA00023914};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004370}.
CC Plastid, chloroplast outer membrane {ECO:0000256|ARBA:ARBA00024013}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC Glycosyltransferase 4 subfamily. {ECO:0000256|ARBA:ARBA00009481}.
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DR EMBL; KK784873; KDO86049.1; -; Genomic_DNA.
DR EMBL; KK784873; KDO86050.1; -; Genomic_DNA.
DR STRING; 2711.A0A067H2F3; -.
DR PaxDb; 2711-XP_006491041-1; -.
DR eggNOG; ENOG502QQEJ; Eukaryota.
DR Proteomes; UP000027120; Unassembled WGS sequence.
DR GO; GO:0009707; C:chloroplast outer membrane; IBA:GO_Central.
DR GO; GO:0046481; F:digalactosyldiacylglycerol synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0035250; F:UDP-galactosyltransferase activity; IBA:GO_Central.
DR GO; GO:0019375; P:galactolipid biosynthetic process; IBA:GO_Central.
DR CDD; cd01635; Glycosyltransferase_GTB-type; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 1.
DR InterPro; IPR044525; DGDG1/2.
DR InterPro; IPR001296; Glyco_trans_1.
DR PANTHER; PTHR46132; DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTIC; 1.
DR PANTHER; PTHR46132:SF1; DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTIC; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Membrane {ECO:0000256|ARBA:ARBA00022805};
KW Plastid {ECO:0000256|ARBA:ARBA00022805};
KW Plastid outer membrane {ECO:0000256|ARBA:ARBA00022805};
KW Reference proteome {ECO:0000313|Proteomes:UP000027120};
KW Transferase {ECO:0000256|ARBA:ARBA00022676}.
FT DOMAIN 220..335
FT /note="Glycosyl transferase family 1"
FT /evidence="ECO:0000259|Pfam:PF00534"
SQ SEQUENCE 467 AA; 53106 MW; CB5B9E989478057E CRC64;
MDRKQQHIAI FTTASLPWLT GTAVNPLFRA AYLAKDGETR VTLVIPWLSL IDQKLVYPEN
ITFTSPKQHE TYVRRWLEER TGFTSTFDIR FYPGKFAIDK RSILGVGDIS EVIPDEVADI
AVLEEPEHLT WFHHGKRWKT KFRYVVGIVH TNYLEYVKRE KNGRLQAFLL KYANSWLVDI
YCHKVIRLSA ATQEYANSII CNVHGVNPKF LEIGKKKKEQ QQNGTHAFAK GAYYIGKMVW
SKGYKELLEL LDDHQKELAG LEVDLYGNGE DFNQIQEAAE KLKIVVRVYP GRDHADLIFH
DYKVFLNPST TDVVCTTTAE ALAMGKIVVC ANHPSNDFFK QFPNCRTYDD RNGFVEATLK
ALAEEPALPT EAQRHQLSWE SATERFLQVA ELDQAVVKKP SKSPSKHFAS TSLNLKKNME
EASAYVHFLA SGFETSRRAF GAIPGSLHPD EELCKELGLV TPMSKQG
//
